Simultaneous determination of six glycosidic aroma precursors in pomelo by ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

A rapid, accurate and efficient UPLC-MS/MS method was established for the simultaneous determination of six glycosidic aroma precursors in pomelo.

Determination of Tea Aroma Precursor Glycosides: An Efficient Approach via Liquid Chromatography-Tandem Mass Spectrometry

“…The sensitivity (peak area) of different ion transitions that were obtained by both positive and negative ESI modes was further compared with the injected standard glycosides (Figure ). Interestingly, the product ions of Ger-glc that were not observed in the previous study were successfully optimized in this study (Figure G) . The optimal ion transition of Ben-glc, Ben-pri, 2pe-glc, 2pe-pri, Hex-glc, Hex-pri, Ger-glc, and Ger-pri, was 269.2 > 70.9, 447.1 > 268.8, 307.1 > 245.6, 461.0 > 415.2, 339.0 > 203.0, 493.0 > 149.3, 307.0 > 89.0, and 393.3 > 89.0, respectively.…”

“…The MRM acquisition mode of HPLC-MS/MS enables simultaneous identification and quantitation of multiple components by monitoring their transitions from precursor ions to dominant product ions . The sensitivity of the MRM mode is highly dependent upon the parent ions, product ions, tuning of the fragmentor voltage, and collision energy (CE) .…”

“…A suitable LC separation method can rapidly identify glycosides and distinguish the analytes that have the same glycosyl group and similar aglycone . For example, an efficient UPLC-MS/MS method has been established to identify six aroma precursor glycosides in pomelo simultaneously within 16 min.…”

“…GC-MS has also detected the derivatives of aroma precursor glycosides with trifluoroacetyl conversion. , The quality of ion-scanning monitoring may be difficult due to the nonselective derivatization of the aromatic precursor of glycosides. Several recent studies have focused on the direct analysis of the glycosides using liquid chromatography mass spectrometry (LC-MS) and LC-MS/MS instead of GC-MS in plants. − Detection methods have also evolved from primary mass spectrometry, such as full scan mode (SCAN) and single electron mode (SIM), to multistage mass spectrometry, such as multiple reaction monitoring (MRM) scan mode. ,, For example, six aroma precursor glycosides were detected simultaneously in pomelo using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) . However, the quantitative analysis of aroma precursor glycosides in the tea plant or the final product were mainly based on LC-MS, which is time-consuming and inefficient. , In addition, similar structures and the same MS fragment ions of glycosidic aroma precursors are both challenges for the simultaneous determination by LC-MS .…”

Visualizing the distribution of curcumin in the root of Curcuma longa via VUV-postionization mass spectrometric imaging

“…It is known that time-consuming and tedious sample pretreatment processes, including extraction, centrifugation, and concentration, 8,9 are required in detecting medical ingredients in herbal plants for traditional methods, such as gas chromatography-mass spectrometry (GC-MS) 10 and liquid chromatography-mass spectrometry (LC-MS). 11 Although the quantitative analysis of endogenous components in plant tissues can be obtained by these two methods, their pre-treatment destroys the integrity of plant tissues and makes them unable to provide more intuitive information about plant tissues, such as the initial distribution of target molecules in the plant tissues. In order to better understand the metabolism and pharmacological effects of herbal medicine, in situ detection of active ingredients in plant tissues is particularly important.…”