Evaluation of the gut microbiota after metformin intervention in children with obesity: A metagenomic study of a randomized controlled trial

Pastor-Villaescusa, Belén; Plaza‐Díaz, Julio; Egea-Zorrilla, Alejandro; Leis, Rosaura; Bueno, Gloria; Hoyos, Ramiro Caballero; Vázquez-Cobela, Rocío; Latorre, Miriam; Cañete, María Dolores; Caballero-Villarraso, Javier; Gil, Ángel; Cañete, Ramón; Aguilera, Concepción M.

doi:10.1016/j.biopha.2020.111117

Cited by 7 publications

(5 citation statements)

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“…Various medications can influence the gut microbiome in other systemic diseases. [ 17 , 18 ] In this study, we investigated whether the model could be used for treated membranous nephropathies (TMNs). The results showed that POD value level was markedly higher in TMNs than that in HCs, with an AUC of 96.44% (cut‐off value, 0.6315; sensitivity, 0.9373; specificity, 0.9122, Figure 3I,J ).…”

Section: Resultsmentioning

confidence: 99%

Gut Microbiome Analysis Can Be Used as a Noninvasive Diagnostic Tool and Plays an Essential Role in the Onset of Membranous Nephropathy

et al. 2022

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Membranous nephropathy (MN) is a common cause of nephrotic syndrome.The aim is to establish a non-invasive diagnostic model of MN using differential gut microbiome analysis, and to explore the relationship between the gut microbiome and MN pathogenesis in vivo. 825 fecal samples from MN patients and healthy participants are collected from multiple medical centers across China. Key operational taxonomic units (OTUs) obtained through 16S rRNA sequencing are used to establish a diagnostic model. A rat model of MN is developed to explore the relationship between the gut microbiome and the pathogenesis of MN. The diversity and richness of the gut microbiome are significantly lower in patients with MN than in healthy individuals. The diagnostic model based on seven OTUs achieves an excellent efficiency of 98.36% in the training group and also achieves high efficiency in cross-regional cohorts. In MN rat model, gut microbiome elimination prevents model establishment, but fecal microbiome transplantation restores the phenotype of protein urine. Gut microbiome analysis can be used as a non-invasive tool for MN diagnosis. The onset of MN depends on the presence of naturally colonized microbiome. Early intervention in the gut microbiome may help reduce urinary protein level in MN.

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Section: Resultsmentioning

confidence: 99%

Gut Microbiome Analysis Can Be Used as a Noninvasive Diagnostic Tool and Plays an Essential Role in the Onset of Membranous Nephropathy

et al. 2022

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“…Diet has a considerable effect on the composition of gastrointestinal microbiota and an unhealthy diet, among other factors, alters its composition and induces gastric microbial dysbiosis [ 52 ]. The gut microbiome is the genetic material of all the microbes that live on and inside the human body [ 53 , 54 , 55 ]. H. pylori colonization results in the alteration of gastric microbiota and a reduction in bacterial diversity [ 56 , 57 ], thus modifying the host’s gut microbiome [ 58 ].…”

Section: The Impact Of Diet Patterns In Helicobacter Pylori Infectionmentioning

confidence: 99%

Impact of Dietary Patterns on H. pylori Infection and the Modulation of Microbiota to Counteract Its Effect. A Narrative Review

et al. 2021

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Helicobacter pylori (H. pylori) is a Gram-negative bacterium that colonizes the stomach and can induce gastric disease and intra-gastric lesions, including chronic gastritis, peptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue lymphoma. This bacterium is responsible for long-term complications of gastric disease. The conjunction of host genetics, immune response, bacterial virulence expression, diet, micronutrient availability, and microbiome structure influence the disease outcomes related to chronic H. pylori infection. In this regard, the consumption of unhealthy and unbalanced diets can induce microbial dysbiosis, which infection with H. pylori may contribute to. However, to date, clinical trials have reported controversial results and current knowledge in this field is inconclusive. Here, we review preclinical studies concerning the changes produced in the microbiota that may be related to H. pylori infection, as well as the involvement of diet. We summarize and discuss the last approaches based on the modulation of the microbiota to improve the negative impact of H. pylori infection and their potential translation from bench to bedside.

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“…Previous studies have shown that drugs, such as corticosteroids, tacrolimus, or metformin, can have varying degrees of impact on the host’s gut microbiota ( Jiang et al, 2018 ; Pastor-Villaescusa et al, 2021 ; Petrullo et al, 2022 ). However, in our project, drug application did not seem to affect the effectiveness of the diagnostic model.…”

Section: Resultsmentioning

confidence: 99%

Characterization and diagnostic value of the gut microbial composition in patients with minimal change disease

et al. 2022

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Background: Minimal change disease (MCD) is one of the most common causes of primary nephrotic syndrome with high morbidity. This study aimed to explore the typical alterations of gut microbiota in MCD and establish a non-invasive classifier using key gut microbiome. We also aimed to evaluate the therapeutic efficiency of gut microbiota intervention in MCD through animal experiments.Methods: A total of 222 stool samples were collected from MCD patients and healthy controls at the First Affiliated Hospital of Zhengzhou University and Shandong Provincial Hospital for 16S rRNA sequencing. Optimum operational taxonomic units (OTUs) were obtained for constructing a diagnostic model. MCD rat models were established using doxorubicin hydrochloride for exploring the therapeutic efficiency of gut microbial intervention through fecal microbiota transplantation (FMT).Results: The α-diversity of gut microbiota decreased in MCD patients when compared with healthy controls. The relative abundance of bacterial species also changed significantly. We constructed a diagnostic model based on eight optimal OTUs and it achieved efficiency of 97.81% in discovery cohort. The high efficiency of diagnostic model was also validated in the patients with different disease states and cross-regional cohorts. The treatment partially recovered the gut microbial dysbiosis in patients with MCD. In animal experiments, likewise, the gut microbiota changed sharply in MCD rats. However, gut microbial interventions did not reduce urinary protein or pathological kidney damage.Conclusion: Gut Microbiota shifts sharply in both patients and rats with MCD. Typical microbial changes can be used as biomarkers for MCD diagnosis. The gut microbiota compositions in patients with MCD tended to normalize after treatment. However, the intervention of gut microbiota seems to have no therapeutic effect on MCD.

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Evaluation of the gut microbiota after metformin intervention in children with obesity: A metagenomic study of a randomized controlled trial

Cited by 7 publications

References 42 publications

Gut Microbiome Analysis Can Be Used as a Noninvasive Diagnostic Tool and Plays an Essential Role in the Onset of Membranous Nephropathy

Gut Microbiome Analysis Can Be Used as a Noninvasive Diagnostic Tool and Plays an Essential Role in the Onset of Membranous Nephropathy

Impact of Dietary Patterns on H. pylori Infection and the Modulation of Microbiota to Counteract Its Effect. A Narrative Review

Characterization and diagnostic value of the gut microbial composition in patients with minimal change disease

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