25-Hydroxyvitamin D Testing

Garg, Uttam

doi:10.1016/j.cll.2018.05.007

Cited by 28 publications

(8 citation statements)

References 35 publications

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“…Nikooyeb et al [26] analyzed 275 general population serum samples using different methods, and argued that although the chromatographic techniques are the gold standard for the laboratory test determination of vitamin D, the results are comparable, since there are no major differences among the techniques. However, other authors such as Garg et al [52] stated that although the differences in results obtained by the various vitamin D analytical methods have been reduced in recent years, it is advisable to use chromatographic techniques until full harmonization of the analytical methods for vitamin D is achieved. To date, the immunochemical methods have not been able to match the precision and specificity of the chromatographic techniques [53].…”

Section: Discussionmentioning

confidence: 99%

Response of Vitamin D after Magnesium Intervention in a Postmenopausal Population from the Province of Granada, Spain

et al. 2020

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Menopause is a stage of hormonal imbalance in women which, in addition to other physiopathological consequences, poses a risk of deficiency of key micronutrients such as magnesium and vitamin D. A study was made of the influence of a magnesium intervention upon vitamin D status in a postmenopausal population from the province of Granada (Spain). Fifty-two healthy postmenopausal women between 44–76 years of age were included. Two randomized groups—placebo and magnesium (500 mg/day)—were treated during eight weeks. Nutrient intake was assessed using questionnaires based on 72-h recall. Vitamin D was analyzed by liquid chromatography—tandem mass spectrometry. Baseline vitamin D proved deficient in over 80% of the subjects. The administration of magnesium resulted in significantly increased vitamin D levels in the intervention group versus the controls (p < 0.05). Magnesium supplementation improved vitamin D status in the studied postmenopausal women.

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Section: Discussionmentioning

confidence: 99%

Response of Vitamin D after Magnesium Intervention in a Postmenopausal Population from the Province of Granada, Spain

et al. 2020

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“…The most common method of assay for 25-OH-D is FDA-approved immunoassay [1], but recovery of 25-OH-D2 is generally lower than that of 25-OH-D3, and the degree to which these assays recognize 25-OH-D2 is highly variable [2,3]. Therefore, it is important to verify how individual assays perform to enable accurate determination of Vitamin D status in this supplemented population.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3

Garnett

Rajapakshe

et al. 2019

Practical Laboratory Medicine

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Objectives Assessment of Vitamin D status by measurement of 25-Hydroxyvitamin D (25-OH-D) is widely performed by immunoassay. Yet, the ability of these assays to detect Vitamin D2 (as 25-OH-D2) or Vitamin D3 (as 25-OH-D3) varies. It is important to recognize the ability of an assay to quantitate either form of 25-OH-D to evaluate Vitamin D status of supplemented patients. We evaluated detection of 25-OH-D2 and 25-OH-D3 by two assays in our medical center. Design and methods The Abbott Architect i1000 SR 25-OH Vitamin D assay and Roche Cobas 8000 Vitamin D assay were compared for their recovery of 25-OH-D2 or D3 from spiked serum samples. Samples with known endogenous concentrations of 25-OH-D2 or D3 by LC-MS/MS were also measured to calculate bias between our assays and LC-MS/MS. Results Recovery of 25-OH-D3 in spiked samples was similar by Architect (84–87%) and Cobas (90%). Recovery of 25-OH-D2 was lower than 25-OH-D3, and was poorer by Architect (37–40%) than by Cobas (69–71%). In measurement of samples with known 25-OH-D concentrations, performance of Architect and Cobas assays was similar for 25-OH-D3. However, at concentrations >50 nmol/L 25-OH-D2, the Architect assay exhibited large average negative bias (−27%). Conclusions While the Architect and Cobas assays performed similarly in detection of 25-OH-D3, the Architect assay was significantly poorer at detecting 25-OH-D2 than Cobas, with poorer recovery and significant negative bias at higher concentrations of 25-OH-D2. This agrees with other studies, and indicates that caution should be used in interpreting Architect 25-OH-D results in patients supplemented with Vitamin D2.

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“…There are many extensive reviews comparing and discussing the different methods for the quantification of vitamin D metabolites, with many focusing particularly on LC-MS/MS methods (in humans) and the particular challenges of quantifying each metabolite in detail [33,[248][249][250][251][252][253]. The author directs the reader to this referenced literature for detailed comparisons of reported vitamin D LC-MS/MS methods.…”

Section: Analytical Challenges In Profiling Vitamin D Metabolites In mentioning

confidence: 99%

Vitamin D Metabolism and Profiling in Veterinary Species

2020

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The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods.

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25-Hydroxyvitamin D Testing

Cited by 28 publications

References 35 publications

Response of Vitamin D after Magnesium Intervention in a Postmenopausal Population from the Province of Granada, Spain

Response of Vitamin D after Magnesium Intervention in a Postmenopausal Population from the Province of Granada, Spain

Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3

Vitamin D Metabolism and Profiling in Veterinary Species

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