Short-term drought triggers defence mechanisms faster than ABA accumulation in the epiphytic bromeliad Acanthostachys strobilacea

Carvalho, Victória; Gaspar, Marı́lia; Nievola, Catarina Carvalho

doi:10.1016/j.plaphy.2020.12.030

Cited by 8 publications

(12 citation statements)

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“…Relative water content (RWC) and osmotic potential were analysed from fresh leaf disks 0.5 cm in diameter, following Fleta-Soriano et al (2015) and Rigui et al (2019), respectively. SWC was assessed from aliquots obtained from three separate trays of each treatment, following Carvalho et al (2021). CAM activity was assessed as the titratable acidity of samples obtained at dawn and dusk, according to Menezes et al (2020).…”

Section: Biometric Water Status Cam and Chlorophyll Fluorescence Meas...mentioning

confidence: 99%

“…The activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) were quantified following Carvalho et al (2017). Total glutathione, reduced (GSH) and oxidized (GSSG) fractions were quantified with the enzymatic recycling assay as described in Carvalho et al (2021). The glutathione redox state was inferred by calculating percentage of the GSH pool.…”

Section: Free Amino Acid (Aa) Contentmentioning

confidence: 99%

“…2020; Carvalho et al . 2021). In this study, we tested the hypothesis that juvenile A. strobilacea plants exhibit improved biochemical and physiological responses to a secondary event of drought and rewatering compared to a single cycle, as indicative of stress memory.…”

Section: Introductionmentioning

confidence: 99%

“…We evaluated parameters associated with CAM activity, photosynthetic integrity, nitrosative metabolism, antioxidant system and osmotic adjustment, since previous research evidenced that these mechanisms are part of short‐term drought defence in juvenile A. strobilacea (Carvalho et al . 2021) and/or in the drought memory of other species (Alves et al . 2020; Lukić et al .…”

Section: Introductionmentioning

confidence: 99%

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Drought memory in Acanthostachys strobilacea, a CAM epiphytic bromeliad

Carvalho,

Gaspar,

Nievola

2024

Plant Biol J

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Stress memory is the development of altered responses to stress due to previous exposure, which might result in increased tolerance. Biochemical and physiological parameters shown to be positively affected by stress memory include those of the antioxidant and nitrosative metabolism, photosynthetic pigments and osmolyte content. Epiphytic bromeliads likely present stress memory since they experience frequent droughts in the canopies. Thus, we aimed to evaluate if the epiphytic bromeliad Acanthostachys strobilacea (Schult. & Schult.f.) Klotzsch shows improved metabolic stress defence responses to a second drought and rewatering cycle compared to a single exposure. In a controlled environment chamber, 90‐day‐old plants were exposed to one or two drought–rewatering cycles of 14 days without irrigation and 5 days of rewatering each. Sampling occurred after the final drought and rewatering periods for one or two cycles treatments. The free amino acid, chlorophyll, and carotenoid levels and S‐nitrosoglutathione reductase (GSNOR) activity were higher at the second drought than at the first exposure. The rise in nocturnal acidification (indicative of increased CAM activity) caused by the initial drought persisted through the second drought–rewatering cycle, implying a lasting memory effect on CAM activity. Furthermore, the second recovery did not induce glutathione accumulation, as in the first rewatering event, suggesting the pre‐exposure to drought reduced this thiol's demand during a later recovery. Our results evidence metabolic changes related to drought stress memory in A. strobilacea, supporting this mechanism might be involved in the tolerance of epiphytic bromeliads to intermittent droughts.

