Flow control in a laminate capillary-driven microfluidic device

Jang, Inseok; Kang, Hak-Hee; Song, Simon; Dandy, David S.; Geiss, Brian J.; Henry, Charles S.

doi:10.1039/d0an02279a

Cited by 41 publications

(51 citation statements)

References 43 publications

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“…With this valve, the fluids enter the main channel at the same time to ensure mixing. [33] Once this happens, the three fluids (water, Tf and Os (VI) complex solutions) flow to the main channel outlet. At this point, the labeling reaction began (TfÀ Os (VI) adduct formation), so an incubation period was required.…”

Section: Resultsmentioning

confidence: 99%

“…[9,28,29] One promising approach is the use of capillary-driven microfluidics, also named passive methods. In these systems, fluids autonomously move along a channel without external force by the capillary effect, [30][31][32][33] unlike traditional microfluidic devices, which use external forces such as pressure, magnetic, electrical, etc. [34,35] Capillary-driven flow is quite robust and easy to use, and does not require any moving components or external power.…”

Section: Introductionmentioning

confidence: 99%

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Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

et al. 2021

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A disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking of polyethylene terephthalate (PET) films and double adhesive layers, it is possible to create a geometry that allows the filling of the tailored channels in a fixed time. In the main channel of this passive microfluidic device, the necessary steps to perform the electrochemical determination of transferrin are performed: labeling of the protein with Os (VI) complex (electrochemical tag), washing with water of the osmium residues and electro-chemical detection by adsorptive transfer stripping square wave voltammetry. Electrochemical detection released two electrochemical signals: one from Os (VI) complex due to carbohydrates at À 0.9 V, and other from the intrinsic electrochemical signal of glycoprotein due to the amino acids at + 0.8 V. The ratio between them establishes an indicator of the degree of glycosylation (called electrochemical index of glycosylation). The method was successfully applied to the analysis of clinical samples from patients with congenital disorders of glycosylation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

et al. 2021

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“…Due to their improved flow capacities and limited nonspecific adsorption, laminated devices offer better analytical performance than paper-based devices. 33 …”

Section: Introductionmentioning

confidence: 99%

Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care

et al. 2021

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Rapid and inexpensive serological tests for SARS-CoV-2 antibodies are needed to conduct population-level seroprevalence surveillance studies and can improve diagnostic reliability when used in combination with viral tests. Here, we report a novel low-cost electrochemical capillary-flow device to quantify IgG antibodies targeting SARS-CoV-2 nucleocapsid proteins (anti-N antibody) down to 5 ng/mL in low-volume (10 μL) human whole blood samples in under 20 min. No sample preparation is needed as the device integrates a blood-filtration membrane for on-board plasma extraction. The device is made of stacked layers of a hydrophilic polyester and double-sided adhesive films, which create a passive microfluidic circuit that automates the steps of an enzyme-linked immunosorbent assay (ELISA). The sample and reagents are sequentially delivered to a nitrocellulose membrane that is modified with a recombinant SARS-CoV-2 nucleocapsid protein. When present in the sample, anti-N antibodies are captured on the nitrocellulose membrane and detected via chronoamperometry performed on a screen-printed carbon electrode. As a result of this quantitative electrochemical readout, no result interpretation is required, making the device ideal for point-of-care (POC) use by non-trained users. Moreover, we show that the device can be coupled to a near-field communication potentiostat operated from a smartphone, confirming its true POC potential. The novelty of this work resides in the integration of sensitive electrochemical detection with capillary-flow immunoassay, providing accuracy at the point of care. This novel electrochemical capillary-flow device has the potential to aid the diagnosis of infectious diseases at the point of care.

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“…Although passive microfluidics can work without the use of external field generators, the operation of most of these devices still relies heavily on bulk external fluid-driven systems (such as syringe pumps, peristaltic pumps, and gas-driven fluid pumping systems), which require electricity as the power source and are difficult to miniaturize. To address this limitation, various on-chip fluid pumping systems using capillary force [ 59 ], surface energy gradient [ 60 ], electroosmotic flow [ 61 ], and acoustic streaming [ 62 ] have been explored. However, the flow rates provided by these pumping systems are very low and thus are incapable of driving flows in high-flow-rate systems (such as inertial microfluidics).…”

Section: Introductionmentioning

confidence: 99%

Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge

Xiang

Zhang

2021

Biosensors

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Conventional sample preparation techniques require bulky and expensive instruments and are not compatible with next-generation point-of-care diagnostic testing. Here, we report a manually operated syringe-tip inertial microfluidic centrifuge (named i-centrifuge) for high-flow-rate (up to 16 mL/min) cell concentration and experimentally demonstrate its working mechanism and performance. Low-cost polymer films and double-sided tape were used through a rapid nonclean-room process of laser cutting and lamination bonding to construct the key components of the i-centrifuge, which consists of a syringe-tip flow stabilizer and a four-channel paralleled inertial microfluidic concentrator. The unstable liquid flow generated by the manual syringe was regulated and stabilized with the flow stabilizer to power inertial focusing in a four-channel paralleled concentrator. Finally, we successfully used our i-centrifuge for manually operated cell concentration. This i-centrifuge offers the advantages of low device cost, simple hand-powered operation, high-flow-rate processing, and portable device volume. Therefore, it holds potential as a low-cost, portable sample preparation tool for point-of-care diagnostic testing.

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Flow control in a laminate capillary-driven microfluidic device

Abstract: We present novel flow control methods including valve, mixing control, and flow rate control applicable to the laminate capillary-driven microfluidic devices.

Cited by 41 publications

References 43 publications

Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care

Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge

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