Implications of maintenance of mother–bud neck size in diverse vital processes of Saccharomyces cerevisiae

Kubo, Kosuke; Okada, Hiroki; Shimamoto, Takuya; Kimori, Yoshitaka; Mizunuma, Masaki; Bi, Erfei; Ohnuki, Shinsuke; Ohya, Yoshikazu

doi:10.1007/s00294-018-0872-2

Cited by 7 publications

(6 citation statements)

References 63 publications

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“…Consistent with a recent study 19 , we observe a slight increase of septin ring diameter during the cell cycle ( Fig. 1d and Supplementary Fig.…”

Section: Resultssupporting

confidence: 93%

“…Together with the observation that in fission yeast the size of the Cdc42-GTP cluster is set by the local radius of cell curvature 18 -and therefore increases with cell size-this suggests a role of Cdc42-GTP cluster size homeostasis in controlling the septin ring diameter. Finally, analysis of deletion mutants with increased bud-neck width revealed a potentially causal correlation of cell size and neck width 19 .…”

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confidence: 99%

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Cell size sets the diameter of the budding yeast contractile ring

et al. 2020

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The formation and maintenance of subcellular structures and organelles with a well-defined size is a key requirement for cell function, yet our understanding of the underlying size control mechanisms is limited. While budding yeast cell polarization and subsequent assembly of a septin ring at the site of bud formation has been successfully used as a model for biological self-assembly processes, the mechanisms that set the size of the septin ring at the bud neck are unknown. Here, we use live-cell imaging and genetic manipulation of cell volume to show that the septin ring diameter increases with cell volume. This cell-volume-dependence largely accounts for modulations of ring size due to changes in ploidy and genetic manipulation of cell polarization. Our findings suggest that the ring diameter is set through the dynamic interplay of septin recruitment and Cdc42 polarization, establishing it as a model for size homeostasis of self-assembling organelles.

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“…Consistent with a recent study 19 , we observe a slight increase of septin ring diameter during the cell cycle ( Fig. 1d and Supplementary Fig.…”

Section: Resultssupporting

confidence: 93%

mentioning

confidence: 99%

Cell size sets the diameter of the budding yeast contractile ring

et al. 2020

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“…However, upon mild sonication (to reduce clumps for imaging), neither mutant showed a multiple bud phenotype, suggesting no apparent defect in cytokinesis (Figures S5E and S5F), although it remains possible that a weak defect in cell separation in the mutant cells was abolished by sonication. Both mutants exhibited the stereotypical phenotypes for cells with defective cell walls, including broad-neck morphology, sensitivity to CW, enhanced chitin deposition into the cell wall, and lethality suppression by the osmotic support of 1 M sorbitol (Figures S5E and S5G-S5I) (Kubo, et al, 2019;Levin, 2011;Ram and Klis, 2006). These data demonstrate that, in contrast to its fission yeast ortholog Sbg1, Kre6 is not involved in the AMR and PS-mediated membrane closure, and that its primary function is likely to be in the cell wall biogenesis or maturation.…”

Section: Genetic Perturbation Coupled With Kinetic Analysis Defines the Dual Role Of Cyk3 In The Activation Of Ps Formation And Inhibitiomentioning

confidence: 99%

The kinetic landscape and interplay of protein networks in cytokinesis

et al. 2021

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Summary Cytokinesis is executed by protein networks organized into functional modules. Individual proteins within each module have been characterized to various degrees. However, the collective behavior and interplay of the modules remain poorly understood. In this study, we conducted quantitative time-lapse imaging to analyze the accumulation kinetics of more than 20 proteins from different modules of cytokinesis in budding yeast. This analysis has led to a comprehensive picture of the kinetic landscape of cytokinesis, from actomyosin ring (AMR) assembly to cell separation. It revealed that the AMR undergoes biphasic constriction and that the switch between the constriction phases is likely triggered by AMR maturation and primary septum formation. This analysis also provided further insights into the functions of actin filaments and the transglutaminase-like protein Cyk3 in cytokinesis and, in addition, defined Kre6 as the likely enzyme that catalyzes β-1,6-glucan synthesis to drive cell wall maturation during cell growth and division.

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“…The highly pleiotropic morphological defects of ccw12 Δ cells, including the wide neck, a typical phenotype of cell wall mutants [ 45 ], and altered cell shape for both mother and daughter compartments [ 46 ], clearly indicated the important role of Ccw12 as a major structural component of the cell wall [ 47 ]. This small (133 amino acid residues) and highly glycosylated GPI-anchored protein has been previously shown to impact the maintenance of newly synthesized areas of the cell wall [ 13 ] and cell fitness [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Defining Functions of Mannoproteins in Saccharomyces cerevisiae by High-Dimensional Morphological Phenotyping

Ghanegolmohammadi

Okada

Liu

et al. 2021

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Mannoproteins are non-filamentous glycoproteins localized to the outermost layer of the yeast cell wall. The physiological roles of these structural components have not been completely elucidated due to the limited availability of appropriate tools. As the perturbation of mannoproteins may affect cell morphology, we investigated mannoprotein mutants in Saccharomyces cerevisiae via high-dimensional morphological phenotyping. The mannoprotein mutants were morphologically classified into seven groups using clustering analysis with Gaussian mixture modeling. The pleiotropic phenotypes of cluster I mutant cells (ccw12Δ) indicated that CCW12 plays major roles in cell wall organization. Cluster II (ccw14Δ, flo11Δ, srl1Δ, and tir3Δ) mutants exhibited altered mother cell size and shape. Mutants of cluster III and IV exhibited no or very small morphological defects. Cluster V (dse2Δ, egt2Δ, and sun4Δ) consisted of endoglucanase mutants with cell separation defects due to incomplete septum digestion. The cluster VI mutant cells (ecm33Δ) exhibited perturbation of apical bud growth. Cluster VII mutant cells (sag1Δ) exhibited differences in cell size and actin organization. Biochemical assays further confirmed the observed morphological defects. Further investigations based on various omics data indicated that morphological phenotyping is a complementary tool that can help with gaining a deeper understanding of the functions of mannoproteins.

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Implications of maintenance of mother–bud neck size in diverse vital processes of Saccharomyces cerevisiae

Cited by 7 publications

References 63 publications

Cell size sets the diameter of the budding yeast contractile ring

Cell size sets the diameter of the budding yeast contractile ring

The kinetic landscape and interplay of protein networks in cytokinesis

Defining Functions of Mannoproteins in Saccharomyces cerevisiae by High-Dimensional Morphological Phenotyping

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