Interference of Thalassemias on Hemoglobin A1c Measurement by Ion-Exchange High-Performance Liquid Chromatography Method Tosoh HLC-723 G8

Chao, Yu; Wan, Zemin; Wu, Xiaobin; Qiu, Feng; Wang, Yunxiu; Ke, Peifeng; Wu, Xinzhong; Xu, Jianhua; Zhang, Junhua; Huang, Xianzhang

doi:10.7754/clin.lab.2018.180119

Cited by 2 publications

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“…Automated high-performance liquid chromatography (HPLC) methods are the gold standards for measuring HbA1c levels accurately. However, they are vulnerable to unstable and/or abnormal hemoglobin variants that may interfere with the measures. − These methods produce discordant levels, which led the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to create a coalition aimed at standardizing HbA1c determination. One of the coalition’s aims was to establish a reference method that has been exhaustively investigated and shows negligible inaccuracy and imprecision .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

et al. 2022

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Data-independent acquisition (DIA) allows comprehensive proteome coverage, while it also potentially works as a unified protocol to determine a multitude of proteins found in blood. Because of its high specificity, mass spectrometry may greatly reduce the interference observed in other assays to evaluate blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing in a platform to assess clinical markers. As a proof of concept, we evaluated two hemoglobin-related biomarkers: the glycated hemoglobin (HbA1c) and hemoglobin (Hb) variants. HbA1c by DIA showed good correlation with the reference method, but method imprecision did not meet the quality requirement for this biomarker. We developed a strategy to identify Hb variants based on a customized database combined with a workflow for DIA data extraction and rigorous peptide evaluation. Data are available via ProteomeXchange with identifier PXD029918.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

et al. 2022

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Analytical interference of 33 different hemoglobin variants on HbA1c measurements comparing high-performance liquid chromatography with whole blood enzymatic assay: A multi-center study

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Kreutzig

et al. 2022

Clinica Chimica Acta

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Interference of Thalassemias on Hemoglobin A1c Measurement by Ion-Exchange High-Performance Liquid Chromatography Method Tosoh HLC-723 G8

Abstract: Thalassemia could directly affect the measurement of HbA1c using the IE-HPLC method Tosoh G8 and the clinical laboratorial staff should pay close attention.

Cited by 2 publications

References 0 publications

Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

Analytical interference of 33 different hemoglobin variants on HbA1c measurements comparing high-performance liquid chromatography with whole blood enzymatic assay: A multi-center study

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