Rapid Serotyping of <i>Salmonella</i> Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a <i>Salmonella-</i>Specific Gene

Yamasaki, Eiki; Matsuzawa, Shigeru; Takeuchi, Kaoru; Morimoto, Yoko; Ikeda, Tetsuya; Okumura, Keiji; Kurazono, Hisao

doi:10.1089/fpd.2020.2823

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…I137_14430 and stn genes of S. Pullorum were aligned with the genome of C. freundii, and no significant similarity was found in C. freundii genome for both I137_14430 sequence (Supplementary Figure S3B) and stn sequence (Supplementary Figure S3C). Furthermore, the gene stn is extensively employed as the reference control of the Salmonella genus (26)(27)(28). Thus, based on the sequence characteristics, three sets of primers of torT, I137_14430, and stn were designed.…”

Section: Implementation Of Multiplex Pcr Test On Chicken Egg Samplesmentioning

confidence: 99%

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

Tan

Xiong

et al. 2023

Front. Vet. Sci.

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Most cases of chicken salmonellosis are caused by Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum, which lead to a significant morbidity and fatality rate. Although the conventional Kaufmann-White scheme is the reliable method for the serotyping of Salmonella, it does not distinguish between closely related biotypes like S. Pullorum and S. Gallinarum. Herein, we conducted a single one-step multiplex PCR assay that can identify and distinguish between S. Pullorum and S. Gallinarum in an accurate manner. This PCR method was based on three genes, including torT for S. Pullorum identification, I137_14430 for S. Gallinarum identification, and stn as the genus-level reference gene for Salmonella. By comparing S. Pullorum to S. Gallinarum and other serovars of Salmonella, in silico study revealed that only the former has a deletion of 126 bp-region in the carboxyl terminus of torT. The I137_14430 gene does not exist in S. Gallinarum. However, it is present in all other Salmonella serotypes. The multiplex PCR approach utilizes unique sets of primers that are intended to specifically target these three different genes. The established PCR method was capable of distinguishing between the biovars Pullorum and Gallinarum from the 29 distinct Salmonella serotypes as well as the 50 distinct pathogens that are not Salmonella, showing excellent specificity and exclusivity. The minimal amount of bacterial cells required for PCR detection was 100 CFU, while the lowest level of genomic DNA required was 27.5 pg/μL for both S. Pullorum and S. Gallinarum. After being implemented on the clinical Salmonella isolates collected from a poultry farm, the PCR test was capable of distinguishing the two biovars Pullorum and Gallinarum from the other Salmonella strains. The findings of the PCR assay were in line with those of the traditional serotyping and biochemical identification methods. This new multiplex PCR could be used as a novel tool to reinforce the clinical diagnosis and differentiation of S. Pullorum and S. Gallinarum, particularly in high-throughput screening situations, providing the opportunity for early screening of infections and, as a result, more effective management of the illness among flocks.

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Section: Implementation Of Multiplex Pcr Test On Chicken Egg Samplesmentioning

confidence: 99%

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

Tan

Xiong

et al. 2023

Front. Vet. Sci.

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“…Sus mecanismos de acción se presentan de manera general mediante la presencia de fiebre dado a que estas estimulan la liberación de pirógenos endógenos, que son proteínas, que actúan sobre el centro termorregulador del cerebro (Yang et al, 2018). Enterotoxina La enterotoxina de Salmonella (stn) es codificada por un gen observado en especies de Salmonella pero su función en la patogenia no ha sido dilucidada en detalle (Yamasaki et al, 2021). Estas son exotoxinas que influyen en el intestino delgado, debido a que causan una segregación de líquidos de manera masiva en la luz intestinal.…”

Section: Antígeno Vibunclassified

Salmonella spp como contaminante de la carne de pollo: una revisión

et al. 2023

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El objetivo de la investigación fue profundizar en los riesgos que causa la presencia de Salmonella spp, en la carne de pollo contaminada, características de su genoma, el tipo de infección que provoca, los síntomas clínicos, vías de transmisión, entre otros factores de riesgo, tanto en los animales como en el hombre. La Salmonella por su actividad en un pH óptimo de 6,7-6,8 junto a su capacidad de resistencia a sales biliares, capacidad patógena; fimbrias para su adhesión a las células del hospedero, plásmidos, islas de patogenicidad, antígenos polisacáridos O, Vi, entre otros, permiten y favorecen la replicación de la bacteria en el organismo y en alimentos, siendo un grave problema de salud pública por ser una de las principales causas de morbilidad y mortalidad en las personas en todo el mundo.

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Nontyphoid Salmonella in Farm Animals and Food Products in the Middle East and North Africa: A Systematic Review

Bellil,

Mairi,

Kendi

et al. 2023

Future Microbiol.

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Aim: This study aimed to document the prevalence, serotype distribution and antibiotic resistance of nontyphoidal Salmonella in animal food products from Middle East/North Africa (MENA) countries. Methods: Peer-reviewed articles published from 1 January 2011 to 7 March 2023 were included and the data were narratively synthesized and statistically analyzed to estimate and compare the overall prevalence. Results: The authors found a high prevalence of Salmonella in MENA countries (12.80%), with the highest prevalence in Lebanon (41.10%). Poultry had a higher prevalence of Salmonella (14.49%) than livestock (9.62%). Salmonella enteritidis was the most commonly identified serotype (21.99%), and sulfamethoxazole had the highest resistance rate (78.81%). Conclusion: The authors emphasize the importance of implementing control measures in MENA countries to limit the spread of the Salmonella pathogen.

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Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella-Specific Gene

Cited by 3 publications

References 21 publications

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

Salmonella spp como contaminante de la carne de pollo: una revisión

Nontyphoid Salmonella in Farm Animals and Food Products in the Middle East and North Africa: A Systematic Review

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