Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies

Abstract

Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids.

Cytotoxicity and Antiviral Properties of Alkaloids Isolated from Pancratium maritimum

“…These alkaloids were identified comparing their physical (specific optical rotation) and spectroscopic ( 1 H and 13 C NMR and ESI MS) properties with those already reported in the literature: for 1 by Lamoral-Theys et al (2009) [13] isolated from Stenbergia lutea, for 2 with those reported by Antoun et al (1993) [41] and Boit and Döpke (1960) [42] when isolated from Hymenocallis expansa and Hippeastrum aulicum, respectively. Alkaloids As previously reported, haemanthidine (7) was isolated as two 6-epimers [56] in a scalar ratio. The equilibrium between the two epimers was also reported for 6-hydroxycrinamine, but not for closely related crinine-type alkaloids such as 6α-hydroxycrinine and 6αhydroxybuphonisine.…”

Test Of Various Bulbil Sizes And Dolomite Dosages On Porang (Amorphophallus muelleri Blume.) Growth In Peat Media

“…This result is in line with Sumarwoto's (2004) research that large bulbils have more food reserves to support the faster growth of porang plants. The results of research by Zaragoza-Puchol et al (2021) showed that whole bulbils in various sizes and pieces gave a high germination value (>90%), except for small bulbils, which gave a low percentage of germination (±60%).…”

Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae)

“…The Amaryllidaceae family is known for its ornamental potential and the ability to synthesize several alkaloids, including galantamine and lycorine, and potent acetylcholinesterase (AChE) inhibitors, used to alleviate the symptoms of Alzheimer’s Disease (AD) [ 4 ]. However, some species in this family are poorly represented, have a low multiplication rate [ 5 ] and are vulnerable or at risk of extinction [ 6 ]. Among the species in this family, Zephyranthes irwiniana (Ravenna) Nic.…”