Mapping the genetic basis of breast microcalcifications and their role in metastasis

Rizwan, Asif; Paidi, Santosh Kumar; Zheng, Chao; Cheng, Maojie; Barman, Ishan; Glunde, Kristine

doi:10.1038/s41598-018-29330-9

Cited by 27 publications

(20 citation statements)

References 40 publications

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“…Osteopontin could be involved in the process. Silencing of osteopontin decreased the formation of hydroxyapatite in breast cancer cells in response to osteogenic cocktail . The current study demonstrated calcifications found in CNB was significantly associated with a reduced upgrade risk, suggesting the accurate sampling of targeted lesions.…”

Section: Discussionmentioning

confidence: 52%

Diagnostic upgrade of atypical ductal hyperplasia of the breast based on evaluation of histopathological features and calcification on core needle biopsy

Chen

Hu²,

Tsang

et al. 2019

Histopathology

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2019) Histopathology 75, 320-328. https://doi.org/10.1111/his.13881Diagnostic upgrade of atypical ductal hyperplasia of the breast based on evaluation of histopathological features and calcification on core needle biopsy Aims: Atypical ductal hyperplasia (ADH) of breast is increasingly diagnosed in core needle biopsy (CNB). As higher-grade lesions were found in the excision in a substantial proportion of ADH on CNB, factors predicting risk of subsequent upgrade are clinically significant. This study aims to investigate relevant histopathological factors in CNB that could predict diagnostic upgrade at excision. Methods and results: One hundred and forty-three cases of CNB with paired subsequent excision were evaluated for multiple clinicopathological parameters related to CNB sampling, ADH morphology, calcification and other co-existing histological features, and which of these parameters were associated with diagnostic upgrade at subsequent excisions were determined. Fortyeight cases (34.3%) were upgraded to malignancy, including 15 invasive cancers and 33 ductal carcinomas in situ (DCIS). An increased tissue area occupied by ADH (P = 0.026), a higher number of ADH foci (P = 0.004), the presence of solid pattern (P = 0.037) and older age (P = 0.012) were positively associated with upgrade, while negative associations were found with the presence of micropapillary pattern (P = 0.025), co-existing columnar cell lesions (CCL) (P = 0.001) and the presence of calcifications (P = 0.009). Multivariate logistic regression analysis showed that the number of ADH foci (HR = 2.810, P = 0.013) was an independent positive predictor, while co-existing CCL (HR = 0.391, P = 0.013) was an independent negative predictor for upgrade. Conclusions: Patients with ADH in CNB showing the presence of co-existing CCL and a lower number of ADH foci have a lower risk of disease upgrade at excision, and are potential candidates for observationonly management.

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Section: Discussionmentioning

confidence: 52%

Diagnostic upgrade of atypical ductal hyperplasia of the breast based on evaluation of histopathological features and calcification on core needle biopsy

Chen

Hu²,

Tsang

et al. 2019

Histopathology

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“…Among the transcriptional regulators of ADSC is SPP1, which is also secreted by adipose tissue [219]. It has been shown that SPP1 is responsible for the formation of hydroxyapatite crystals in BC cells in response to an osteogenic cocktail [220]. Furthermore, the expression of SPP1 is significantly elevated in calcified BC [221], and the expression of SPP1 also aids in bone metastasis of BC cells [222].…”

Section: Microcalcificationsmentioning

confidence: 99%

The Importance of Breast Adipose Tissue in Breast Cancer

Kothari

Diorio

Durocher

2020

IJMS

111

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Adipose tissue is a complex endocrine organ, with a role in obesity and cancer. Adipose tissue is generally linked to excessive body fat, and it is well known that the female breast is rich in adipose tissue. Hence, one can wonder: what is the role of adipose tissue in the breast and why is it required? Adipose tissue as an organ consists of adipocytes, an extracellular matrix (ECM) and immune cells, with a significant role in the dynamics of breast changes throughout the life span of a female breast from puberty, pregnancy, lactation and involution. In this review, we will discuss the importance of breast adipose tissue in breast development and its involvement in breast changes happening during pregnancy, lactation and involution. We will focus on understanding the biology of breast adipose tissue, with an overview on its involvement in the various steps of breast cancer development and progression. The interaction between the breast adipose tissue surrounding cancer cells and vice-versa modifies the tumor microenvironment in favor of cancer. Understanding this mutual interaction and the role of breast adipose tissue in the tumor microenvironment could potentially raise the possibility of overcoming breast adipose tissue mediated resistance to therapies and finding novel candidates to target breast cancer.

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“…1C) for capturing the phenotypic differences in terms of their morphological and molecular attributes. Our previous characterization of these cell lines confirmed their distinct metastatic abilities commensurate with the stage and organ from which they were isolated [31]. Our recent investigation of the biophysical properties of these cells revealed that the LM cells are most motile and least stiff, which bestow them with unique invasive capability [8].…”

Section: Resultsmentioning

confidence: 95%

Coarse Raman and optical diffraction tomographic imaging enable label-free phenotyping of isogenic breast cancer cells of varying metastatic potential

Paidi

Shah

Raj

et al. 2020

Preprint

Self Cite

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Identification of the metastatic potential represents one of the most important tasks for molecular imaging of cancer. While molecular imaging of metastases has witnessed substantial progress as an area of clinical inquiry, determining precisely what differentiates the metastatic phenotype has proven to be more elusive underscoring the need to marry emerging imaging techniques with tumor biology. In this study, we utilize both the morphological and molecular information provided by 3D optical diffraction tomography and Raman spectroscopy, respectively, to propose a label-free route for optical phenotyping of cancer cells at single-cell resolution. By using an isogenic panel of cell lines derived from MDA-MB-231 breast cancer cells that vary in their metastatic potential, we show that 3D refractive index tomograms can capture subtle morphological differences among the parental, circulating tumor cells, and lung metastatic cells. By leveraging the molecular specificity of Raman spectroscopy, we demonstrate that coarse Raman microscopy is capable of rapidly mapping a sufficient number of cells for training a random forest classifier that can accurately predict the metastatic potential of cells at a single-cell level. We also leverage multivariate curve resolution – alternating least squares decomposition of the spectral dataset to demarcate spectra from cytoplasm and nucleus, and test the feasibility of identifying metastatic phenotypes using the spectra only from the cytoplasmic and nuclear regions. Overall, our study provides a rationale for employing coarse Raman mapping to substantially reduce measurement time thereby enabling the acquisition of reasonably large training datasets that hold the key for label-free single-cell analysis and, consequently, for differentiation of indolent from aggressive phenotypes.

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Mapping the genetic basis of breast microcalcifications and their role in metastasis

Cited by 27 publications

References 40 publications

Diagnostic upgrade of atypical ductal hyperplasia of the breast based on evaluation of histopathological features and calcification on core needle biopsy

Diagnostic upgrade of atypical ductal hyperplasia of the breast based on evaluation of histopathological features and calcification on core needle biopsy

The Importance of Breast Adipose Tissue in Breast Cancer

Coarse Raman and optical diffraction tomographic imaging enable label-free phenotyping of isogenic breast cancer cells of varying metastatic potential

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