Study on the protective effect and mechanism of Dicliptera chinensis (L.) Juss (Acanthaceae) polysaccharide on immune liver injury induced by LPS

Xu, Qiongmei; Xu, Jie; Zhang, Kefeng; Zhong, Mingli; Cao, Houkang; Wei, Riming; Jin, Ling; Gao, Ya

doi:10.1016/j.biopha.2020.111159

Cited by 16 publications

(15 citation statements)

References 37 publications

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“…According to the investigation by World Health Organization (WHO), ALT serves as the most sensitive parameter of liver structure injury, and the serum ALT mainly stems from the damage to the cell membrane [ 20 ]. A previous study displayed that the serum ALT and AST activities were remarkably increased in LPS-treated liver-injury mice, which is in agreement with this study [ 2 ]. Nevertheless, L. paracasei CCFM1223 pretreatment remarkably suppressed the serum AST and ALT activities compared with the LPS group, suggesting that L. paracasei CCFM1223 had a protective effect against ALI.…”

Section: Discussionsupporting

confidence: 93%

“…Lipopolysaccharide (LPS) acts as one of the common endotoxemia that major stem from Gram-negative bacteria. LPS promotes the secretion of inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6) and oxidative stress [malondialdehyde (MDA)] by activating toll-like receptor (Tlr) 4 and suppressing nuclear factor-E2-related factor 2 (Nrf2), and then destroying the intestinal barrier structure and accelerating the development of diseases-related liver [ 2 ]. Therefore, LPS is widely used in the establishment of the ALI model because its symptoms are similar to the clinical symptoms of ALI patients.…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

et al. 2022

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Background: Lactobacillus paracasei CCFM1223, a probiotic previously isolated from the healthy people’s intestine, exerts the beneficial influence of preventing the development of inflammation. Methods: The aim of this research was to explore the beneficial effects of L. paracasei CCFM1223 to prevent lipopolysaccharide (LPS)-induced acute liver injury (ALI) and elaborate on its hepatoprotective mechanisms. Results: L. paracasei CCFM1223 pretreatment remarkably decreased the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in mice with LPS treatment and remarkably recovered LPS-induced the changes in inflammatory cytokines (tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), interleukin (IL)-1β, IL-6, IL-17, IL-10, and LPS) and antioxidative enzymes activities (total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT)). Metagenomic analysis showed that L. paracasei CCFM1223 pretreatment remarkably increased the relative abundance of Catabacter compared with the LPS group but remarkably reduced the relative abundance of [Eubacterium] xylanophilumgroup, ASF356, LachnospiraceaeNK4A136group, and Lachnoclostridium, which is closely associated with the inflammation cytokines and antioxidative enzymes. Furthermore, L. paracasei CCFM1223 pretreatment remarkably increased the colonic, serum, and hepatic IL-22 levels in ALI mice. In addition, L. paracasei CCFM1223 pretreatment remarkably down-regulated the hepatic Tlr4 and Nf-kβ transcriptions and significantly up-regulated the hepatic Tlr9, Tak1, Iκ-Bα, and Nrf2 transcriptions in ALI mice. Conclusions: L. paracasei CCFM1223 has a hepatoprotective function in ameliorating LPS-induced ALI by regulating the “gut–liver” axis.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

et al. 2022

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“…The liver indices in the LPS group was also increased significantly, which might be due to the inflammatory response induced by the LPS. After injection, LPS would activate the nuclear factor kappa-B ( NF-κB ) signaling pathway, produce downstream proinflammatory cytokines and chemokines, and enhance liver tissue inflammation and significant expansion of hepatic sinusoids ( Chen et al, 2021 ; Xu et al, 2021 ). There were no reports similar to the substantial increase in the organ indices of the glandular stomach caused by multiple injections of LPS.…”