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Section: Biometric Water Status Cam and Chlorophyll Fluorescence Meas...mentioning

confidence: 99%

Section: Free Amino Acid (Aa) Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Drought memory in Acanthostachys strobilacea, a CAM epiphytic bromeliad

Carvalho,

Gaspar,

Nievola

2024

Plant Biol J

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“…Drought tolerance is the capacity to resist dehydration via physiological and molecular activity. Among the fastest physiological processes induced by drought is abscisic acid (ABA)-mediated stomatal closure, which promotes water conservation and activates expression of stress response genes (Carvalho et al 2021). Further acclimation reactions under prolonged drought stress or increased stress intensity include osmotic adjustment, reprogramming of metabolism, cell wall modifications and activation of the antioxidant system (Laxa et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Intrinsic morphology and spatial distribution of non‐structural carbohydrates contribute to drought resistance of two mulberry cultivars

et al. 2023

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Drought is one of the most adverse environmental stresses limiting plant growth and productivity. However, the underlying mechanisms regarding metabolism of nonstructural carbohydrates (NSC) in source and sink organs are still not fully elucidated in woody trees.• Saplings of mulberry cv Zhongshen1 and Wubu were subjected to a 15-day progressive drought stress. NSC levels and gene expression involved in NSC metabolism were investigated in roots and leaves. Growth performance and photosynthesis, leaf stomatal morphology, and other physiological parameters were also analysed.• Under well-watered conditions, Wubu had a higher R/S, with higher NSC in leaves than in roots; Zhongshen1 had a lower R/S with higher NSC in roots than leaves. Under drought stress, Zhongshen1 showed decreased productivity and increased proline, abscisic acid, ROS content and activity of antioxidant enzymes, while Wubu sustained comparable productivity and photosynthesis. Interestingly, drought resulted in decreased starch and slightly increased soluble sugars in leaves of Wubu, accompanied by notable downregulation of starch-synthesizing genes and upregulation of starchdegrading genes. Similar patterns in NSC levels and relevant gene expression were also observed in roots of Zhongshen1. Concurrently, soluble sugars decreased and starch was unchanged in roots of Wubu and leaves of Zhongshen1. However, gene expression of starch metabolism in roots of Wubu was unaltered, but in leaves of Zhongshen1 starch metabolism was more activated.• These findings revealed that intrinsic R/S and spatial distribution of NSC in roots and leaves concomitantly contribute to drought resistance in mulberry.

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Tank formation transforms nitrogen metabolism of an epiphytic bromeliad and its phyllosphere bacteria

Stryker,

White,

Díaz‐Almeyda

et al. 2024

American J of Botany

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PremiseUp to half of tropical forest plant species grow on other plants. Lacking access to soils, vascular epiphytes have unique adaptations for mineral nutrition. Among the most distinctive is the tank growth form of certain large bromeliads, which absorb nutrients that are cycled by complex microbial communities in water trapped among their overlapping leaf bases. However, tanks form only after years of growth by juvenile plants, which must acquire nutrients differently. Understanding how nutrient dynamics change during tank bromeliad development can provide key insights into the role of microorganisms in the maintenance of tropical forest biodiversity.MethodsWe evaluated variations in plant morphology, growth, foliar nitrogen physiology, and phyllosphere bacterial communities along a size gradient spanning the transition to tank formation in the threatened species Tillandsia utriculata.ResultsSequential morphological and growth phases coincided with the transition to tank formation when the longest leaf on plants was between 14 and 19 cm. Before this point, foliar ammonium concentrations were very high, but after, leaf segments absorbed significantly more nitrate. Leaf‐surface bacterial communities tracked ontogenetic changes in plant morphology and nitrogen metabolism, with less‐diverse communities in tankless plants distinguished by a high proportion of taxa implicated in ureolysis, nitrogen fixation, and methanotrophy, whereas nitrate reduction characterized communities on individuals that could form a tank.ConclusionsCoupled changes in plant morphology, physiology, and microbiome function facilitate the transition between alternative nutritional modes in tank bromeliads. Comparing bromeliads across life stages and habitats may illuminate how nitrogen‐use varies across scales.

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Short-term drought triggers defence mechanisms faster than ABA accumulation in the epiphytic bromeliad Acanthostachys strobilacea

Cited by 8 publications

References 78 publications

Drought memory in Acanthostachys strobilacea, a CAM epiphytic bromeliad

Drought memory in Acanthostachys strobilacea, a CAM epiphytic bromeliad

Intrinsic morphology and spatial distribution of non‐structural carbohydrates contribute to drought resistance of two mulberry cultivars

Tank formation transforms nitrogen metabolism of an epiphytic bromeliad and its phyllosphere bacteria

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