Nano-topography: Quicksand for cell cycle progression?

Giannini, Marianna; Primerano, Chiara; Berger, Liron; Giannaccini, Martina; Wang, Zhigang; Landi, Elena; Cuschieri, Alfred; Dente, Luciana; Signore, Giovanni; Raffa, Vittoria

doi:10.1016/j.nano.2018.07.002

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…33−35 Certain topographies have been shown to inhibit the formation of stress fibers. 36,37 In this study, cells cultured on the nanoporous surface exhibit longer parallel actin filaments (more evident with CHO-K1 and S273D cells) and upregulation of myosin II. Such an arrangement is consistent with the ability of the actin network to sustain contractile forces.…”

Section: ■ Discussionmentioning

confidence: 59%

“…The actin–myosin-based cytoskeleton is a dynamic system essential for contraction . Actin filament orientation and the regulation of myosin activity are influenced by the spatial organization and pattern of the substrate. − Certain topographies have been shown to inhibit the formation of stress fibers. , In this study, cells cultured on the nanoporous surface exhibit longer parallel actin filaments (more evident with CHO-K1 and S273D cells) and upregulation of myosin II. Such an arrangement is consistent with the ability of the actin network to sustain contractile forces .…”

Section: Discussionmentioning

confidence: 67%

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Nanoporosity Stimulates Cell Spreading and Focal Adhesion Formation in Cells with Mutated Paxillin

Bello

Fouillen

Badia

et al. 2020

ACS Appl. Mater. Interfaces

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We have evaluated the response to nanotopography of CHO-K1 cells that express wild-type paxillin or paxillin with mutations at serine 273 that inhibit phosphorylation. Cells were grown on nanoporous and polished titanium surfaces. With all cell types, immunofluorescence showed that adhesion and spreading were minimally affected on the treated surface and that the actin filaments were more abundant and well-aligned. Scanning electron microscopy revealed changes in cell shape and abundant filopodia with lateral nanoprotrusions in response to nanoporosity. Gene expression of proteins associated with cellular adhesion and protrusions was significantly increased on the nanoporous surface regardless of the cell type. In particular, α-actinin, Rac1, Cdc42, and ITGα1 were upregulated in S273 cells with alanine substitutions, whereas FAK, Pxn, and Src were downregulated, leading to improved focal adhesion formation. These findings suggest that the surface nanoporosity can “compensate for” the genetic mutations that affect the biomechanical relationship of cells to surfaces.

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Section: ■ Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 67%

Nanoporosity Stimulates Cell Spreading and Focal Adhesion Formation in Cells with Mutated Paxillin

Bello

Fouillen

Badia

et al. 2020

ACS Appl. Mater. Interfaces

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“…% BNNT nanocomposite films. Alternatively, it can be inferred that cell adhesion is enhanced by the electrically neutral surface of BNNTs that act as an anchoring site for cells (Giannini et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Boron Nitride Nanotube Addition Enhances the Crystallinity and Cytocompatibility of PVDF-TrFE

et al. 2019

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Analysis of the cellular response to piezoelectric materials has been driven by the discovery that many tissue components exhibit piezoelectric behavior ex vivo . In particular, polyvinylidene fluoride and the trifluoroethylene co-polymer (PVDF-TrFE) have been identified as promising piezo and ferroelectric materials with applications in energy harvesting and biosensor devices. Critically, the modulation of the structural and crystalline properties of PVDF-TrFE through annealing processes and the addition of particulate or fibrous fillers has been shown to modulate significantly the materials electromechanical properties. In this study, a PVDF-TrFE/boron-nitride nanotube composite was evaluated by modulated differential scanning calorimetry to assess the effects of boron nitride nanotube addition and thermal annealing on the composite structure and crystal behavior. An increased beta crystal formation [f(β) = 0.71] was observed following PVDF-TrFE annealing at the first crystallization temperature of 120°C. In addition, the inclusion of boron nitride nanotubes significantly increased the crystal formation behavior [f(β) = 0.76] and the mechanical properties of the material. Finally, it was observed that BNNT incorporation enhance the adherence and proliferation of human tenocyte cells in vitro .

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“…However, cell proliferation was determined by a wide variety of factors, which did not reach a unified conclusion. [71][72][73] We hypothesize that the orderly arranged nanotubes that accelerate protein adsorption enhance cellular proliferative activity. 74 The expression of surface markers or inflammatory factors in DCs represents their immune status.…”

Section: Discussionmentioning

confidence: 99%

Micro/Nanostructured Topography on Titanium Orchestrates Dendritic Cell Adhesion and Activation via β2 Integrin-FAK Signals

Yang¹,

Lin²,

Xu³

et al. 2022

IJN

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Background and Purpose In clinical application of dental implants, the functional state of dendritic cells (DCs) has been suggested to have a close relationship with the implant survival rate or speed of osseointegration. Although microscale surfaces have a stable osteogenesis property, they also incline to trigger unfavorable DCs activation and threaten the osseointegration process. Nanoscale structures have an advantage in regulating cell immune response through orchestrating cell adhesion, indicating the potential of hierarchical micro/nanostructured surface in regulation of DCs’ activation without sacrificing the advantage of microscale topography. Materials and Methods Two micro/nanostructures were fabricated based on microscale rough surfaces through anodization or alkali treatment, the sand-blasted and acid-etched (SA) surface served as control. The surface characteristics, in vitro and in vivo DC immune reactions and β2 integrin-FAK signal expression were systematically investigated. The DC responses to different surface topographies after FAK inhibition were also tested. Results Both micro/nano-modified surfaces exhibited unique composite structures, with higher hydrophilicity and lower roughness compared to the SA surface. The DCs showed relatively immature functional states with round morphologies and significantly downregulated β2 integrin-FAK levels on micro/nanostructures. