Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy

Mangueira, Melissa de Almeida Melo Maciel; Mangueira, Nilton Maciel; Filho, Ozimo Pereira Gama; Oliveira, Márcio Moysés de; Heluy, Renato Albuquerque; Silveira, Landulfo; Dáquer, Egas Caparelli Moniz de Aragão

doi:10.1007/s10103-018-2627-1

Cited by 5 publications

(10 citation statements)

References 44 publications

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“…The present study showed a significant decrease in SFI value and an increase in values of the electrophysiological and morphological measures in the MLS group compared to the corresponding values in the control group. This result was consistent with those of previous studies that found that laser to be effective in accelerating the regeneration of crushed sciatic nerves in Wistar rats 4,5,11,16,[20][21][22][23][24][25][26][27][28][29] . Laser therapy increases adenosine triphosphate production, nerve metabolism, and myelin production, and enhances axonal sprouting, thereby increasing the proliferation of the nerves after crushing 1,7) .…”

Section: Discussionsupporting

confidence: 93%

“…The hair on the left thigh was removed, and it cleaned with an iodine-alcohol solution. The left sciatic nerve was then exposed, detached, and crushed for 30 s at the greater sciatic foramen using non-toothed forceps 4) . The nerve was then re-attached, and the incision was closed with nylon sutures (Ethicon).…”

Section: Methodsmentioning

confidence: 99%

“…Despite significant efforts during rehabilitation, these injuries result in residual loss of sensory and motor functions and are often associated with incomplete recovery 3) . Previous studies have used experimental protocols to induce crushing of the sciatic nerve in rats 4,5) or rabbits to investigate the efficacy of physical modalities, such as photobiomodulation, on the treatment of these injuries 6) .…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

et al. 2021

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To investigate the efficacy of the multi-wave locked system laser therapy on the regeneration of peripheral nerve injuries by evaluating the functional, electrophysiological, and morphological changes of the crushed sciatic nerve in Wistar rats. [Materials and Methods] Sixty male Wistar rats (200-250 g) were randomly assigned to control negative, control positive, or laser groups and subjected to no laser therapy or crushing, to crushing without laser therapy, or crushing followed by multi-wave locked system laser therapy five times/week for four weeks (power=1 W, energy density=10 J/cm 2 , total energy=100 J), respectively. Functional, electrophysiological, and morphometric analyses were performed before and 7, 15, 21, and 28 days after crushing. The sciatic functional index, compound motor action potential amplitude, motor nerve conduction velocity, and nerve and myelin sheath diameters were measured.[Results] The sciatic functional index value decreased significantly, while the compound motor action potential amplitude, motor nerve conduction velocity, nerve diameter, and myelin sheath diameter increased significantly in the laser group post-treatment compared to the values in the control groups. [Conclusion] Multi-wave locked system laser therapy was effective in accelerating the regeneration of crushed sciatic nerves in Wistar rats.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

et al. 2021

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“…Table 4 shows the models of lesions of the sciatic nerve injury used by the authors in their studies; they are as follows: a deadweight machine, 2,5,13,19,21,22 calibrated adjustable tweezer, 12,24 homeostatic tweezer, 1,3,14,23,25,26 and non-serrated homeostatic tweezer. 8,7,20,27 The literary review showed consistent and significant results in favor of photobiomodulation about sciatic nerve regeneration after crush injury, as shown in Table 5.…”

Section: Resultsmentioning

confidence: 81%

“…After the exclusion of articles published as systematic reviews, duplicated titles, inadequate titles, and abstracts, 30 articles were selected for a complete review, and only 19 articles fulfilled all the inclusion criteria. Among these articles, we highlight that the most used wavelengths were 660 nm 1,2,7,[18][19][20] and 830 nm, 2,12,21,22 the powers were 30 2,18,19,21,22 and 40 mW 5,7 and the energy densities were 4 1,3,5,23 and 10 2,5,7,13,16,19,21 J/cm 2 . Tables 1-3 describe the experimental design used in the studies selected for this review and the parameters of the LLLT and LED.…”

Section: Resultsmentioning

confidence: 99%

Photobiomodulation in Sciatic Nerve Crush Injuries in Rodents: A Systematic Review of the Literature and Perspectives for Clinical Treatment

et al. 2020

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Objective: The aim of the study was to perform a literature review to analyze the effect of photobiomodulation in experimental studies on peripheral nerve regeneration after sciatic nerve crush injury in rodents. Methods: A bibliographic search was performed in the electronic databases, including MEDLINE (PubMed), SCOPUS, and SciELO, from 2008 to 2018. Results: A total of 1912 articles were identified in the search, and only 19 fulfilled all the inclusion criteria. Along with the parameters most found in the manuscripts, the most used wavelengths were 660 nm and 830 nm, power of 30 and 40 mW, and energy density of 4 and 10 J/cm2 . For total energy throughout the intervention period, the lowest energy found with positive effects was 0.70 J, and the highest 1.141 J. Seventeen studies reported positive effects on nerve regeneration. The variables selected to analyze the effect were: Sciatic Functional Index (SFI), Static Sciatic Index (SSI), morphometric, morphological, histological, zymographic, electrophysiological, resistance mechanics and range of motion (ROM). The variety of parameters used in the studies demonstrated that there is yet no pre-determined protocol for treating peripheral nerve regeneration. Only two studies by different authors used the same power, energy density, beam area, and power density. Conclusion: It was concluded that the therapeutic window of the photobiomodulation presents a high variability of parameters with the wavelength (632.8 to 940 nm), power (5 to 170 mW) and energy density (3 to 280 J /cm2 ), promoting nerve regeneration through the expression of cytokines and growth factors that aid in modulating the inflammatory process, improving morphological aspects, restoring the functionality to the animals in a brief period.

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Dose-dependent effect of the pulsed Nd:YAG laser in the treatment of crushed sciatic nerve in Wister rats: an experimental model

et al. 2020

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Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy

Cited by 5 publications

References 44 publications

Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

Photobiomodulation in Sciatic Nerve Crush Injuries in Rodents: A Systematic Review of the Literature and Perspectives for Clinical Treatment

Dose-dependent effect of the pulsed Nd:YAG laser in the treatment of crushed sciatic nerve in Wister rats: an experimental model

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