Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

Lima, Debora A; Schuch, Rodrigo Andrade; Salgueiro, Jéssica Silva; Pintão, Maria Carolina; Carvalho, Valdemir Melechco

doi:10.1021/acs.jproteome.1c00892

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Targeted determination of hemoglobin (Hb), Hb variants and Hb disorders using LC-MS from DBS has, since the first publication [4], been described often. In the time span focused on in this review only few MS-based Hb-specific studies from DBS were reported [46][47][48][49][50][51][52]. Weisinger et at.…”

Section: Targeted Protein Determination Without Affinity Clean-upmentioning

confidence: 99%

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Reubsaet,

Halvorsen

2024

J of Separation Science

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Determination of proteins from dried matrix spots using MS is an expanding research area. Mainly, the collected dried matrix sample is whole blood from a finger or heal prick, resulting in dried blood spots. However as other matrices such as plasma, serum, urine, and tear fluid also can be collected in this way, the term dried matrix spot is used as an overarching term. In this review, the focus is on advancements in the field made from 2017 up to 2023. In the first part reviews concerning the subject are discussed. After this, advancements made for clinical purposes are highlighted. Both targeted protein analyses, with and without the use of affinity extractions, as well as untargeted, global proteomic approaches are discussed.In the last part, both methodological advancements are being reviewed as well as the possibility to integrate sample preparation steps during the sample handling. The focus, of this so‐called smart sampling, is on the incorporation of cell separation, proteolysis, and antibody‐based affinity capture.

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Section: Targeted Protein Determination Without Affinity Clean-upmentioning

confidence: 99%

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Reubsaet,

Halvorsen

2024

J of Separation Science

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“…The conclusion was drawn that HRMS without any chromatographic separation was a fast, cheap, and highly specific way to differentiate between many Hb variants [24]. Also the use of DIA in nano-LC HRMS made differentiation between different Hb variants possible in dried blood from volumetric absorptive microsampling [56].…”

Section: 21mentioning

confidence: 99%

Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry

Skogvold

Rootwelt

Reubsaet

et al. 2023

J of Separation Science

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Dried blood spot samples are simple to prepare and transport, enabling safe and accessible diagnostics, both locally and globally. We review dried blood spot samples for clinical analysis, focusing on liquid chromatography‐mass spectrometry as a versatile measurement tool for these samples. Dried blood spot samples can provide information for, for example, metabolomics, xenobiotic analysis, and proteomics. Targeted analyses of small molecules are the main application of dried blood spot samples and liquid chromatography‐mass spectrometry, but emerging applications include untargeted metabolomics and proteomics. Applications are highly varied, including analyses related to newborn screening, diagnostics and monitoring of disease progression and treatment effects of virtually any disease, as well as studies into the physiology and effects of diet, exercise, xenobiotics, and doping. A range of dried blood spot products and methods are available, and applied liquid chromatography‐mass spectrometry instrumentation is varied with regard to liquid chromatography column formats and selectivity. In addition, novel approaches such as on‐paper sample preparation (e.g., selective trapping of analytes with paper‐immobilized antibodies) are described. We focus on research papers published in the last 5 years.

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Remote HbA_1c testing via microsampling: fit for purpose?

Verougstraete

Stove

2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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The collection of capillary blood microsamples via finger-prick has several advantages over traditional blood collection. It is considered convenient and more patient-centric, enabling collection of the sample by the patient at her/his home with subsequent analysis in the lab following postal shipment. Determination of the diabetes biomarker HbA1c in self-collected microsamples to remotely monitor diabetes patients seems to be a very promising option which could eventually lead to better treatment adaptations and disease control. This is especially convenient/relevant for patients living in areas where venipuncture is impractical, or to support virtual consultations using telemedicine. Over the years, a substantial numbers of reports on HbA1c and microsampling have been published. However, the heterogeneity of the applied study designs and data evaluation is remarkable. This review provides a general and critical overview of these papers, along with specific points of attention that should be dealt with when aiming at implementing microsampling for reliable HbA1c determination. We focus on the used (dried) blood microsampling techniques, collection conditions, stability of the microsamples, sample extraction, analytical methods, method validation, correlation studies with conventional venous blood samples and patient satisfaction. Lastly, the possibility of using liquid instead of dried blood microsamples is discussed. Liquid blood microsampling is expected to have similar advantages as dried blood microsampling and several studies suggest it to be a suitable approach to collect samples remotely for subsequent HbA1c analysis in the lab.

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Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

Cited by 3 publications

References 46 publications

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry

Remote HbA_1c testing via microsampling: fit for purpose?

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Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers

Cited by 3 publications

References 46 publications

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Advancements in clinical approaches, analytical methods, and smart sampling for LC–MS‐based protein determination from dried matrix spots

Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry

Remote HbA1c testing via microsampling: fit for purpose?

Contact Info

Product

Resources

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Remote HbA_1c testing via microsampling: fit for purpose?