Recent Trends in Biodiesel and Biogas Production

Bušić, Arijana; Kundas, Semjon; Morzak, Galina; Belskaya, Halina; Marđetko, Nenad; Šantek, Mirela Ivančić; Komes, Draženka; Novak, Srđan; Šantek, Božidar

doi:10.17113/ftb.56.02.18.5547

Cited by 93 publications

(46 citation statements)

References 124 publications

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“…The biodiesel fuel properties, including density (ρ), kinematic viscosity (η), cetane number (CN), iodine value (IV), and cold filter plugging point (CFPP), were predicted based on the FA composition (Additional file 1). During S. tonkinensis kernel development, the estimated ρ ranged from 0.856 (90 DAF) to 0.868 (50 DAF) kg/m 3 and CFPP values at kernel maturity (between 120 and 150 DAF) were 0.86 kg/m 3 , 3.40 mm 2 /s, 48.2, 118.3 g/100 g and − 9.80°C, respectively.…”

Section: Dynamic Patterns Of Oil Content and Fatty Acid Compositionmentioning

confidence: 99%

“…Biodiesel, one of the most important sources of renewable and clean energy worldwide, was first successfully produced in 1853 from vegetable oil, the first-generation biodiesel feedstock [1,2]. Second-generation biodiesel feedstock is derived from non-edible sources, such as oils from Millettia pinnata (L.) Panigrahi, Jatropha curcas L. and microalgae, solving the 'food versus fuel' dispute and decreasing production costs, especially the cost of raw materials [3]. Recently, attention has been given to woody plants with low cost and ease of cultivation as potential sources of biodiesel feedstock [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Cao

Chen

et al. 2020

BMC Plant Biol

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Background: Styrax tonkinensis (Pierre) Craib ex Hartwich has great potential as a woody biodiesel species having seed kernels with high oil content, excellent fatty acid composition and good fuel properties. However, no transcriptome information is available on the molecular regulatory mechanism of oil accumulation in developing S. tonkinensis kernels. Results: The dynamic patterns of oil content and fatty acid composition at 11 time points from 50 to 150 days after flowering (DAF) were analyzed. The percent oil content showed an up-down-up pattern, with yield and degree of unsaturation peaking on or after 140 DAF. Four time points (50, 70, 100, and 130 DAF) were selected for Illumina transcriptome sequencing. Approximately 73 million high quality clean reads were generated, and then assembled into 168,207 unigenes with a mean length of 854 bp. There were 5916 genes that were differentially expressed between different time points. These differentially expressed genes were grouped into 9 clusters based on their expression patterns. Expression patterns of a subset of 12 unigenes were confirmed by qRT-PCR. Based on their functional annotation through the Basic Local Alignment Search Tool and publicly available protein databases, specific unigenes encoding key enzymes, transmembrane transporters, and transcription factors associated with oil accumulation were determined. Three main patterns of expression were evident. Most unigenes peaked at 70 DAF, coincident with a rapid increase in oil content during kernel development. Unigenes with high expression at 50 DAF were associated with plastid formation and earlier stages of oil synthesis, including pyruvate and acetyl-CoA formation. Unigenes associated with triacylglycerol biosynthesis and oil body development peaked at 100 or 130 DAF.

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Section: Dynamic Patterns Of Oil Content and Fatty Acid Compositionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Cao

Chen

et al. 2020

BMC Plant Biol

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“…Furthermore, it is estimated that 60-80% of the biodiesel production cost stems from the cost of raw materials. All this makes use of low-cost second generation biodiesel feedstock which is very attractive alternative [49].…”

Section: Biodieselmentioning

confidence: 99%

Maize as Energy Crop

Skoufogianni¹,

Solomou²,

Charvalas³

et al. 2020

Maize - Production and Use

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Maize is the predominant raw material (together with sugar cane) for the production of bioethanol, the most common and widespread biofuel, and at the same time the predominant raw material for biogas production, with the highest yields in Europe. The advantage of maize biomass over other energy plants is the fact that biomass occurs after harvesting the seed and does not require the use of a different area for its development. The main drawback of the use of maize biomass is the negative effects of removing crop residues on fertility and the physical properties of the soil. Bioethanol's share of global biofuel production is over 94%, as many countries are replacing a portion of their fossil fuels with biofuels, according to international regulations. The choice of crops used as feedstock for the production of bioethanol is strongly associated with local climatic factors. About 60% of world bioethanol production is made with cane raw material in the Central and South American countries, with Brazil leading, while the remaining 40% from other crops with North America producing bioethanol almost exclusively from maize, and the EU uses as raw material raw starch (cereals and maize) as well as crops such as sugar beet and sweet sorghum.

