Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia

Dedkov, Vladimir G.; Dolgova, Anna; Safonova, Marina V.; Neverov, Alexey D.; Belova, Oxana A.; Kholodilov, Ivan S.; Matsvay, Alina D.; Speranskaya, A.S.; Khafizov, K.; Karganova, Galina G.

doi:10.1016/j.ttbdis.2020.101612

Cited by 4 publications

(4 citation statements)

References 63 publications

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“…In the last decade, meta-transcriptomic studies have revealed the extensive diversity of the RNA viruses of invertebrates [1,2]. Some of the new viruses are considered to be pathogenic arboviruses and are being extensively studied [46][47][48][49][50]. While the viromes of some blood-sucking ectoparasites, such as mosquitoes and ticks, are relatively wellstudied [6,7,9,10], there are few data on less-known species, such as louse flies [2].…”

Section: Discussionmentioning

confidence: 99%

Section: Multiplication Of Viruses In Mammalian Cellsmentioning

confidence: 99%

“…We tested the ability of the discovered viruses to infect mammalian cells. We used a PEK cell as a model culture because it was previously shown to be able to support the reproduction of different arboviruses, including orbiviruses [46]. BBMRV was detected in ked suspensions #13578 and 13579, and after the first passage of suspensions #13578 and 13580.…”

Section: Multiplication Of Viruses In Mammalian Cellsmentioning

confidence: 99%

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Possible Arbovirus Found in Virome of Melophagus ovinus

Litov¹,

Belova²,

Kholodilov³

et al. 2021

Viruses

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Members of the Lipopteninae subfamily are blood-sucking ectoparasites of mammals. The sheep ked (Melophagus ovinus) is a widely distributed ectoparasite of sheep. It can be found in most sheep-rearing areas and can cause skin irritation, restlessness, anemia, weight loss and skin injuries. Various bacteria and some viruses have been detected in M. ovinus; however, the virome of this ked has never been studied using modern approaches. Here, we study the virome of M. ovinus collected in the Republic of Tuva, Russia. In our research, we were able to assemble full genomes for five novel viruses, related to the Rhabdoviridae (Sigmavirus), Iflaviridae, Reoviridae and Solemoviridae families. Four viruses were found in all five of the studied pools, while one virus was found in two pools. Phylogenetically, all of the novel viruses clustered together with various recently described arthropod viruses. All the discovered viruses were tested on their ability to replicate in the mammalian porcine embryo kidney (PEK) cell line. Aksy-Durug Melophagus sigmavirus RNA was detected in the PEK cell line cultural supernate after the first, second and third passages. Such data imply that this virus might be able to replicate in mammalian cells, and thus, can be considered as a possible arbovirus.

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Section: Discussionmentioning

confidence: 99%

Section: Multiplication Of Viruses In Mammalian Cellsmentioning

confidence: 99%

Section: Multiplication Of Viruses In Mammalian Cellsmentioning

confidence: 99%

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Possible Arbovirus Found in Virome of Melophagus ovinus

Litov¹,

Belova²,

Kholodilov³

et al. 2021

Viruses

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“…Phylogenetic analysis showed that GTOV was genetically related to the known reoviruses ( Supplementary Figure S4 ), in which VP1, VP4 and NS3 were closely related to the Wad Medani virus SUD1952/01 identified in Rhipicephalus in Sudan ( Belaganahalli et al, 2015 ). VP3, VP5, VP6 and NS1 were closely related to the strain Tuva 2012 in Russia ( Dedkov et al, 2021 ), and VP2 was closely related to the strain LEIV-8066Tur in Tajikistan, while NS2 was closely related to the isolate G-673 in India ( Yadav et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Virome of Rhipicephalus ticks by metagenomic analysis in Guangdong, southern China

Guo

Lin³

et al. 2022

Front. Microbiol.

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Tick-borne viruses (TBVs) have increasingly caused a global public health concern. This study collected Rhipicephalus ticks in Guangdong, southern China to identify RNA viruses. Meta-transcriptome analysis revealed the virome in Rhipicephalus ticks, resulting in the discovery of 10 viruses, including Lihan tick virus, Brown dog tick phlebovirus 1 and 2 in the family Phenuiviridae, Mivirus and Wuhan tick virus 2 in the family Chuviridae, Wuhan tick virus 1 in the family Rhabdoviridae, bovine hepacivirus in the family Flaviviridae, Guangdong tick quaranjavirus (GTQV) in the family Orthomyxoviridae, Guangdong tick orbivirus (GTOV) in the family Reoviridae, and Guangdong tick Manly virus (GTMV) of an unclassified family. Phylogenetic analysis showed that most of these TBVs were genetically related to the strains in countries outside China, and GTQV, GTOV, and GTMV may represent novel viral species. These findings provided evidence of the long-distance spread of these TBVs in Guangdong, southern China, suggesting the necessity and importance of TBV surveillance.

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Arthropod-borne and arthropod-related viruses in Iran and neighboring countries

Azari-hamidian,

Harbach

2023

Parazitologiâ

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Arthropods are very significant for human and veterinary medicine and health because of the burden of diseases caused by the pathogens they transmit. Databases, including the Web of Science, PubMed, Scopus, Google Scholar, CABI, Scientific Information Database, IranMedex and Magiran were searched to the end of December 2022 for publications concerning infections in Iran caused by arboviruses. Pertinent information was extracted and analyzed. Thirty-three viral infections occur in Iran, which are biologically or mechanically known or assumed to be transmitted by arthropods. Information about agents (viruses), distribution (in 31 Iranian provinces), hosts (human and animals) and known vectors in Iran was obtained for each disease. Also, a list of arboviruses was provided for the countries neighboring Iran, including Afghanistan, Armenia, Azerbaijan, Bahrain, Iraq, Kuwait, Oman, Pakistan, Qatar, Saudi Arabia, Turkey, Turkmenistan and the United Arab Emirates, as well as Djibouti, Somalia, Sudan, Syria and Yemen, which do not neighbor Iran but, like Iran, occur in the World Health Organization Eastern Mediterranean Region. This list includes 40 viruses which are not formally recorded in Iran. The viruses are members of 19 genera representing 14 families in which three, four, 20 and 29 viruses are sandfly-borne, biting midge-borne, mosquito-borne and tick-borne, respectively.

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Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia

Cited by 4 publications

References 63 publications

Possible Arbovirus Found in Virome of Melophagus ovinus

Possible Arbovirus Found in Virome of Melophagus ovinus

Virome of Rhipicephalus ticks by metagenomic analysis in Guangdong, southern China

Arthropod-borne and arthropod-related viruses in Iran and neighboring countries

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