Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3

Garnett, Emily; Li, Jieli; Rajapakshe, Deepthi; Tam, Estella; Meng, Qing H.; Devaraj, Sridevi

doi:10.1016/j.plabm.2019.e00130

Cited by 8 publications

(5 citation statements)

References 6 publications

(6 reference statements)

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“…For the Abbott assay, they observed a positive bias (18%) for SRM 972 level 1 and negative bias (12%) for level 3, which has a high concentration of 25(OH)D 2 ; these results are similar to results observed in this study. Garnett et al [29] investigated the efficacy of the Abbott and the Roche assays for recovery of 25(OH)D 2 and 25(OH)D 3 and found that the two assays had similar response for 25(OH)D 3 but the Abbott assay was significantly negatively biased compared to ID LC-MS/MS assay for 25(OH)D 2 , which is in agreement with the results of this study. Wyness and Straseski [40] evaluated six assays including the Abbott assay and found that the Abbott and Roche assays underestimated 25(OH)D 2 by about 25%.…”

Section: Performance Of Individual Ligand Binding Assayssupporting

confidence: 89%

“…Annema et al [28] evaluated the Abbott re-standardized Architect assay and found that the assay slightly underestimates 25(OH)D levels at low concentrations. Garnett et al [29] evaluated the Abbott Architect and Roche Cobas assays for their recoveries of 25(OH)D 2 and 25(OH)D 3 and concluded that caution should be used in interpreting results using the Abbott assay in patients supplemented with vitamin D 2 . Lim et al [30] evaluated the performance of three ligand binding assays for serum total 25(OH)D, with particular emphasis on the Abbott assay, and compared results with an ID LC-MS/MS method.…”

Section: Introductionmentioning

confidence: 99%

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Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 — Part 2 ligand binding assays — impact of 25-hydroxyvitamin D2 and 24R,25-dihydroxyvitamin D3 on assay performance

et al. 2021

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An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D 2 [25(OH)D 2 ], 25-hydroxyvitamin D 3 [25(OH)D 3 ], 3-epi-25-hydroxyvitamin D 3 [3-epi-25(OH)D 3 ], and 24R,25-dihydroxyvitamin D 3 [24R,25(OH) 2 D 3 ] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 50% of the ligand binding assays achieved the VDSP criterion of mean % bias ≤ |± 5%|. For the 13 unique ligand binding assays evaluated in Published in the topical collection celebrating ABCs 20th Anniversary.

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Section: Performance Of Individual Ligand Binding Assayssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 — Part 2 ligand binding assays — impact of 25-hydroxyvitamin D2 and 24R,25-dihydroxyvitamin D3 on assay performance

et al. 2021

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“…Evaluation of VD status by 25(OH)D measurement is usually carried out by immunoassay. 78 Enzyme-linked immunosorbent assay (ELISA)…”

Section: Immunoassaymentioning

confidence: 99%

Vitamin D metabolites and analytical challenges

et al. 2023

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“…Blood samples for the assessment of vitamin D levels were prospectively collected from all BCC patients and further transferred to the certified E. Gulbis Laboratory Ltd (LATAK accreditation ISO 15189). A conventional chemiluminescence immunoassay using the Cobas 8000 analyzer (Roche, Basel, Switzerland) was performed to measure a total vitamin D serum level [36][37][38].…”

Section: Assays Used For the Detection Of Serum Vitamin D Levelsmentioning

confidence: 99%

Serum Vitamin D Levels Explored in the Latvian Cohort of Patients with Basal Cell Carcinoma Linked to the Sonic Hedgehog and Vitamin D Binding Protein Cutaneous Tissue Indices

Moisejenko-Goluboviča

Groma

Svirskis

et al. 2022

Nutrients

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Ultraviolet radiation is known as one of the major contributors to skin malignancies, including basal cell carcinoma (BCC), which is the most common type of skin cancer. It is a heterogeneous tumor, which presents with various types that are stratified into low- and high-risk tumors. Sunlight is important for overall health and vitamin D synthesis in the skin, whereas deviations from the optimal level of vitamin D are shown to be associated with the risk of the development of BCC. The accumulating evidence suggests the ability of vitamin D to antagonize the Sonic Hedgehog (SHH) signaling, the key tumor pathway, and play a protective role in the development of BCC. Additionally, a vitamin D binding protein (DBP) is shown to be implicated in the complex regulation of vitamin D. Here, we aimed to explore serum vitamin D in patients with different primary and recurrent BCC of the head and neck and investigate cutaneous DBP and SHH indices, confirmed immunohistochemically in these subjects. According to the results, 94.9% of the Latvian cohort of BCC patients were found to be deficient in vitamin D. No significant differences in serum vitamin D levels were found between genders, primary and recurrent tumors, and different types of BCC. Serum vitamin D was inversely associated with tumor size. Susceptible male individuals with low blood vitamin D levels were recognized at risk of developing aggressive and recurrent BCC confirmed by the use of hierarchical clustering analysis. In smaller tumors with a favorable course, such as superficial and nodular BCC, the association between high DBP and low SHH tissue expression was found, providing supportive evidence of the existence of a link between vitamin D, proteins involved in its metabolism, as exemplified by the DBP and SHH signaling pathway. The assumption of a deficiency in the protective effect of vitamin D in patients with high-risk BCCs was proposed in low DBP and high SHH tissue indices. New extensions to existing knowledge and characterization of the BCC signaling pathways and their cross-talk with vitamin D are warranted when searching for a preferential effect of vitamin D on skin cancer.

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Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3

Cited by 8 publications

References 6 publications

Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 — Part 2 ligand binding assays — impact of 25-hydroxyvitamin D2 and 24R,25-dihydroxyvitamin D3 on assay performance

Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 — Part 2 ligand binding assays — impact of 25-hydroxyvitamin D2 and 24R,25-dihydroxyvitamin D3 on assay performance

Vitamin D metabolites and analytical challenges

Serum Vitamin D Levels Explored in the Latvian Cohort of Patients with Basal Cell Carcinoma Linked to the Sonic Hedgehog and Vitamin D Binding Protein Cutaneous Tissue Indices

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