Vertical Bone Augmentation Using Three-dimensionally Printed Cap in the Rat Calvarial Partial Defect

Kim, Joong-Min; Kim, Joong-Hyun; Lee, Byeong-Han; Choi, Seok Hwa

doi:10.21873/invivo.11353

Cited by 7 publications

(7 citation statements)

References 19 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several animal models widely used for the assessment of HA substitutes on bone reconstruction (as reviewed in Reference [180]). For flat bone reconstruction, the most common is the calvarial defect model, which is frequently tested in small rodents: rat [181,182] and rabbits [183]. Long bone reconstruction uses a distraction osteogenesis model—a surgical model that creates a gap in a long bone (usually tibia), using continuous traction on the osteotomy ends.…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

View full text Add to dashboard Cite

Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

show abstract

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Histological observations indicated that the new bone does not touch the shell, thereby avoiding problems related to the separation of the samples from the shells. In addition, 3D printing technology has been successfully applied to the design of the VGBR animal model (40,49,99), which may considerably reduce the time and cost compared with traditional casting metal barrier shells. The calvaria onlay model is commonly used to compare and detect different block bone grafts.…”

Section: Discussionmentioning

confidence: 99%

“…The shell is then filled with biomaterials and fixed to the rat calvaria directly using screws via an anchor ring (30,40,43) or placed on a circular groove (27)(28)(29) that is made on the rat calvaria using a trephine under profuse irrigation with sterile saline. As the rat calvaria is thin, care should be taken not to damage the endocranium.…”

Section: Different Experimental Models Of Vertical Bone Augmentationmentioning

confidence: 99%

Animal models of vertical bone augmentation (Review)

et al. 2021

View full text Add to dashboard Cite

Vertical bone augmentation is an important challenge in dental implantology. Existing vertical bone augmentation techniques, along with bone grafting materials, have achieved certain clinical progress but continue to have numerous limitations. In order to evaluate the possibility of using biomaterials to develop bone substitutes, medical devices and/or new bone grafting techniques for vertical bone augmentation, it is essential to establish clinically relevant animal models to investigate their biocompatibility, mechanical properties, applicability and safety. The present review discusses recent animal experiments related to vertical bone augmentation. In addition, surgical protocols for establishing relevant preclinical models with various animal species were reviewed. The present study aims to provide guidance for selecting experimental animal models of vertical bone augmentation. Contents 1. Introduction 2. Different experimental models of vertical bone augmentation 3. Discussion 4. Conclusion

show abstract

“…The calvarial bone defect rat model was established as described in previous studies [ 22 , 23 ] with a few modifications. Briefly, the rats were intraperitoneally anaesthetized with alpha chloralose (55 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors

Zhou

Zhao

et al. 2020

Med Sci Monit

View full text Add to dashboard Cite

Background Bone tissue engineering has been proven to be an appropriate approach for treating bone defects. This study aimed to investigate the effects and mechanism of a composite tissue engineered bone material consisting of bone mesenchymal stem cells (BMSCs), bone morphogenetic protein (BMP9) gene lentiviral vector, and P3HB4HB thermogel (BMSCs-LV-BMP9-P3HB4HB) on calvarial skull defects in rats. Material/Methods LV-BMP9 viral vector was structured and infected to BMSCs-P3HB4HB composite scaffold, which was named as BMSCs-P3HB4HB composite bone repair material. Adipogenic differentiation was determined by oil-red O (ORO) and alkaline phosphatase (ALP) staining. Osteogenic differentiation was measured using Alizarin red staining. Cell viability was examined using Cell-Counting Kit-8 (CCK-8) assay. Protein expression of osteogenic factors, including BMP9, runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), and osterix (OSX), was detected with Western blot assay and immunohistochemistry. mRNA of these osteogenic factors was examined by RT-PCR. Histological changes were examined with hematoxylin and eosin (H&E) and Masson’s trichrome staining. Bone repair was measured using micro-computed tomography (micro-CT). Results BMSCs and LV-BMP9-infected BMSCs demonstrated adipogenic and osteogenic differentiation potential. BMSCs-P3HB4HB scaffold demonstrated good cell-tissue compatibility. BMSCs-LV-BMP9-P3HB4HB exhibited significantly higher osteogenic ability and cell viability of BMSCs compared to BMSCs-LV-P3HB4HB ( p <0.05). BMSCs-LV-BMP9-P3HB4HB significantly promoted osteogenic factors (RUNX2, OCN, OPN, and OSX) expression compared to the BMSCs-LV-P3HB4HB group ( p <0.05) in both BMSCs and in calvarial defect rats. BMSCs-LV-BMP9-P3HB4HB demonstrated stronger repair ability. BMSCs-LV-BMP9-P3HB4HB significantly alleviated pathological injury and increased collagen fiber production compared to the BMSCs-LV-P3HB4HB group ( p <0.05). Conclusions BMSCs-LV-BMP9-P3HB4HB composite bone repair material can effectively repair injured skull tissues of calvarial defect rats through triggering osteogenic factors expression. The present generated bone repair material may have applications in tissue engineering in regeneration of bone defects.

show abstract

Vertical Bone Augmentation Using Three-dimensionally Printed Cap in the Rat Calvarial Partial Defect

Abstract: The results clearly showed that this system was successful for VBA in a research animal model.

Cited by 7 publications

References 19 publications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Animal models of vertical bone augmentation (Review)

A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors

Contact Info

Product

Resources

About