Trichoderma harzianum diminished oxidative stress caused by 2,4- dichlorophenoxyacetic acid (2,4-D) in wheat, with insights from lipidomics

Bernat, Przemysław; Nykiel-Szymańska, Justyna; Gajewska, Ewa; Różalska, Sylwia; Stolarek, Paulina; Dackowa, Julia; Słaba, Mirosława

doi:10.1016/j.jplph.2018.07.010

Cited by 20 publications

(15 citation statements)

References 28 publications

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“…Trichoderma species are well known biocontrol agents that promote plant growth. In the study by Bernat et al, the Trichoderma strain was able to reduce the impacts of herbicides on shoots and roots [ 5 ]. Usage of its metabolites alone in the presence of the herbicide was not sufficient for root development to a degree comparable to that caused by the fungus.…”

Section: Resultsmentioning

confidence: 99%

“…According to our knowledge, T. harzianum can improve the germination of wheat kernels and reduce the toxic effects caused by the herbicide 2,4-D [ 5 ]. This strain is used as a commercial bioactive agent due to its ability to increase plant resistance against abiotic stresses and protect plants against pathogens [ 6 ] by competing with them for ingredients and even by parasitizing them [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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Wheat is a critically important crop. The application of fungi, such as Trichoderma harzianum, to protect and improve crop yields could become an alternative solution to synthetic chemicals. However, the interaction between the fungus and wheat in the presence of stress factors at the molecular level has not been fully elucidated. In the present work, we exposed germinating seeds of wheat (Triticum aestivum) to the plant pathogen Fusarium culmorum and the popular herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of T. harzianum or its extracellular metabolites. Then, the harvested roots and shoots were analyzed using spectrometry, 2D-PAGE, and MALDI-TOF/MS techniques. Although F. culmorum and 2,4-D were found to disturb seed germination and the chlorophyll content, T. harzianum partly alleviated these negative effects and reduced the synthesis of zearalenone by F. culmorum. Moreover, T. harzianum decreased the activity of oxidoreduction enzymes (CAT and SOD) and the contents of the oxylipins 9-Hode, 13-Hode, and 13-Hotre induced by stress factors. Under the influence of various growth conditions, changes were observed in over 40 proteins from the wheat roots. Higher volumes of proteins and enzymes performing oxidoreductive functions, such as catalase, ascorbate peroxidase, cytochrome C peroxidase, and Cu/Zn superoxide dismutase, were found in the Fusarium-inoculated and 2,4-D-treated wheat roots. Additionally, observation of the level of 12-oxo-phytodienoic acid reductase involved in the oxylipin signaling pathway in wheat showed an increase. Trichoderma and its metabolites present in the system leveled out the mentioned proteins to the control volumes. Among the 30 proteins examined in the shoots, the expression of the proteins involved in photosynthesis and oxidative stress response was found to be induced in the presence of the herbicide and the pathogen. In summary, these proteomic and metabolomic studies confirmed that the presence of T. harzianum results in the alleviation of oxidative stress in wheat induced by 2,4-D or F. culmorum.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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“…This is similar to previous reports, where the antioxidant potential increased in mustard and tomato aer Trichoderma inoculation. 58,59 Herein, we observed that compared to the untreated plants, NO 3 À -fed (T2) tobacco showed greater SOD activity (13.9 EU g À1 FW) than the NH 4 + -fed plants (T4; 11.8 EU g À1 FW) at 6-24 hpi aer Xoo challenged (Fig. 4A).…”

Section: Induction Of Antioxidant Enzymes Activities and Gene Expressmentioning

confidence: 79%

Trichoderma asperellumT42 induces local defense againstXanthomonas oryzaepv.oryzaeunder nitrate and ammonium nutrients in tobacco

et al. 2019

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“…Beneficial effects of Trichoderma spp. on plant development are reported in rice [76], melon [69], sorghum [70], and wheat [71,72]. In soybean crop, growth promotion is related to the synthesis of 1-aminocyclopropane-1-carboxylate deaminase, indole acetic acid (IAA), siderophores, and biological nitrogen fixation [73,74].…”

Section: Sanity Of Soybean Seeds Treated With Trichoderma Sppmentioning

confidence: 99%

Trichodermaas a Biocontrol Agent againstSclerotiniaStem Rot or White Mold on Soybeans in Brazil: Usage and Technology

Juliatti¹,

Rezende²,

Juliatti³

et al. 2019

Trichoderma - The Most Widely Used Fungicide

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Biological control agents are alternatives to chemical pesticides in the management of plant diseases. Currently, hundreds of bioproducts are commercially available in international market varying mainly in antagonistic microorganisms and formulation. We screened four Trichoderma-based products as to their efficacy in controlling Sclerotinia stem rot (SSR) under protected and field environments and their effect on soybean seeds' sanity and physiological qualities. We also tested application technologies through seed microbiolization and foliar spraying to deliver the microorganisms, and their compatibility with chemical fungicides. In vitro assays showed that all Trichoderma strains were antagonistic to S. sclerotiorum evidencing hyperparasitic activity. Moreover, the bioproducts reduced fungi incidence on soybean seeds, promoted faster seedling emergence and did not hamper seeds' vigor. Increases of 14 and 37% were registered for root length and shoot fresh weight over that of the untreated control indicating potential application of the bioproducts as soybean growth promoters. Thiophanate-methyl and procymidone were the most compatible, without drastically affecting spore germination or mycelium growth. Under field conditions, all Trichoderma strains reduced SSR incidence and increased soybean grain yield. Formulation interferes on bioproducts' viability and efficacy deserving special attention upon development.

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Trichoderma harzianum diminished oxidative stress caused by 2,4- dichlorophenoxyacetic acid (2,4-D) in wheat, with insights from lipidomics

Cited by 20 publications

References 28 publications

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Trichoderma asperellumT42 induces local defense againstXanthomonas oryzaepv.oryzaeunder nitrate and ammonium nutrients in tobacco

Trichodermaas a Biocontrol Agent againstSclerotiniaStem Rot or White Mold on Soybeans in Brazil: Usage and Technology

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