Identification of two highly antigenic epitope markers predicting multiple sclerosis in optic neuritis patients

Sadam, Helle; Pihlak, Arno; Jaago, Mariliis; Pupina, Nadežda; Rähni, Annika; Toots, Maarja; Vaheri, Antti; Nieminen, Janne; Siuko, Mika; Tienari, Pentti J.; Palm, Kaia

doi:10.1016/j.ebiom.2021.103211

Cited by 14 publications

(21 citation statements)

References 23 publications

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“…Previous studies have illustrated that CMV or EBV infection may lead to poor prognosis of IIDD and increase the risk of death. Sadam et al 108 reported that patients with NMO who had CMV infection were at a high risk of progressing to MS; thus, CMV viremia could lead to poor prognosis. Similarly, Lünemann et al 109 found that immune reactions to EBV‐encoded nuclear antigen‐1 (EBNA1) could trigger clinically isolated syndromes to convert to MS and suggested that EBNA1‐specific IgG could be used as a prognostic marker for disease progression.…”

Section: Discussionmentioning

confidence: 99%

Clinical risk factors and prognostic model for idiopathic inflammatory demyelinating diseases after haploidentical hematopoietic stem cell transplantation in patients with hematological malignancies

Deng

et al. 2021

American J Hematol

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Idiopathic inflammatory demyelinating diseases (IIDDs) of the central nervous system (CNS) are rare but serious neurological complications of haploidentical hematopoietic stem cell transplantation (haplo‐HSCT). However, the risk factors and a method to predict the prognosis of post‐transplantation CNS IIDDs are not available. This retrospective study first reviewed data from 4532 patients who received haplo‐HSCT during 2008–2019 in our center, and 184 patients (4.1%) with IIDDs after haplo‐HSCT were identified. Grades II to IV acute graft‐versus‐host disease (aGVHD) (p < 0.001) and chronic GVHD (cGVHD) (p = 0.009) were identified as risk factors for developing IIDDs after haplo‐HSCT. We then divided the 184 IIDD patients into a derivation cohort and validation cohort due to transplantation time to develop and validate a model for predicting the prognosis of IIDDs. In the multivariate analysis of the derivation cohort, four candidate predictors were entered into the final prognostic model: cytomegalovirus (CMV) infection, Epstein–Barr virus (EBV) infection, IgG synthesis (IgG‐syn) and spinal cord lesions. The prognostic model had an area under the receiver operating characteristic curve of 0.864 (95% CI: 0.803–0.925) in the internal validation cohort and 0.871 (95% CI: 0.806–0.931) in the external validation cohort. The calibration plots showed a high agreement between the predicted and observed outcomes. Decision curve analysis indicated that IIDD patients could benefit from the clinical application of the prognostic model. The identification of IIDD patients after allo‐HSCT who have a poor prognosis might allow timely treatment and improve patient survival and outcomes.

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Section: Discussionmentioning

confidence: 99%

Clinical risk factors and prognostic model for idiopathic inflammatory demyelinating diseases after haploidentical hematopoietic stem cell transplantation in patients with hematological malignancies

Deng

et al. 2021

American J Hematol

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“…The CVD group included samples from patients with chronic coronary artery disease (CAD, n = 32) and with acute coronary syndrome (ACS, n = 32) who were diagnosed by coronary angiography, cardiac enzyme and symptomatic findings 30 (Table S4). The control group (CTRL, n = 402) included samples from individuals with no known history of CAD, ACS or MI and who were either healthy ( n = 125) or had different primary diagnoses ( n = 277) (including gum disease ( n = 25), 30 breast cancer ( n = 57), 31 myasthenia gravis ( n = 129), multiple sclerosis ( n = 20), 32 narcolepsy ( n = 16), 33 or schizophrenia ( n = 30)).…”

Section: Methodsmentioning

confidence: 99%

“…All study samples were analysed using the mimotope-variation analysis method (MVA, 32 , 33 ). In brief, modified random 12-mer peptide phage library (originally derived from Ph.D.-12, New England Biolabs) was amplified according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

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Immune response to a conserved enteroviral epitope of the major capsid VP1 protein is associated with lower risk of cardiovascular disease

et al. 2022

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Background Major cardiac events including myocardial infarction (MI) are associated with viral infections. However, how specific infections contribute to the cardiovascular insults has remained largely unclear.Methods We employed next generation phage display mimotope-variation analysis (MVA) to explore the link between antibody-based immune response and severe cardiovascular conditions. Here, we used a case-control design, including the first-stage discovery cohort (n = 100), along with cohorts for second-stage discovery (n = 329) and validation (n = 466).Findings We observed strong antibody response to the peptide antigens with Gly-Ile-X-Asp (G-I-X-D) core structure in healthy individuals but not in patients with MI. Analysis of the origin of this epitope linked it with the N-terminus of the VP1 protein of poliovirus 3 (PV3), but also other species of picornaviruses. Consistently, we found low levels of antibody response to the G-I-X-D epitope in individuals with severe cardiac disease complications.Interpretation Our findings imply that antibody response to the G-I-X-D epitope is associated with polio vaccinations and that high antibody levels to this epitope could discriminate healthy individuals from prospective MI patients as a blood-derived biomarker. Together, these findings highlight the importance of epitope-specific antibody response and suggest that protective immunity against the polio-and non-polio enteroviral infections support improved cardiovascular health.

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“…With another method AMIDA for example, immunoprecipitation is used for separation with subsequent MS analysis [ 90 ]. This is often validated by microarray analyses in which recombinant antigens are incubated with serum, but also using phage displays to detect AAB reactivities against synthesized proteins are prominent [ 91 ]. The first up-regulated AABs identified in the past were AABs against heat shock proteins [ 92 ], gamma-enolase [ 93 ], alpha-fodrin [ 94 ], and myelin basic protein [ 95 ].…”

Section: Diagnosis Of Glaucoma and Screening For Potential Biomarkersmentioning

confidence: 99%

Antibody and Protein Profiles in Glaucoma: Screening of Biomarkers and Identification of Signaling Pathways

Auler

Tonner

Pfeiffer

et al. 2021

Biology

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Glaucoma represents a group of chronic neurodegenerative diseases, constituting the second leading cause of blindness worldwide. To date, chronically elevated intraocular pressure has been identified as the main risk factor and the only treatable symptom. However, there is increasing evidence in the recent literature that IOP-independent molecular mechanisms also play an important role in the progression of the disease. In recent years, it has become increasingly clear that glaucoma has an autoimmune component. The main focus nowadays is elucidating glaucoma pathogenesis, finding early diagnostic options and new therapeutic approaches. This review article summarizes the impact of different antibodies and proteins associated with glaucoma that can be detected for example by microarray and mass spectrometric analyzes, which (i) provide information about expression profiles and associated molecular signaling pathways, (ii) can possibly be used as a diagnostic tool in future and, (iii) can identify possible targets for therapeutic approaches.

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Identification of two highly antigenic epitope markers predicting multiple sclerosis in optic neuritis patients

Cited by 14 publications

References 23 publications

Clinical risk factors and prognostic model for idiopathic inflammatory demyelinating diseases after haploidentical hematopoietic stem cell transplantation in patients with hematological malignancies

Clinical risk factors and prognostic model for idiopathic inflammatory demyelinating diseases after haploidentical hematopoietic stem cell transplantation in patients with hematological malignancies

Immune response to a conserved enteroviral epitope of the major capsid VP1 protein is associated with lower risk of cardiovascular disease

Antibody and Protein Profiles in Glaucoma: Screening of Biomarkers and Identification of Signaling Pathways

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