The ultrafiltration molecular weight cut-off has a limited effect on the concentration and protein profile during preparation of human milk protein concentrates

Sergius-Ronot, Mélanie; Suwal, Shyam; Shama, Sara; Chamberland, Julien; Unger, Sharon; O'Connor, Deborah L; Pouliot, Yves; Doyen, Alain

doi:10.3168/jds.2020-18762

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…UF/DF performance was assessed following the protocol of Sergius-Ronot et al. with modifications . The permeate flux ( J ; L/h per m 2 ) was monitored every 15 min throughout the experiments and calculated according to eq : J = V p A where V p is the volume (L) of the permeate collected over 30 s and A is the active membrane filtration area (m 2 ).…”

Section: Methodsmentioning

confidence: 99%

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Combination of Ultrafiltration/Diafiltration and Ammonium Sulfate Precipitation for the Purification of 11S and 7S Pea Globulin Fractions

Gravel,

Dubois-Laurin,

Turgeon

et al. 2023

ACS Food Sci. Technol.

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Section: Methodsmentioning

confidence: 99%

“…UF/DF performance was assessed following the protocol of Sergius-Ronot et al with modifications. 12 The permeate flux (J; L/h per m 2 ) was monitored every 15 min throughout the experiments and calculated according to eq 1:…”

Section: Introductionmentioning

confidence: 99%

Combination of Ultrafiltration/Diafiltration and Ammonium Sulfate Precipitation for the Purification of 11S and 7S Pea Globulin Fractions

Gravel,

Dubois-Laurin,

Turgeon

et al. 2023

ACS Food Sci. Technol.

Self Cite

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“…Various methods and their combination have been used for the separation/isolation of milk proteins and the peptides derived from them either in laboratory setup or on industrial scale. Among them, membrane separation, solvent extraction, and centrifugation have been applied abundantly [84,85] as well as various affinity enrichment techniques have been utilized [86][87][88]. As well, to simplify the complex protein mixture in milk, casein fraction is often separated from whey proteins, for example, by isoelectric precipitation of casein at pH 4.5-4.6 using various acids followed by centrifugation and filtration steps.…”

Section: Recent Advances In Separation Of Milk Bioactive Proteins/pep...mentioning

confidence: 99%

Advances in separation and identification of biologically important milk proteins and peptides

Št́astná

2023

Electrophoresis

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Milk is a rich source of biologically important proteins and peptides. In addition, milk contains a variety of extracellular vesicles (EVs), including exosomes, that carry their own proteome cargo. EVs are essential for cell–cell communication and modulation of biological processes. They act as nature carriers of bioactive proteins/peptides in targeted delivery during various physiological and pathological conditions. Identification of the proteins and protein‐derived peptides in milk and EVs and recognition of their biological activities and functions had a tremendous impact on food industry, medicine research, and clinical applications. Advanced separation methods, mass spectrometry (MS)‐based proteomic approaches and innovative biostatistical procedures allowed for characterization of milk protein isoforms, genetic/splice variants, posttranslational modifications and their key roles, and contributed to novel discoveries. This review article discusses recently published developments in separation and identification of bioactive proteins/peptides from milk and milk EVs, including MS‐based proteomic approaches.

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“…Not all of these proteins are digested and excreted outside the body. Biologically active proteins have many advantages for breast milk, as they can have enzymatic activity, enhance the absorption of nutrients, and stimulate growth [5], studied the amount of protein entering the infant's body through breastfeeding They found that protein provides a good amount of essential amino acids that the child needs for growth and that the change in protein levels varies according to the period of breastfeeding, as its percentage in colostrum is higher than in transitional milk. It begins to decrease after the first six months of breastfeeding.…”

Section: Introductionmentioning

confidence: 99%

The Effect of High-Protein Biscuits Eaten by Breastfeeding Women

Weshah,

Rahi,

Mustafa

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Breast milk is considered the ideal food for an infant in the first six months, as it contains water, fats, proteins, lactose, minerals, vitamins, enzymes, and hormones. Breast milk varies greatly in composition between breastfeeding mothers and even within the same mother from time to time. Milk samples were collected from breastfeeding women, and the number of donor women was 8 women per group. Breastfeeding women were divided into groups (A, B, C). Group A is the control group that did not take a nutritional supplement or high-protein biscuit Group B is for donor women who take nutritional supplements, and Group C is for breastfeeding women who eat high-protein biscuits. Tests were conducted on biscuits and human milk. The results of the analysis for the biscuits showed an increase in the percentage of moisture, protein, and ash in L3, while the percentage of fat and carbohydrates recorded an increase in the standard treatment. When conducting a sensory evaluation of the biscuits, a difference was noted in the sensory evaluation characteristics between the treatments. After the breastfeeding mother ate protein-rich biscuits, the results of the chemical analysis of the milk showed an increase in the moisture percentage, while the percentages of fat, protein ML3, and lactose increased. It was high in treatment ML1 and ML2, while the ash percentage recorded the highest value in treatment ML1. When analyzing the amino acids in milk samples, the amino acids varied in their proportions, and this indicates the effect of protein-rich foods on the micro- and macronutrients in human milk.

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The ultrafiltration molecular weight cut-off has a limited effect on the concentration and protein profile during preparation of human milk protein concentrates

Cited by 10 publications

References 44 publications

Combination of Ultrafiltration/Diafiltration and Ammonium Sulfate Precipitation for the Purification of 11S and 7S Pea Globulin Fractions

Combination of Ultrafiltration/Diafiltration and Ammonium Sulfate Precipitation for the Purification of 11S and 7S Pea Globulin Fractions

Advances in separation and identification of biologically important milk proteins and peptides

The Effect of High-Protein Biscuits Eaten by Breastfeeding Women

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