32P-postlabelling analysis of DNA adducts and EROD induction as biomarkers of genotoxin exposure in dab (Limanda limanda) from British coastal waters

Lyons, Brett P.; Stewart, C. P.; Kirby, Mark F.

doi:10.1016/s0141-1136(00)00111-2

Cited by 9 publications

(7 citation statements)

References 7 publications

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“…The analysis of hepatic DNA adducts failed to distinguish diVerences between the two sites (Table 1) and this conWrms previous studies, which suggest that at the majority of oVshore locations DNA adducts are usually within the limits considered to represent a background level (Lyons et al, 2000;CEFAS, 2003). However, DNA strand breaks were shown to be signiWcantly elevated (p < 0.05) in the erythrocytes of dab collected from Red Wharf Bay over with those from South Cardigan Bay.…”

Section: Introductionsupporting

confidence: 87%

“…Sections of liver tissue were removed, placed into individual histological cassettes, transferred to 10% neutral buVered formalin and processed for histopathology as described previously (Stentiford et al, 2003). Liver and blood samples were also collected for the analysis of DNA adducts and DNA strand breaks, respectively (Belpaeme et al, 1998;Lyons et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Feist et al (2004). A subset of 5 males were sampled for DNA adduct analysis using the 32 P-postlabelling assay (adducted nucleotides per 10 8 undamaged nucleotides) as described previously (Lyons et al, 2000). Ten Wsh (5 males and 5 females) were analysed using the alkaline version of the comet assay (tail moment) adapted from the method described by Belpaeme et al (1998).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A biological effects monitoring survey of Cardigan Bay using flatfish histopathology, cellular biomarkers and sediment bioassays: Findings of the Prince Madog Prize 2003

Lyons

Stentiford

Bignell

et al. 2006

Marine Environmental Research

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Section: Introductionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A biological effects monitoring survey of Cardigan Bay using flatfish histopathology, cellular biomarkers and sediment bioassays: Findings of the Prince Madog Prize 2003

Lyons

Stentiford

Bignell

et al. 2006

Marine Environmental Research

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“…Many studies correlate environmental levels of pollutants to actual biological effects on organisms by measuring a variety of different endpoints or biomarkers (Varanasi et al 1989; Bickham 1990; Varanasi and Stein 1991; Lyons et al 2000; Siu et al 2004; Machella et al 2005; Matson et al 2005). These studies show the importance of choosing appropriate endpoints when measuring the effects of chemical mixtures that can potentially have several mechanisms of action, such as PAH mixtures.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity in Atlantic killifish (Fundulus heteroclitus) from a PAH-contaminated Superfund site on the Elizabeth River, Virginia

et al. 2011

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The Atlantic Wood Industries Superfund site (AWI) on the Elizabeth River in Portsmouth, VA is heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) from a wood treatment facility. Atlantic killifish, or mummichog (Fundulus heteroclitus), at this Superfund site are exposed to very high concentrations of several carcinogens. In this study, we measured PAH concentrations in both fish tissues and sediments. Concurrently, we assessed different aspects of genotoxicity in the killifish exposed in situ. Both sediment and tissue PAH levels were significantly higher in AWI samples, relative to a reference site, but the chemistry profile was different between sediments and tissues. Killifish at AWI exhibited higher levels of DNA damage compared to reference fish, as measured via the flow cytometric method (FCM), and the damage was consistent with sediment PAH concentrations. Covalent binding of benzo[a]pyrene (BaP) metabolites to DNA, as measured via LC-MS/MS adduct detection methods, were also elevated and could be partially responsible for the DNA damage. Using similar LC-MS/MS methods, we found no evidence that oxidative DNA adducts had a role in observed genotoxicity.

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“…DNA adducts are thus probably one of the most relevant indicators of genetic damage due to exposure to toxins. The detection of DNA adducts is widely used as biomarker of aquatic contamination (Dunn et al 1987;Kurelec et al 1989a;Varanasi et al 1989;Ray et al 1991;Stein et al 1994;French et al 1996;El Adlouni et al 1995;Harvey et al 1997;Pfau et al 1997;Burgeot et al 1996;Boillot et al 1997;Ericson et al 1998;Ericson and Lasson 2000;Lyons et al 1999Lyons et al , 2000Stephensen et al 2000). Recently, Rice et al (2000) used English Sole (Pleuronectes vetulus) to test the genotoxic impact of polluted sediment.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Impact of ‘Erika’ Petroleum Fuel on Liver of the Fish Solea Solea

Amat¹,

Burgeot²,

Castegnaro³

et al.

Environmental Chemistry

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On 12 December 1999, one third of the load of the tanker 'Erika' , amounting to about 10 000 t crude oil flowed into sea waters close to the French Atlantic Coast (Finistère region). The spilled oil was fuel No. 6, a heavy or residual fuel that is a complex mixture of polycyclic aromatic compounds (PAC) of high molecular weight. Some polycyclic aromatic compounds are genotzoxic substances that induce carcinogenic lesions in laboratory animals. DNA adducts, reflecting genotoxic effects, are used as biomarker of early pollution. In this study, the genotoxic impact of the 'Erika' oil was assessed by studying the presence of DNA adduct in the liver of immature fishes (Solea solea) from four locations of the French Brittany coasts, two, six and nine months after the disaster. Two months after the spill, a high amount of DNA adducts was found in samples from all locations (92 to 290 DNA adduct/10 9 nucleotides). DNA adducts were more persistent in the North than in the South of the affected French Brittany coasts. In September, no significant difference was observed between the locations. When incubated in presence of an 'Erika' fuel extract, DNA adduct patterns similar to those obtained from the liver of Solea solea liver are observed both, in cell culture (HePG2) and, in presence of fish liver microsomes.

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32P-postlabelling analysis of DNA adducts and EROD induction as biomarkers of genotoxin exposure in dab (Limanda limanda) from British coastal waters

Cited by 9 publications

References 7 publications

A biological effects monitoring survey of Cardigan Bay using flatfish histopathology, cellular biomarkers and sediment bioassays: Findings of the Prince Madog Prize 2003

A biological effects monitoring survey of Cardigan Bay using flatfish histopathology, cellular biomarkers and sediment bioassays: Findings of the Prince Madog Prize 2003

Genotoxicity in Atlantic killifish (Fundulus heteroclitus) from a PAH-contaminated Superfund site on the Elizabeth River, Virginia

Genotoxic Impact of ‘Erika’ Petroleum Fuel on Liver of the Fish Solea Solea

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