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“…It is also the major by-product of biodiesel production (10 weight %). An increase in production of biodiesel (2) has led to an increase in the availability of glycerol (3). Glycerol could be an interesting substrate for fermentative production of value added products such as 1,3 propanediol, dihydroxyacetone and polyhydroxyalkanoates (4,5).…”

Section: Introductionmentioning

confidence: 99%

Insights into an alternative pathway for glycerol metabolism in a glycerol kinase deficientPseudomonas putidaKT2440

Walsh

Casey

Kenny

et al. 2019

Preprint

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16Pseudomonas putida KT2440 is known to metabolise glycerol via glycerol-3-phosphate using 17 glycerol kinase an enzyme previously described as critical for glycerol metabolism (1). 18 However, when glycerol kinase was knocked out in P. putida KT2440 it retained the ability to 19 use glycerol as the sole carbon source, albeit with a much-extended lag period and 2 fold lower 20 final biomass compared to the wild type strain. A metabolomic study identified glycerate as a 21 major and the most abundant intermediate in glycerol metabolism in this mutated strain with 22 levels 21-fold higher than wild type. Erythrose-4-phosphate was detected in the mutant strain, 23 but not in the wild type strain. Glyceraldehyde and glycraldehyde-3-phosphate were detected 24 at similar levels in the mutant strain and the wild type. Transcriptomic studies identified 191 25 genes that were more than 2-fold upregulated in the mutant compared to the wild type and 175 26 that were down regulated. The genes involved in short chain length fatty acid metabolism were 27 highly upregulated in the mutant strain. The genes encoding 3-hydroxybutyrate dehydrogenase 28 were 5.8-fold upregulated and thus the gene was cloned, expressed and purified to reveal it can 29 act on glyceraldehyde but not glycerol as a substrate. 30 31 33Glycerol is a major by-product of plant oil processing. It is also the major by-product of 34 biodiesel production (10 weight %). An increase in production of biodiesel (2) has led to an 35 increase in the availability of glycerol (3). Glycerol could be an interesting substrate for 36 fermentative production of value added products such as 1,3 propanediol, dihydroxyacetone 37 and polyhydroxyalkanoates (4,5). Polyhydroxyalkanoates (PHA) are polymers that are 38 naturally synthesized and stored in a range of microorganisms including Pseudomonads (6). 39Pseudomonas putida KT2440 is a model organism for biocatalysis and synthetic biology. 40While P. putida KT2440 can use glycerol as the sole carbon and energy source for growth, 41 glycerol metabolism in this strain has not been fully characterized. However, the pathway for 42 glycerol metabolism has been extensively studied in the opportunistic pathogen Pseudomonas 43 aeruginosa (7-9). As there is high sequence identity in the proposed glycerol metabolic 44 pathway genes in P. putida KT2440 with genes in P. aeruginosa, some information about 45 glycerol metabolism in P. putida KT2440 can be inferred from the research into P. aeruginosa 46 (10). In P. aeruginosa, the first step in glycerol metabolism is the facilitated diffusion across 47 the cytoplasmic membrane by a glycerol diffusion facilitator (GlpF). The glycerol is then 48 retained intracellularly through phosphorylation by a glycerol kinase (GlpK) to form glycerol-49 3-phosphate, which cannot diffuse back through the cytoplasmic membrane. In P. aeruginosa, 50 the genes for GlpF and GlpK are organised together on one operon (8). The glycerol-3-51 phosphate is converted to dihydroxyacetone-3-phosphate by a cytoplasmic...

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Recent Trends in Biodiesel and Biogas Production

Cited by 93 publications

References 124 publications

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Maize as Energy Crop

Insights into an alternative pathway for glycerol metabolism in a glycerol kinase deficientPseudomonas putidaKT2440

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