Increased immunoreactivity for TRPM8 in cutaneous squamous cell carcinoma

Oh, Shin-Taek; Yang, Keum Jin; Kim, Y. H.; Bae, Jong Myon; Park, Hue Jung; Kim, J. W.; Park, Y. M.

doi:10.1111/cup.13358

Cited by 4 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After coding the slides of all cases, they were examined and scored. Positive expression was given when apparent membranous and/or cytoplasmic and/or nuclear staining was found (11) . Semiquantitative scoring system was applied.…”

Section: Interpretation Of Immunostaining Resultsmentioning

confidence: 99%

Expression of Homeobox A9 (HOXA9) in Non- Melanoma Skin Cancer: A Clinical and Immunohistochemical study

Salama

Shoeib

Shehata

et al. 2021

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

Background: Homeobox A9 (HOXA9) has been implicated in acute myeloid leukemia and cancer breast but, little is known about HOXA9 expression in non-melanoma skin cancer (NMSC); basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (cSCC). Objective: The aim of the current work was to study HOXA9 expression in NMSC by immunohistochemical method. Patients and Methods: This case-control study included a total of 45 cases with NMSC; 15 BCC and 30 SCC beside 45 age and sex-matched normal apparently healthy controls, selected from Dermatology, Andrology & Sexually transmitted diseases (STDs) Outpatient Clinic, Menoufia University Hospital, during the period December 2018 to June 2020. Clinical evaluation and histopathological evaluation confirmed the diagnosis of SCC and BCC. Results: Expression of HOXA9 in the overlying epidermis was positive in 83.3% of SCC cases with strong intensity in 68% and nucleocytoplasmic localization in 100%. HOXA9 was positive in 60% of BCC cases with mild intensity in 77.8% and nucleocytoplasmic localization in 100%. In control sections, HOXA9 was in all sections (100%) with strong intensity in 77.8%, and nucleocytoplasmic localization in all of them 100%. Regarding tumor islands; expression of HOXA9 was positive in 26 (86.7%) cases of SCC and was positive in 2 (13.3%) of BCC cases. There was significant higher HOXA9 H-score in control skin than NMSC (p<0.001). Conclusions: It could be concluded that HOXA9 may be implicated in pathogenesis and may represent a target therapy for NMSC.

show abstract

Section: Interpretation Of Immunostaining Resultsmentioning

confidence: 99%

Expression of Homeobox A9 (HOXA9) in Non- Melanoma Skin Cancer: A Clinical and Immunohistochemical study

Salama

Shoeib

Shehata

et al. 2021

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

show abstract

“…Subsequently, due to the involvement of the TRPM8 channel in skin cancer development (49,50), AMTB, a competitive and selective TRPM8 antagonist was used in the present study. Treatment of the human melanoma cell lines with a dose of 5 µM AMTB, which is higher than the IC 50 of 0.6 µM ( 51), resulted in no significant effect on cell proliferation in any of the cell lines following 1-6 days of treatment as compared to untreated and DMSO-treated negative control groups (Fig.…”

Section: Treatment With An Antagonist Of the Trp Vanilloid-1 (Trpv1) ...mentioning

confidence: 99%

Antagonism of the transient receptor potential melastatin‑2 channel leads to targeted antitumor effects in primary human malignant melanoma cells

et al. 2022

View full text Add to dashboard Cite

Melanoma continues to be the most aggressive and devastating form of skin cancer for which the development of novel therapies is required. The present study aimed to determine the effects of antagonism of the transient receptor potential melastatin-2 (TRPM2) ion channel in primary human malignant melanoma cells. TRPM2 antagonism via use of the antifungal agent, clotrimazole, led to decreases in cell proliferation, as well as dose-dependent increases in cell death in all melanoma cell lines investigated. The targeting of TRPM2 channels was verified using TRPM2 knockdown, where treatment with TRPM2 small-interfering RNA led to similar levels of cell death in all melanoma cell lines when compared with clotrimazole treatment. Minimal effects on proliferation and cell death were observed following antagonism or knockdown of TRPM2 in non-cancerous human keratinocytes. Moreover, characteristics of TRPM2 were explored in these melanoma cells and the results demonstrated that TRPM2, localized to the plasma membrane as a non-specific ion channel in non-cancerous cells, displayed a nuclear localization in all human melanoma cell lines analyzed. Additional characterization of these melanoma cell lines confirmed that each expressed one or more established multidrug resistance genes. Results of the present study therefore indicated that antagonism of the TRPM2 channel led to antitumor effects in human melanoma cells, including those that are potentially unresponsive to current treatments due to the expression of drug resistance genes. The unique cellular localization of TRPM2 and the specificity of the antitumor effects elicited by TRPM2 antagonism suggested that TRPM2 possesses a unique role in melanoma cells. Collectively, the targeting of TRPM2 represents a potentially novel, efficacious and readily accessible treatment option for patients with melanoma.

show abstract

TRPM8 channels: A review of distribution and clinical role

Liu

Mikrani

et al. 2020

European Journal of Pharmacology

View full text Add to dashboard Cite

Increased immunoreactivity for TRPM8 in cutaneous squamous cell carcinoma

Cited by 4 publications

References 9 publications

Expression of Homeobox A9 (HOXA9) in Non- Melanoma Skin Cancer: A Clinical and Immunohistochemical study

Expression of Homeobox A9 (HOXA9) in Non- Melanoma Skin Cancer: A Clinical and Immunohistochemical study

Antagonism of the transient receptor potential melastatin‑2 channel leads to targeted antitumor effects in primary human malignant melanoma cells

TRPM8 channels: A review of distribution and clinical role

Contact Info

Product

Resources

About