Development of Model Systems for Plant Rhabdovirus Research

Jackson, Andrew O.; Dietzgen, Ralf G.; Goodin, Michael M.; Li, Zhenghe

doi:10.1016/bs.aivir.2018.06.008

Cited by 16 publications

(12 citation statements)

References 125 publications

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“…Electron microscopic examination of BaCV-infected bean leaves revealed bacilliform particles typical of rhabdoviruses in parenchymal cells. Longitudinal and cross-sectioned particles were located in the cell cytoplasm, commonly at the periphery of an electron-lucent mass of coiled filamentous material, believed to be the viroplasm, where the virus replicates ( Figure 9 a–d) [ 5 , 68 ]. The BaCV bacilliform particles seemed rather scarce in the observed bean tissues.…”

Section: Resultsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown. This information is crucial for the understanding of virus–plant interactions, control strategies, and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plants and are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers, and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papaya cytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil, (ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here, we examine the potential of the Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) to transmit BaCV, its morphological and cytopathological characteristics, and assess the incidence of BaCV across bean producing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimental conditions, by B. tabaci MEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean. Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viral particles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests, identification of the induced symptoms, and the transmission assay. BaCV was detected in five out of the seven states in Brazil included in our study, suggesting that it is widely distributed throughout bean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus.

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Section: Resultsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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“…Reported to be nonpersistently transmitted by Myzus persicae (Valkonen et al, 1992) Nepovirus PBRSV Yes Reported in potato (Jones, 1982) Cannot be excluded (Black, 1970), but seed transmission is reported for maize mosaic virus, another nucleorhabdovirus (Mink, 1993) Yes Reported to be persistently transmitted by Aceratagallia sanguinolenta and Agallia constricta (Black, 1934(Black, , 1943Jackson et al, 2018) Pomovirus…”

Section: Yesmentioning

confidence: 99%

“…No other natural host of economic importance are reported (see Table 10); therefore, no impact is expected on other hosts in the EU territory Nucleorhabdovirus PYDV Yes Not evaluated Yes Growth reduction, leaf distortion and chlorosis and tuber necrosis have been reported in potato (reviewed in Jackson et al, 2018). Therefore, impact is expected on potato, although its magnitude is unclear.…”

Section: Potato Other Hostsmentioning

confidence: 99%

Pest categorisation of non‐EU viruses and viroids of potato

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

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Following a request from the EU Commission, the Panel on Plant Health has addressed the pest categorisation of those viruses and viroids (hereafter referred to as viruses) of Solanum tuberosum and other tuber-forming Solanum spp. (hereafter referred to as potato) which are considered to be either non-EU or of undetermined standing based on a previous EFSA opinion. These viruses belong to different families and genera and either have an established identity or produce consistent symptoms. Plants for planting is the main pathway for entry for all categorised viruses as they can all be transmitted by vegetative propagation. Several categorised viruses have a relatively wide host range and/or are vector-transmitted, increasing the potential for entry. The information currently available on geographical distribution, biology, epidemiology, impact and potential entry pathways has been evaluated with regard to the criteria to qualify as potential Union quarantine pest or as Union regulated non-quarantine pest (RNQP). Since this opinion addresses specifically the non-EU potato viruses, in general these viruses do not meet the criteria assessed by EFSA to qualify as potential Union regulated non-quarantine pests. The following viruses meet the criteria to qualify as potential Union quarantine pest: APLV, APMMV, APMoV, ChiLCV, CYSDV, PAMV, PBRSV, PVH, PVP, PVT, PYDV, PYMV, PYV, PYVV, RCVMV, SALCV, SB26/29, ToCV, ToLCNDV, ToMHaV, ToMoTV, ToSRV and ToYVSV. With the exception of the criterion regarding the potential for consequences in the EU territory, for which the Panel is unable to conclude because of lack of information, AVB, CPSbV, PaLCrV, PapMV, PVB, PVU, SB41 and TVBMV meet all the other criteria to qualify as potential Union quarantine pest. PotLV and WPMV do not qualify as potential Union quarantine pest, since they are not reported to have any impact. For most of the categorised viruses, the conclusions of the Panel have inherent uncertainties, due to the lack of quantitative data on their impact and/or absence or limited availability of information on the biology, epidemiology and geographical distribution.

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“…Plant rhabdovirus gRNA encodes the rhabdoviruses canonical genes: Nucleoprotein (N), Phosphoprotein (P), Matrix (M), Glycoprotein (G), and Large Polymerase (L) (Jackson et al, 2018), in addition to accessory genes (Walker et al, 2011) such as those coding for a cell-to-cell MP such as the 4b gene in lettuce necrotic yellow virus (LNYV) (Dietzgen et al, 2007), gene 3 of rice yellow stunt rhabdovirus (RYSV) (Huang et al, 2005). Furthermore, the gRNA is flanked by UTRs termed 3′ leader and 5′ trailer (Fu, 2005) (Figure 5B).…”

Section: Plant Viruses As Biological Particles the Case Of Inherentlmentioning

confidence: 99%

Plant Viruses in Plant Molecular Pharming: Toward the Use of Enveloped Viruses

2019

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Plant molecular pharming has emerged as a reliable platform for recombinant protein expression providing a safe and low-cost alternative to bacterial and mammalian cells-based systems. Simultaneously, plant viruses have evolved from pathogens to molecular tools for recombinant protein expression, chimaeric viral vaccine production, and lately, as nanoagents for drug delivery. This review summarizes the genesis of viral vectors and agroinfection, the development of non-enveloped viruses for various biotechnological applications, and the on-going research on enveloped plant viruses.

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Development of Model Systems for Plant Rhabdovirus Research

Cited by 16 publications

References 125 publications

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pest categorisation of non‐EU viruses and viroids of potato

Plant Viruses in Plant Molecular Pharming: Toward the Use of Enveloped Viruses

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