Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment

Abstract

High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

“…Such inter-clutch differences in the survival rates of androgenotes could result from physiological and/or molecular differences in eggs of different origins. Results of recent transcriptomic analysis of zebrafish and rainbow trout eggs confirm inter-clutch differences in expression profiles of maternal RNA (Rauwerda et al 2016;Gurgul et al 2018). Moreover, eggs from female rainbow trout whose gynogenetic offspring had the highest survival rates showed altered expression of several genes compared to eggs from other females (Ocalewicz et al 2020).…”

Preliminary Identification of Candidate Genes Related to Survival of Gynogenetic Rainbow Trout (Oncorhynchus mykiss) Based on Comparative Transcriptome Analysis

“…The eggs of different origin showing different quality may have also altered transcriptome profiles [3]. When assessing the influence of HHP on the transcriptome of the rainbow trout eggs, it was noticed that the differences in the maternal gene expression were greater between the eggs from different clutches than between the HHP-treated and the untreated eggs which originated from an individual female [12]. It was presumed that the inter-individual differences between rainbow trout females from the same broodstock had a larger influence on the egg transcriptome and the developmental ability than the HHP treatment of the eggs.…”

“…Rainbow trout utilized as gamete donors originated from the spring spawning broodstock raised in the Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, Poland. The number of dams and sires used in the research was determined to meet the requirements for the statistical analysis and it was based on the previous studies concerning the chromosomes set manipulations in fish [12,13,15]. Semen was taken from four males, pooled and kept in the plastic box.…”

“…Transcriptomic studies using the next-generation sequencing approach showed that the differences in maternal mRNA abundance in zebrafish eggs were bigger between clutches from different mothers than within the clutches [11]. In turn, in rainbow trout, greater differences in the maternal gene expression were observed between the eggs from different clutches than between the HHP-treated and untreated eggs from the same clutch [12]. Both reports indicate that differences in the expression of the maternal genes primarily result from differences between mothers.…”

Corrigendum to “Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment”

“…In the article titled “Transcriptome Analysis of Rainbow Trout ( Oncorhynchus mykiss ) Eggs Subjected to the High Hydrostatic Pressure Treatment” [1], there was an error in the first affiliation, where “Laboratory of Genomics, Department of Animal Genetics and Molecular Biology” should be changed to “Department of Animal Molecular Biology.” The corrected affiliation is shown above.…”