Detection and Quantification of Polychlorinated Biphenyl Sulfates in Human Serum

Zhang, Duo; Saktrakulkla, Panithi; Tuttle, Kristopher; Marek, Rachel F.; Lehmler, Hans‐Joachim; Wang, Kai; Hornbuckle, Keri C.; Duffel, Michael W.

doi:10.1021/acs.est.0c06983

Cited by 24 publications

(29 citation statements)

References 43 publications

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“…This contamination is related to production through modern manufacturing processes and leaching from old construction materials and hazardous waste sites [ 72 ]. PCB sulfates are derived from the metabolism of hydroxylated PCBs (OH-PCBs) [ 73 ]. PCBs can interact with steroid receptors or modulate their expression.…”

Section: Endocrine-disrupting Chemicals (Edcs) Transgenerational Impactmentioning

confidence: 99%

Impact of Endocrine Disruptors upon Non-Genetic Inheritance

Montjean

Neyroud

Yéfimova

et al. 2022

IJMS

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Similar to environmental factors, EDCs (endocrine-disrupting chemicals) can influence gene expression without modifying the DNA sequence. It is commonly accepted that the transgenerational inheritance of parentally acquired traits is conveyed by epigenetic alterations also known as “epimutations”. DNA methylation, acetylation, histone modification, RNA-mediated effects and extracellular vesicle effects are the mechanisms that have been described so far to be responsible for these epimutations. They may lead to the transgenerational inheritance of diverse phenotypes in the progeny when they occur in the germ cells of an affected individual. While EDC-induced health effects have dramatically increased over the past decade, limited effects on sperm epigenetics have been described. However, there has been a gain of interest in this issue in recent years. The gametes (sperm and oocyte) represent targets for EDCs and thus a route for environmentally induced changes over several generations. This review aims at providing an overview of the epigenetic mechanisms that might be implicated in this transgenerational inheritance.

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Section: Endocrine-disrupting Chemicals (Edcs) Transgenerational Impactmentioning

confidence: 99%

Impact of Endocrine Disruptors upon Non-Genetic Inheritance

Montjean

Neyroud

Yéfimova

et al. 2022

IJMS

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“…Diazomethane was prepared by the Synthesis Core of the Iowa Superfund Research Program (ISRP) as previously described. 31 …”

Section: Methodsmentioning

confidence: 99%

“…As seen in Table S1 , methoxy-PCB standards for GC-MS/MS quantitation were either prepared by the ISRP Synthesis Core or purchased from Wellington Laboratories (Guelph, ON), AccuStandard (New Haven, CT), CDN Isotopes (Pointe-Claire, QC) and Cambridge Isotope Laboratories (Tewksbury, MA), as indicated previously. 31 All solvents for extraction and analysis of OH-PCBs were of pesticide residue analysis grade.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction, separation, derivatization, and cleanup methods for OH-PCB quantification were carried out as described previously. 31 Briefly, samples after incubation with and without purified sulfatase were denatured with 6 N hydrochloric acid (HCl) and 2-propanol, and this was followed by extraction with 1:1 (v/v) hexane: methyl tert -butyl ether (MTBE). The extracts were washed with 1% (w/w) potassium chloride (KCl) and, then, subjected to liquid–liquid partitioning with potassium hydroxide and hexane.…”

Section: Methodsmentioning

confidence: 99%

“… 18 , 29 , 30 Our recent development of a procedure for analysis of multiple PCB sulfates in human serum has provided the methodology to investigate the prevalence of these sulfated PCB metabolites in the two populations that have been the subject of the AESOP Study. 31 We have now applied this method for extraction and quantification of PCB sulfates in human serum to samples collected during 2015–2016 from 48 individuals living in urban and rural communities within the AESOP Study. Our results provide new insights into the potential reasons for unresolved questions relating to exposures and serum concentrations of PCBs and OH-PCBs.…”

Section: Introductionmentioning

confidence: 99%

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PCB Sulfates in Serum from Mothers and Children in Urban and Rural U.S. Communities

Zhang

Saktrakulkla

Marek

et al. 2022

Environ. Sci. Technol.

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Serum samples from 24 subjects (6 mother–daughter and 6 mother–son dyads) in a rural community (Columbus Junction, Iowa) and 24 subjects (6 mother–daughter and 6 mother–son dyads) in an urban community (East Chicago, Indiana) were analyzed for 74 sulfated metabolites of polychlorinated biphenyls (PCBs). We detected significantly higher mean concentrations of total assessed PCB sulfates in the urban group (110–8900 ng/g fresh weight of serum, mean = 3400 ng/g, standard error = 300) than in the rural cohort (530–6700 ng/g fresh weight of serum, mean = 1800 ng/g, standard error = 500). Eight PCB sulfate congeners (4-PCB 2 sulfate, 4′-PCB 2 sulfate, 2′-PCB 3 sulfate, 4′-PCB 3 sulfate, 4-PCB 11 sulfate, 4′-PCB 18 sulfate, 4′-PCB 25 sulfate, and 4-PCB 52 sulfate) contributed over 90% of the total assessed PCB sulfates in most individuals. The serum samples were enriched in PCB sulfates with fewer than 5 chlorine atoms, and this congener distribution differed from those of PCBs and hydroxylated PCBs in previous studies in the same communities. Regression analysis indicated several significant congener-specific correlations in mother–child dyads, and these relationships differed by location and by mother–daughter or mother–son dyads. This is the first study reporting a broad range of PCB sulfates in populations from urban and rural areas.

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