Section: Discussionmentioning

confidence: 99%

The influence of immune stress induced by Escherichia coli lipopolysaccharide on the pharmacokinetics of danofloxacin in broilers

et al. 2022

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This study aimed to determine whether the challenge from Escherichia coli ( E. coli ) lipopolysaccharide ( LPS ) affects the pharmacokinetics of danofloxacin in broilers. Twenty 1-day-old Arbor Acres ( AA ) broilers were equally and randomly divided into 2 groups. When the chickens were 23, 25, 27, and 29 days old, E. coli LPS (1 mL; 0.5 mg/kg body weight [ BW ]) and sterile saline (1 mL) were intraperitoneally injected into the two groups. After the last injection, danofloxacin was given to all chickens by gavage at the dose of 5 mg/kg BW. Then serum and plasma samples at each time point were collected through the wing vein. Danofloxacin concentrations in plasma were detected through the high-performance liquid chromatography ( HPLC ) method and subjected to noncompartmental analysis using Phoenix software. The levels of chicken interleukin-1β ( IL-1β ) and corticosterone ( CORT ) in serum were measured by the Enzyme-linked immunosorbent assay ( ELISA ) kit. In addition, after the collection of plasma or serum samples, 7 chickens (31 days of age) in each group were killed to calculate the organ indices. Compared with the control group, the challenge of LPS significantly decreased the parameters of AUC 0-∞ , C max , and t 1/2λz and increased the parameters of T max and λz. Additionally, in the LPS group, the absorption time of danofloxacin was prolonged; however, the elimination was accelerated, which resulted in reduced internal exposure.

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“…This accelerates the accumulation of neutrophils and the release of additional cytokines and chemokines . Acute liver injury can be established in animals using the intraperitoneal administration of LPS, which is a representative model of this disease and has been universally applied to assess the pathogenesis of acute liver disease and the process of pharmacological interventions. − Although some anti-inflammatory chemicals have been developed to treat acute liver injury, clinical data promulgated that some drugs have had some adverse reactions . Therefore, researchers are committed to looking for new effective technologies and strategies to improve acute liver injury and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Microbiome-Derived Inosine on Lipopolysaccharide-Induced Acute Liver Damage and Inflammation in Mice via Mediating the TLR4/NF-κB Pathway

Guo

Xiang

Mao

et al. 2021

J. Agric. Food Chem.

102

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This research assessed the anti-inflammatory and hepatoprotective properties of inosine and the associated mechanism. Inosine pretreatment significantly reduced the secretion of several inflammatory factors and serum alanine transaminase (ALT) and aspartate amino transferase (AST) levels in a dose-dependent manner compared with the lipopolysaccharide (LPS) group. In LPS-treated mice, inosine pretreatment significantly reduced the ALT and malondialdehyde (MDA) concentration and significantly elevated the antioxidant enzyme activity. Furthermore, inosine pretreatment significantly altered the relative abundance of the genera, Bifidobacterium, Lachnospiraceae UCG-006, and Muribaculum. Correlation analysis showed that Bifidobacterium and Lachnospiraceae UCG-006 were positively related to the cecal short-chain fatty acids but negatively related to the serum IL-6 and hepatic AST and ALT levels. Notably, inosine pretreatment significantly modulated the hepatic TLR4, MYD88, NF-κB, iNOS, COX2, AMPK, Nfr2, and IκB-α expression. These results suggested that inosine pretreatment alters the intestinal microbiota structure and improves LPS-induced acute liver damage and inflammation through modulating the TLR4/NF-κB signaling pathway.

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Study on the protective effect and mechanism of Dicliptera chinensis (L.) Juss (Acanthaceae) polysaccharide on immune liver injury induced by LPS

Cited by 16 publications

References 37 publications

Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

The influence of immune stress induced by Escherichia coli lipopolysaccharide on the pharmacokinetics of danofloxacin in broilers

Protective Effects of Microbiome-Derived Inosine on Lipopolysaccharide-Induced Acute Liver Damage and Inflammation in Mice via Mediating the TLR4/NF-κB Pathway

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