A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

Sakamoto, Hirotsugu; Nishimura, Makoto; Teplov, A. A.; Leung, Galen; Ntiamoah, Peter; Cesmecioglu, Emine; Kawata, Noboru; Ohnishi, Takashi; Ibrahim, Kareem; Shia, Jinru; Yagi, Yukako

doi:10.1038/s41598-022-13907-6

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…Unfortunately, this method does not allow one to avoid the disadvantages of classical histological examination in the form of unavoidable damage to the sample during the section [ 118 ]. Recent studies have shown the efficacy of histological evaluation in breast, lung, colon, gastric, and thyroid cancer using whole-block imaging (WBI) via micro-CT [ 119 , 120 , 121 , 122 , 123 ]. The role of whole-tissue imaging can be increased due to the necessity for accurate evaluation of margins of surgical resection, initial treatment response, and response to neoadjuvant chemotherapy, and would be considered as a helping hand for pathologists.…”

Section: Untrodden Path: Vasculature Access In Sarcomasmentioning

confidence: 99%

Imaging Hallmarks of Sarcoma Progression Via X-ray Computed Tomography: Beholding the Flower of Evil

Popova

Tkachev

Reshetov

et al. 2022

Cancers

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Sarcomas are a leading cause of cancer death in individuals younger than 20 years of age and represent the largest group of rare solid tumors. To date, more than 100 morphological subtypes of sarcomas have been described, among which epidemiology, clinical features, management, and prognosis differ significantly. Delays and errors in the diagnosis of sarcomas limit the number of effective therapeutic modalities and catastrophically worsen the prognosis. Therefore, the development of an algorithm for the early accurate diagnosis of sarcomas seems to be as important as the development of novel therapeutic advances. This literature review aims to summarize the results of recent investigations regarding the imaging of sarcoma progression based on the use of X-ray computed tomography (CT) in preclinical studies and in current clinical practice through the lens of cancer hallmarks. We attempted to summarize the main CT imaging features of soft-tissue and bone sarcomas. We noted the development of new molecular markers with high specificity to antibodies and chemokines, which are expressed in particular sarcoma subtypes to reach tumor type-specific imaging. We demonstrate the possibility of the use of X-ray computed microtomography (micro-CT) for non-destructive 3D visualization of solid tumors by increasing the visibility of soft tissues with X-ray scattering agents. Based on the results of recent studies, we hypothesize that micro-CT enables the visualization of neovascularization and stroma formation in sarcomas at high-resolution in vivo and ex vivo, including the novel techniques of whole-block and whole-tissue imaging. Finding correlations between CT, PET/CT, and micro-CT imaging features, the results of the histopathological specimen examination and clinical outcomes may significantly increase the accuracy of soft-tissue and bone tumor diagnostics, which leads to the initiation of appropriate histotype-specific management in a timely manner and, consequently, to improved outcomes.

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Section: Untrodden Path: Vasculature Access In Sarcomasmentioning

confidence: 99%

Imaging Hallmarks of Sarcoma Progression Via X-ray Computed Tomography: Beholding the Flower of Evil

Popova

Tkachev

Reshetov

et al. 2022

Cancers

View full text Add to dashboard Cite

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Analysis of 3D pathology samples using weakly supervised AI

Song,

Williams,

Williamson

et al. 2024

Cell

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X-ray phase-contrast 3D virtual histology characterises complex tissue architecture in colorectal cancer

Svetlove,

Griebel,

Albers

et al. 2023

Front. Gastroenterol.

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Precise morphological analysis of tumour tissue samples is crucial for accurate diagnosis and staging of colorectal cancer (CRC), but remains limited by the 2D nature of conventional histology. Our aim is to offer a 3D representation of tissue samples by means of X-ray-based imaging to facilitate the evaluation of clinically relevant features in cancer tissue, a process that is currently subject to various restrictions. In this study, we show that propagation-based synchrotron radiation-based free propagation phase-contrast microcomputed tomography (SRµCT) is suitable for the generation of 3D tumour volumes with 2-µm voxel size using standard formalin-fixed, paraffin-embedded tissue from CRC patients and provides sufficient contrast for virtual histology. We demonstrate that, using an existing registration pipeline, a 2D histologic haematoxylin–eosin slice can be placed in the context of the 3D µCT volume. The precisely registered histologic section can then be used as a “seed point” for the segmentation and depiction of major histologic features. This approach allows for a more comprehensive understanding of the organisation of the tumour in space with respect to other structures such as vessels, fat, and lymph nodes, and has the potential to improve patients’ prognostic outcomes.

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A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens

Cited by 4 publications

References 24 publications

Imaging Hallmarks of Sarcoma Progression Via X-ray Computed Tomography: Beholding the Flower of Evil

Imaging Hallmarks of Sarcoma Progression Via X-ray Computed Tomography: Beholding the Flower of Evil

Analysis of 3D pathology samples using weakly supervised AI

X-ray phase-contrast 3D virtual histology characterises complex tissue architecture in colorectal cancer

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