Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms

Patange, Apurva; Simpson, Jeremy C.; Curtin, James F.; Burgess, Catherine M.; Cullen, Patrick J.; Tiwari, Brijesh K.

doi:10.1038/s41598-021-81977-z

Cited by 23 publications

(16 citation statements)

References 41 publications

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“…EM is a drug with significant anti-inflammatory and antibacterial activity (37,38), thus it has potential to replace antibiotics as an effective drug for treating acute osteomyelitis. Previous studies have reported the antibacterial effect of ultrasound in food packaging (39), and LFLIU has also been reported to stimulate ROS production, which provides a specific repressive effect on the growth of suspended bacteria and bacterial biofilms (12,13,40). In this study, it found that M-LFLIU combined with EM had significant synergistic antibacterial effects in vitro and in vivo, providing a promising possibility for alternative clinical treatments of acute osteomyelitis.…”

Section: Discussionmentioning

confidence: 50%

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

et al. 2022

Preprint

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Treatment of osteomyelitis is still challenging as conventional antibiotic therapy is limited by the emergence of resistant strains and the formation of biofilms. Sonoantimicrobial chemotherapy (SACT) is a novel therapy of low-frequency and low-intensity ultrasound (LFLIU) combined with sonosensitizer. Therefore, in our study, a sonosensitizer named emodin (EM) was proposed to be combined with LFLIU to relieve acute osteomyelitis caused by Methicillin-resistant Staphylococcus aureus (MRSA) through synergistic antibacterial and anti-biofilm effects. The efficiency of different intensities of ultrasound, single (S-LFLIU, 15 min) and multiple ultrasound (M-LFLIU, 5 min every 4 h, three times) against bacteria and biofilm was compared, contributing to develop the best treatment regimen. Our results demonstrated that EM plus S-LFLIU or M-LFLIU (EM+S-LFLIU or EM+M-LFLIU) have significant synergetic bactericidal and anti-biofilm effects and EM+M-LFLIU exhibits superior performance in anti-biofilm. Furthermore, it was suggested that EM+M-LFLIU could produce a large amount of reactive oxygen species (ROS), destroy the integrity of bacterial membrane and wall, down-regulate the expression of oxidative stress, membrane wall synthesis, bacterial virulence and other related genes (agrB, PBP3, sgtB, GMK, zwf, msrA). In vivo study, micro-CT, H&E staining, ELISA assay and bacterial quantification of bone tissue indicated that EM+M-LFLIU could also relieve osteomyelitis of MRSA infection. Our work proffers an original treating bacterial osteomyelitis approach that weakens drug-resistant bacterial and suppresses biofilm formation through SACT, which may provide new prospects for clinical treatment.

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Section: Discussionmentioning

confidence: 50%

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

et al. 2022

Preprint

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“…However, as PAW is yet a novel technology, a detailed investigation of its mechanism of action and potential interactions with EPS matrix and other biofilm compounds is yet to be performed. It is well known that biofilm maturity significantly affects plasma penetration because thicker biofilms with more biomass provide a better protection against reactive species (Chen et al, 2017; Hathaway et al, 2019; Patange et al, 2021). The same effect was observed in the current study, when the 72 h mature biofilms were found to be most resistant against PAW treatment.…”

Section: Discussionmentioning

confidence: 99%

“…PAW is considered a promising technology, showing apparent suitability to substitute more traditional treatments used in a wide range of sectors, for example chlorine‐based (Patange et al, 2021; Xiang et al, 2018). However, as PAW is yet a novel technology, a detailed investigation of its mechanism of action and potential interactions with EPS matrix and other biofilm compounds is yet to be performed.…”

Section: Discussionmentioning

confidence: 99%

“…This study showed that PAW treatment significantly reduces the level of bacterial cells and that this reduction depended on the maturity of the biofilms. Patange et al (2021) demonstrated inactivation of early and mature Escherichia coli and Listeria innocua biofilm cells by atmospheric air plasma (AAP). They showed that a 5 min AAP treatment reduced the cell count by approximately 3.5–4.5 Log CFU ml −1 .…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

Asimakopoulou

Εkonomou

Papakonstantinou

et al. 2022

Journal of Applied Microbiology

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Aims:The cost of Microbiologically Influenced Corrosion (MIC) significantly affects a wide range of sectors. This study aims to assess the efficiency of a novel technology based on the use of plasma-activated water (PAW) in inhibiting corrosion caused by bacteria. Methods and Results:This study evaluated the effectiveness of PAW, produced by a plasma bubble reactor, in reducing corrosion causing Pseudomonas aeruginosa planktonic cells in tap water and biofilms were grown onto stainless steel (SS) coupons. Planktonic cells and biofilms were treated with PAW at different discharge frequencies (500-1500 Hz) and exposure times (0-20 min). P. aeruginosa cells in tap water were significantly reduced after treatment, with higher exposure times and discharge frequencies achieving higher reductions. Also, PAW treatment led to a gradual reduction for young and mature biofilms, achieving >4-Log reductions after 20 min. Results were also used to develop two predictive inactivation models. Conclusions:This work presents evidence that PAW can be used to inactivate both planktonic cells and biofilms of P. aeruginosa. Experimental and theoretical results also demonstrate that reduction is dependent on discharge frequency and exposure time.Significance and Impact of the Study: This work demonstrates the potential of using PAW as means to control MIC.

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“…Atmospheric air plasma damaged both the bacterial biofilm cells and its structural integrity. Scanning electron microscopy envinced the disruption of biofilms and pore formation in bacterial cells after exposure to plasma treatment [104]. The elevated reactive oxygen and nitrogen species in bacterial cells treated with atmospheric air plasma demonstrated their primary role in the observed bacterial inactivation process.…”

Section: Effects Of Cold Plasma Technology On Biofilm Formation On Fo...mentioning

confidence: 97%

A Cold Plasma Technology for Ensuring the Microbiological Safety and Quality of Foods

et al. 2022

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Changing consumers’ taste for chemical and thermally processed food and preference for perceived healthier minimally processed alternatives is a challenge to food industry. At present, several technologies have found usefulness as choice methods for ensuring that processed food remains unaltered while guaranteeing maximum safety and protection of consumers. However, the effectiveness of most green technology is limited due to the formation of resistant spores by certain foodborne microorganisms and the production of toxins. Cold plasma, a recent technology, has shown commendable superiority at both spore inactivation and enzymes and toxin deactivation. However, the exact mechanism behind the efficiency of cold plasma has remained unclear. In order to further optimize and apply cold plasma treatment in food processing, it is crucial to understand these mechanisms and possible factors that might limit or enhance their effectiveness and outcomes. As a novel non-thermal technology, cold plasma has emerged as a means to ensure the microbiological safety of food. Furthermore, this review presents the different design configurations for cold plasma applications, analysis the mechanisms of microbial spore and biofilm inactivation, and examines the impact of cold plasma on food compositional, organoleptic, and nutritional quality.

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Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms

Cited by 23 publications

References 41 publications

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

A Cold Plasma Technology for Ensuring the Microbiological Safety and Quality of Foods

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