Functional characterization of persimmon β-galactosidase gene DkGAL1 in tomato reveals cell wall modification related to fruit ripening and radicle elongation

Ban, Qiuyan; Han, Ye; He, Yiheng; Jin, Mijing; Han, Shoukun; Suo, Jiangtao; Rao, Jingping

doi:10.1016/j.plantsci.2018.05.014

Cited by 35 publications

(8 citation statements)

References 67 publications

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“…As expected, the up-regulation of markers of SA (i.e., chitinase and1,3-B glucanase) and JA pathways (i e., metallocarboxypeptidase inhibitor and, Type I serine protease inhibitor; Supplementary Table 2A) were also observed. Overall, these data show that Trichoderma colonization of tomato plants positively affects several metabolic pathways, consistently with previous observations in tomato and other plant species (Alexandru et al, 2013; Mohapatra and Mittra, 2016; Ban et al, 2018). Since phenylpropanoids contribute to plant defenses (both direct and indirect) to insect herbivores, genes encoding for key-enzymes associated with phenylpropanoids biosynthesis were searched by using DEGs in a query to a KEGG database; in addition, a manual curation to enrich the aforementioned list was performed.…”

Section: Resultssupporting

confidence: 91%

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Coppola¹,

et al. 2019

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Beneficial fungi in the genus Trichoderma are among the most widespread biocontrol agents of plant pathogens. Their role in triggering plant defenses against pathogens has been intensely investigated, while, in contrast, very limited information is available on induced barriers active against insects. The growing experimental evidence on this latter topic looks promising, and paves the way toward the development of Trichoderma strains and/or consortia active against multiple targets. However, the predictability and reproducibility of the effects that these beneficial fungi is still somewhat limited by the lack of an in-depth understanding of the molecular mechanisms underlying the specificity of their interaction with different crop varieties, and on how the environmental factors modulate this interaction. To fill this research gap, here we studied the transcriptome changes in tomato plants (cultivar “Dwarf San Marzano”) induced by Trichoderma harzianum (strain T22) colonization and subsequent infestation by the aphid Macrosiphum euphorbiae . A wide transcriptome reprogramming, related to metabolic processes, regulation of gene expression and defense responses, was induced both by separate experimental treatments, which showed a synergistic interaction when concurrently applied. The most evident expression changes of defense genes were associated with the multitrophic interaction Trichoderma -tomato-aphid. Early and late genes involved in direct defense against insects were induced (i.e., peroxidase, GST, kinases and polyphenol oxidase, miraculin, chitinase ), along with indirect defense genes, such as sesquiterpene synthase and geranylgeranyl phosphate synthase . Targeted and untargeted semi-polar metabolome analysis revealed a wide metabolome alteration showing an increased accumulation of isoprenoids in Trichoderma treated plants. The wide array of transcriptomic and metabolomics changes nicely fit with the higher mortality of aphids when feeding on Trichoderma treated plants, herein reported, and with the previously observed attractiveness of these latter toward the aphid parasitoid Aphidius ervi . Moreover, Trichoderma treated plants showed the over-expression of transcripts coding for several families of defense-related transcription factors (bZIP, MYB, NAC, AP2-ERF, WRKY), suggesting that the fungus contributes to the priming of plant responses against pest insects. Collectively, our data indicate that Trichoderma treatment of tomato plants induces transcriptomic and metabolomic changes, which underpin both direct and indirect defense responses.

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Section: Resultssupporting

confidence: 91%

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Coppola¹,

et al. 2019

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“…Fruit growth is a result of rapid cell division and cell elongation until the fruit gains its maximum size and starts ripening [41]. Cyclins are well known for regulating cell cycle [52].…”

Section: Discussionmentioning

confidence: 99%

“…Rapid cell elongation occurs during all plant growth stages [40]. The modification of the plant cell wall is highly required for the release of wall stress and a decrease in rigidity to allow cell elongation [12,41,42]. We identified 19 cell wall metabolism-related genes showing significant differential expression.…”

Section: Cell Wall Metabolismmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Two Bottle Gourd Accessions Differing in Fruit Size

Zhang

Tan

Zhang

et al. 2020

Genes

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The bottle gourd (Lagenaria siceraria) is an important horticultural and medicinal crop with high nutritional value. This study aimed at examining the molecular regulation of fruit size in bottle gourd. We performed transcriptome sequencing of two bottle gourd cultivars differing in their fruit size. The average fruit length and weight of the cultivar Hang (39.48 cm/624.4 g) were higher than those of the cultivar USA (10.34 cm/152.8 g) at maturity. Transcriptome sequencing and assembly resulted in 89,347 unigenes. A total of 1250 differentially expressed genes (DEG) were found between the two cultivars, including 422 upregulated genes and 828 downregulated genes in Hang as compared to USA. Genes related to cell wall metabolism, phytohormones, cell cycle, and cell division showed significant differential expression between the two cultivars. DEGs encoding transcription factors (TF) from nine TF families were also identified. The ethylene response factor family was the most enriched among these families. Our study provides a basis for further investigations of the molecular regulation of fruit size in bottle gourd.

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“…β-galactosidase in the cell wall was involved in germination and seedling growth of chickpea (Hernández-Nistal et al 2014). β-galactosidase gene DkGAL1 revealed that cell wall modification was related to fruit ripening in tomato (Ban et al 2018). β-galactosidase was also detected in fungi, and most of the research was on the characteristics of β-galactosidase (Park & Oh 2009;Isobe et al 2013).…”

Section: Go Terms Involved After Chaetoviridin a Treatmentmentioning

confidence: 99%

Transcriptome reveals the molecular mechanism of chaetoviridin A inhibiting the spore germination of Verticillium dahliae

et al. 2023

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The pathogen Verticillium dahliae causes Verticillium wilt in a number of crops, including cotton Verticillium wilt. Chaetoviridin A, a secondary metabolite of Chaetomium globosum, significantly inhibits the growth of V. dahliae. Spore germination is a major part of the disease cycle. However, the molecular mechanism of chaetoviridin A inhibiting spore germination of V. dahliae is unknown. In this work, we found that chaetoviridin A significantly inhibited spore germination of V. dahliae. Transcriptome analysis showed that DEGs were enriched in linolenic acid metabolism, alpha-Linolenic acid metabolism, Arachidonic acid metabolism and Purine metabolism pathways at 1 h, which were related to cell membrane. At 3 h, DEGs were enriched in the pathways of galactose metabolism, diterpenoid biosynthesis, cysteine and methionine metabolism, and starch and sucrose metabolism, which were mainly related to amino acid metabolism and sugar metabolism. Several genes related to glucose metabolism were identified, mainly including Glucagon endo-1,3-alpha-glucosidase agn1, glucoamylase, maltose O-acetyltransferase, and beta-galactosidase. Stress resistance gene PAL, detoxification gene P450 2C31 and ABA receptor were also down- regulated. These genes may be related to spore germination. These results provide a theoretical basis for chaetoviridin A to control fungal diseases.

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Functional characterization of persimmon β-galactosidase gene DkGAL1 in tomato reveals cell wall modification related to fruit ripening and radicle elongation

Cited by 35 publications

References 67 publications

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Transcriptome and Metabolome Reprogramming in Tomato Plants by Trichoderma harzianum strain T22 Primes and Enhances Defense Responses Against Aphids

Comparative Transcriptomic Analysis of Two Bottle Gourd Accessions Differing in Fruit Size

Transcriptome reveals the molecular mechanism of chaetoviridin A inhibiting the spore germination of Verticillium dahliae

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