Three-Dimensional Vessel Segmentation in Whole-Tissue and Whole-Block Imaging Using a Deep Neural Network

Ohnishi, Takashi; Teplov, A. A.; Kawata, Noboru; Ibrahim, Kareem; Ntiamoah, Peter; Fırat, Canan; Haneishi, Hideaki; Hameed, Meera; Shia, Jinru; Yagi, Yukako

doi:10.1016/j.ajpath.2020.12.008

Cited by 10 publications

(6 citation statements)

References 21 publications

(13 reference statements)

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“…Furthermore, since a much larger number of micro-CT images can be produced per specimen compared to conventional pathology images, it can save labor in the evaluation of obtaining images for clinical application. Ongoing development of artificial intelligence technologies has the potential to develop micro-CT image interpretation support systems that directly lead to pathological diagnosis by combining deep machine learning with biomedical image processing algorithms such as texture matching and non-rigid registration algorithms 22 – 24 .…”

Section: Discussionmentioning

confidence: 99%

A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

Sakamoto

Nishimura

Teplov

et al. 2022

Sci Rep

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Endoscopic submucosal dissection can remove large superficial gastrointestinal lesions in en bloc. A detailed pathological evaluation of the resected specimen is required to assess the risk of recurrence after treatment. However, the current method of sectioning specimens to a thickness of a few millimeters does not provide information between the sections that are lost during the preparation. In this study, we have produced three-dimensional images of the entire dissected lesion for nine samples by using micro-CT imaging system. Although it was difficult to diagnose histological type on micro-CT images, it successfully evaluates the extent of the lesion and its surgical margins. Micro-CT images can depict sites that cannot be observed by the conventional pathological diagnostic process, suggesting that it may be useful to use in a complementary manner.

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Section: Discussionmentioning

confidence: 99%

A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

Sakamoto

Nishimura

Teplov

et al. 2022

Sci Rep

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“…FFPE blocks were scanned using a micro-CT scanner (Nikon Metrology NV, Leuven, Belgium) for 8 h to obtain WBI as previously described [ 17 ]. Image slices were reconstructed using modified Feldkamp-filtered back-projection algorithms with CDPro3D (Nikon Metrology).…”

Section: Methodsmentioning

confidence: 99%

“…Micro-focus X-ray computed tomography (micro-CT) provides a nondestructive reconstruction of high-resolution digital three-dimensional (3D) serial images up to 1 mm from the entire formalin-fixed paraffin-embedded (FFPE) tissue blocks [ 17 , 18 ]. Although micro-CT itself was initially developed for industrial and material science, recent studies have shown the efficacy of histological evaluation in breast, lung, and thyroid cancer using whole-block imaging (WBI) [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography

et al. 2022

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Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is unclear. We aimed to clarify the efficacy of WBI in the whole-mount specimens of locally advanced rectal cancer. A total of 237 whole-mount formalin-fixed paraffin-embedded blocks from 13 patients with rectal cancer who underwent surgical treatment were enrolled and scanned with micro-CT to generate three-dimensional images. WBI was evaluated following the conventional pathological review of the corresponding whole-slide imaging (WSI). WBI identified all tumor sites detected using WSI. Furthermore, WBI revealed one additional tumor site, which was not detected using WSI. Tumor resection margin was significantly closer to the soft-tissue edge when measured using WBI (7.7 mm vs. 6.6 mm, p < 0.01). Seventy-six percent of tumor deposits on WSI were changed according to the evidence of tumor interaction with the surrounding tissues confirmed using WBI. Furthermore, WBI revealed 25 additional lymph nodes, six of which were metastatic. The combination of conventional hematoxylin and eosin-stained imaging and WBI may contribute to an accurate pathological assessment.

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“…Although histopathology is a time-honored method and constitutes the gold standard for the examination of human tumors, both conventional glass slides and their relatively new alternative, digital whole slide images (WSIs) obtained by scanning the glass slides, provide only two-dimensional (2D) information on a tumor, which is in fact a threedimensional (3D) entity [2,3]. Although 3D images can be reconstructed from serial WSIs, this is difficult and time-consuming, and it requires the whole paraffin block to be consumed, thereby disrupting the integrity of the tissue examined [4][5][6]. Additionally, in a histopathological examination, the amount of submitted tissue and the size of the tissue cassettes limit the material that is examined.…”

Section: Histopathological Imaging and Its Limitationsmentioning

confidence: 99%

The Value of Micro-CT in the Diagnosis of Lung Carcinoma: A Radio-Histopathological Perspective

Dizbay Sak,

Sevim,

Buyuksungur

et al. 2023

Diagnostics

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Micro-computed tomography (micro-CT) is a relatively new imaging modality and the three-dimensional (3D) images obtained via micro-CT allow researchers to collect both quantitative and qualitative information on various types of samples. Micro-CT could potentially be used to examine human diseases and several studies have been published on this topic in the last decade. In this study, the potential uses of micro-CT in understanding and evaluating lung carcinoma and the relevant studies conducted on lung and other tumors are summarized. Currently, the resolution of benchtop laboratory micro-CT units has not reached the levels that can be obtained with light microscopy, and it is not possible to detect the histopathological features (e.g., tumor type, adenocarcinoma pattern, spread through air spaces) required for lung cancer management. However, its ability to provide 3D images in any plane of section, without disturbing the integrity of the specimen, suggests that it can be used as an auxiliary technique, especially in surgical margin examination, the evaluation of tumor invasion in the entire specimen, and calculation of primary and metastatic tumor volume. Along with future developments in micro-CT technology, it can be expected that the image resolution will gradually improve, the examination time will decrease, and the relevant software will be more user friendly. As a result of these developments, micro-CT may enter pathology laboratories as an auxiliary method in the pathological evaluation of lung tumors. However, the safety, performance, and cost effectiveness of micro-CT in the areas of possible clinical application should be investigated. If micro-CT passes all these tests, it may lead to the convergence of radiology and pathology applications performed independently in separate units today, and the birth of a new type of diagnostician who has equal knowledge of the histological and radiological features of tumors.

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Three-Dimensional Vessel Segmentation in Whole-Tissue and Whole-Block Imaging Using a Deep Neural Network

Cited by 10 publications

References 21 publications

A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography

The Value of Micro-CT in the Diagnosis of Lung Carcinoma: A Radio-Histopathological Perspective

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