Cell size sets the diameter of the budding yeast contractile ring

Kukhtevich, I. V.; Lohrberg, N.; Padovani, Francesco; Schneider, Robert; Schmoller, Kurt M.

doi:10.1038/s41467-020-16764-x

Cited by 32 publications

(45 citation statements)

References 49 publications

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“…In order to increase the range of observable cell volumes, we used strains with β-estradiol inducible WHI5, similarly described in previous works 18,38 . For this purpose, we deleted the endogenous alleles of the G1/S inhibitor WHI5 and integrated one copy of WHI5 expressed from an artificial, βestradiol-inducible promoter system 19 .…”

Section: Inducible-whi5 Strainmentioning

confidence: 99%

Transcription coordinates histone amounts and genome content

Claude

Bureik

Adarska

et al. 2020

Preprint

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Biochemical reactions typically depend on the concentrations of the molecules involved, and cell survival therefore critically depends on the concentration of proteins. To maintain constant protein concentrations during cell growth, global mRNA and protein synthesis rates are tightly linked to cell volume. While such regulation is appropriate for most proteins, certain cellular structures do not scale with cell volume. The most striking example of this is the genomic DNA, which doubles during the cell cycle and increases with ploidy, but is independent of cell volume.Here, we show that the amount of histone proteins is coupled to the DNA content, even though mRNA and protein synthesis globally increase with cell volume. As a consequence, and in contrast to the global trend, histone concentrations (i.e. amounts per volume) decrease with cell volume but increase with ploidy. We find that this distinct coordination of histone homeostasis and genome content is already achieved at the transcript level, and is an intrinsic property of histone promoters that does not require direct feedback mechanisms. Mathematical modelling and histone promoter truncations reveal a simple and generalizable mechanism to control the cell volume- and ploidy-dependence of a given gene through the balance of the initiation and elongation rates.

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Section: Inducible-whi5 Strainmentioning

confidence: 99%

Transcription coordinates histone amounts and genome content

Claude

Bureik

Adarska

et al. 2020

Preprint

Self Cite

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“…Further, related strategies may be used to control the growth of radial microtubule arrays that reach the cell periphery (27,28), and may explain the scaling relationships observed between flagellar length and cell length (29) and between contractile ring diameter and cell diameter (30).…”

mentioning

confidence: 99%

Scaling of subcellular actin structures with cell length through decelerated growth

McInally

Kondev

Goode

2021

eLife

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How cells tune the size of their subcellular parts to scale with cell size is a fundamental question in cell biology. Until now, most studies on the size control of organelles and other subcellular structures have focused on scaling relationships with cell volume, which can be explained by limiting pool mechanisms. Here, we uncover a distinct scaling relationship with cell length rather than volume, revealed by mathematical modeling and quantitative imaging of yeast actin cables. The extension rate of cables decelerates as they approach the rear of the cell, until cable length matches cell length. Further, the deceleration rate scales with cell length. These observations are quantitatively explained by a 'balance-point' model, which stands in contrast to the limiting pool mechanisms and that senses the linear dimensions of the cell.

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“…Similar principles may underlie the length control of other polarized, linear actin structures, such as filopodia and stereocilia. Further, related strategies may be used to control the growth of radial microtubule arrays that reach the cell periphery ( 25 , 26 ), and may explain the scaling relationships observed between flagellar length and cell length ( 27 ) and between contractile ring diameter and cell diameter ( 28 ).…”

Section: Main Textmentioning

confidence: 99%

Scaling of subcellular structures with cell length through decelerated growth

McInally

Kondev

Goode

2021

Preprint

View full text Add to dashboard Cite

How cells tune the size of their subcellular parts to scale with cell size is a fundamental question in cell biology. Until now, most studies on the size control of organelles and other subcellular structures have focused on scaling relationships with cell volume, which can be explained by limiting pool mechanisms. Here, we uncover a distinct scaling relationship with cell length rather than volume, revealed by mathematical modeling and quantitative imaging of yeast actin cables. The extension rate of cables decelerates as they approach the rear of the cell, until cable length matches cell length. Further, the deceleration rate scales with cell length. These observations reveal a new mode of scaling that senses the linear dimensions of a cell.One Sentence SummaryAs actin cables in yeast cells grow longer, their extension rate decelerates, enabling the cable length to match cell length.

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Cell size sets the diameter of the budding yeast contractile ring

Cited by 32 publications

References 49 publications

Transcription coordinates histone amounts and genome content

Transcription coordinates histone amounts and genome content

Scaling of subcellular actin structures with cell length through decelerated growth

Scaling of subcellular structures with cell length through decelerated growth

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