Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development

Upton, Rose; Clulow, Simon; Mahony, Michael; Clulow, John

doi:10.1093/conphys/coy043

Cited by 26 publications

(42 citation statements)

References 21 publications

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“…The concentration of spermatozoa in testicular macerates at ~10 [8][9] /ml is generally one to three magnitudes higher than in spermic urine (Supplementary Table 2). The high concentration, volume and quality of testicular spermatozoa has resulted in its use in most studies of Anuran spermatozoa cryopreservation until recently, and in the only two studies resulting in mature reproducing pairs [6] or sexually mature males [20]. As well, spermatozoa in suspension from testes have higher refrigerated storage potentials than those stored in intact testes, spermic urine, or in semen (Supplementary Table 2.…”

Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

“…Centrifugation is used to concentrate spermatozoa from spermic urine, to remove protein and lipid components from fresh spermatozoa suspensions [44], and post-thaw to remove cryodiluents [20].…”

Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

“…The use of centrifugation should be minimised as spermatozoa motility can be reduced by up to 50% through morphological damage [53]. Therefore, in cases where there are surplus amphibians in breeding programs [6], or males can be taken from wild populations [20], the collection of high numbers and concentrations of spermatozoa directly through testes maceration may be the preferred option.…”

Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

“…Spermatozoa are affected by varying osmolalities at various stages of processing, storage, activation, and fertilisation [1]. Spermatozoa suspensions whether created from testicular macerates [6,20], Cryptobranchid semen [47], salamander spermatophores [35] or cloacal fluid [37,39], or Anuran spermic urine [73], have high osmolalities. The greatest changes in osmolality between spermatozoa and diluents occur with the processing of spermic urine for refrigerated or cryopreserved storage, and after both refrigerated and cryopreserved storage when spermatozoa are equilibrated to recover motility [90].…”

Section: Processing Osmolalitiesmentioning

confidence: 99%

“…Post-thaw, motile spermatozoa can be used for artificial fertilization [2] and immotile spermatozoa for intracytoplasmic injection into the oocyte (ICSI; [19]). In amphibians, post-thaw spermatozoa from Anurans (frogs and toads) has resulted in reproducing pairs of Xenopus [6] and sexually mature males of tree frogs [20], and in Cuadata reproducing pairs of *Salamanders [21], and almost mature Cryptobranchids (Giant salamanders, Dale McGinnity, personal communication).…”

Section: Introductionmentioning

confidence: 99%

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Sperm collection and storage for the sustainable management of amphibian biodiversity

et al. 2019

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Current rates of biodiversity loss pose an unprecedented challenge to the conservation community, particularly with amphibians and freshwater fish as the most threatened vertebrates. An increasing number of environmental challenges, including habitat loss, pathogens, and global warming, demand a global response toward the sustainable management of ecosystems and their biodiversity. Conservation Breeding Programs (CBPs) are needed for the sustainable management of amphibian species threatened with extinction. CBPs support species survival while increasing public awareness and political influence. Current CBPs only cater for 10% of the almost 500 amphibian species in need. However, the use of sperm storage to increase efficiency and reliability, along with an increased number of CBPs, offer the potential to significantly reduce species loss. The establishment and refinement of techniques over the last two decades, for the collection and storage of amphibian spermatozoa, gives confidence for their use in CBPs and other biotechnical applications. Cryopreserved spermatozoa has produced breeding pairs of frogs and salamanders and the stage is set for Lifecycle Proof of Concept Programs that use cryopreserved sperm in CBPs along with repopulation, supplementation, and translocation programs. The application of cryopreserved sperm in CBPs, is complimentary to but separate from archival gene banking and general cell and tissue storage. However, where appropriate amphibian sperm banking should be integrated into other global biobanking projects, especially those for fish, and those that include the use of cryopreserved material for genomics and other research. Research over a broader range of amphibian species, and more uniformity in experimental methodology, is needed to inform both theory and application. Genomics is revolutionising our understanding of biological processes and increasingly guiding species conservation through the identification of evolutionary significant units as the conservation focus, and through revealing the intimate relationship between evolutionary history and sperm physiology that ultimately affects the amenability of sperm to refrigerated or frozen storage. In the present review we provide a nascent phylogenetic framework for integration with other research lines to further the potential of amphibian sperm banking.

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Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

Section: Sperm Collection and Sperm Concentrationmentioning

confidence: 99%

Section: Processing Osmolalitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sperm collection and storage for the sustainable management of amphibian biodiversity

et al. 2019

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Amphibian Assisted Reproductive Technologies: Moving from Technology to Application

Clulow

Upton

Trudeau

et al. 2019

Reproductive Sciences in Animal Conservation

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Limited effects of traffic noise on behavioural responses to conspecific mating calls in the eastern sedge frog Litoria fallax

2021

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Anthropogenic noise is a pervasive environmental feature across both urban and non-urban habitats and presents a novel challenge especially for acoustically communicating species. While it is known that some species adjust acoustic signals to communicate more effectively in noisy habitats, we know very little about how the receivers of these signals might be impacted by anthropogenic noise. Here, we investigated female and male Litoria fallax frogs’ ability to distinguish between high- and low-quality acoustic signals during the presence of background traffic noise and without. We performed a controlled behavioural experiment whereby frogs were presented with simultaneously broadcasted attractive and unattractive calls from opposing directions, once with background traffic noise and once without. We found that females in particular chose the unattractive call significantly more often (and males significantly less often) when noise was being broadcast. This indicates that anthropogenic noise potentially affects receiver responses to acoustic signals, even when calls are not acoustically masked, with potential consequences for maladaptive mating behaviours and population outcomes. Supplementary information The online version contains supplementary material available at 10.1007/s10211-021-00378-7.

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Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development

Cited by 26 publications

References 21 publications

Sperm collection and storage for the sustainable management of amphibian biodiversity

Sperm collection and storage for the sustainable management of amphibian biodiversity

Amphibian Assisted Reproductive Technologies: Moving from Technology to Application

Limited effects of traffic noise on behavioural responses to conspecific mating calls in the eastern sedge frog Litoria fallax

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