Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge

Xiang, Nan; Zhang, Ni

doi:10.3390/bios12010014

Cited by 6 publications

(6 citation statements)

References 68 publications

(75 reference statements)

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“…Correspondingly, the sample throughput of the planar spiral centrifuge is much bigger. Xiang and Ni [11] even included a centrifuge with multi-layer parallel microchannels designed to increase the sample processing capacity. Of course, this also means that the design and manufacture of the planar spiral centrifuge is also relatively difficult.…”

Section: Resultsmentioning

confidence: 99%

“…To increase the sample processing capacity, Xiang et al [11] reported a manually operated inertial microfluidic centrifuge with a four-channel parallel structure, which has the advantages of simple operation and high flow processing. However, this microchannel has a disc-like helical structure; the inner ring channel radius is small, and the outer channel radius is large, which means the inertial lift force is not constant.…”

Section: Introductionmentioning

confidence: 99%

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Mixed particles separation based on a cylindrical microfluidic centrifuge

Su,

Miao

2024

J. Micromech. Microeng.

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This paper presents a novel cylindrical centrifuge designed to separate microspheres and cells. The polyvinylidene-fluoride tubes with heat shrink characteristics were used to encapsulate helix microchannels. Numerical simulations were employed to analyze and design the channel helix structure (with five annular loops, 2 mm pitch and 20 mm cylinder diameter). Then, The inertial focusing of microspheres (with diameters of 10, 15 and 20 µm) and the freshwater microalgae (i.e. Haematococcus pluvialis) in the cylindrical centrifuge were studied experimentally. As the particle size increased, the focusing position moved toward the center line of the flow channel, and the focus degree tended to decrease. When the flow rate increased, the focus position barely changed, but the focus degree increased significantly. A quantitative study of the centrifuge efficiency revealed that when the initial concentration was 104 particles ml−1 and the flow rate was at its optimal 1.7 ml min−1, the centrifugal efficiency (CE) values of 10 µm, 15 µm, 20 µm microspheres, and H. pluvialis, were 98.8%, 87.8%, 70.8% and 64.6%, respectively. The CE is inversely proportional to the microsphere size and the initial concentrations. Compared with the other two centrifuges (the cavity-vortex and planar spiral), the cylindrical centrifuge design and manufacturing process have a simplicity that provides low cost, efficient sample handling, and effective separation of microspheres and biological cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mixed particles separation based on a cylindrical microfluidic centrifuge

Su,

Miao

2024

J. Micromech. Microeng.

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“…Xiang et al reported a hand-powered inertial microfluidic syringe tip centrifuge with a high flow rate of 16 mL min −1 . [213] This offers a potential solution to the traditionally large centrifuges that are often needed for sample management alongside biosensing devices. The centrifuge consists of a syringe tip flow stabilizer and a syringe filter-like inertial microfluidic concentrator.…”

Section: Sample Preparation and Manual Handlingmentioning

confidence: 99%

Toward Personalized Nanomedicine: The Critical Evaluation of Micro and Nanodevices for Cancer Biomarker Analysis in Liquid Biopsy

Clack

Soda

Kasetsirikul

et al. 2023

Small

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from circulating biomarkers (CBs). CBs may be found in several minimally invasive bodily fluids including (but not limited to) blood, saliva, urine, pleural effusions, stool, and cerebrospinal fluid. CBs may include circulating tumor cells (CTCs), circulating tumor RNA/DNA (ctRNA/ctDNA), microRNAs (miRNAs), long non-coding RNAs (lncRNAs), proteins, exosomes, autoantibodies, all of which, are known for their diagnostic and prognostic potential. [1] Currently, clinicians and centralized hospitals practice tissue biopsy for diagnosis and characterization of the heterogeneity of the solid tumor which is crucial for personalized medicine. [1a] Despite the many decades of clinical practice, tissue-based biopsies are subject to sampling bias, suffer from the issue of low repeatability, and cause immense pain in patients as the process is invasive. One of the major concerns of biopsies, often ignored, is the entrance of tumor cells into the systemic circulation during the tissue-sampling process, leading to tumor metastasis. [2] To overcome these issues, the detection of CBs through liquid biopsy has shown promise as a potential replacement due to the rapid and semi or non-invasive sampling process and the repeated analysis of samples during therapeutic interventions aid in decision-making about a particular patient's progress.Due to the advancement of highly specific sequencing and gene amplification technologies, liquid biopsies can access more relevant cancer biomarkers, providing a much-needed alternative to tissue-based biopsy. [1b] These recent advances have led to the development of many miniaturized/ portable platforms. The clinical potential of these micro and nano devices is rapidly growing, but they face many challenges before transitioning to point-of-care (POC) platforms that are sensitive, rapid, and accurate, as discussed herein.Previously, our group [3] provided a critical review of circulating tumor DNA (ctDNA) and liquid biopsy opportunities, challenges, and recent advances in detection technologies. The review examines the potential of ctDNA as a marker in liquid biopsy, as well as the detection methods, challenges, and considerations. We further [1b] provided a comprehensive review of advanced liquid biopsy technologies for CBs detection. Others Liquid biopsy for the analysis of circulating cancer biomarkers (CBs) is a major advancement toward the early detection of cancer. In comparison to tissue biopsy techniques, liquid biopsy is relatively painless, offering multiple sampling opportunities across easily accessible bodily fluids such as blood, urine, and saliva. Liquid biopsy is also relatively inexpensive and simple, avoiding the requirement for specialized laboratory equipment or trained medical staff. Major advances in the field of liquid biopsy are attributed largely to developments in nanotechnology and microfabrication that enables the creation of highly precise chip-based platforms. These devices can overcome detection limitations of an individual biomarker by detecting multiple markers ...

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“…A prominent advantage of inertial microfluidics is that it can be operated by simply driving the flow within a specific flow-rate range. Therefore, the mechanical force provided by the compression spring 154 or the power generated by manually pushing the syringe 70,[155][156][157][158] can be used to drive the sample flow in inertial microfluidics, which enables electricity-free operation for use in low-resource settings. We believe that a growing number of inertial microfluidics-based instruments will be developed in the future for widespread biomedical applications.…”

Section: Commercial Instruments For Practical Applicationsmentioning

confidence: 99%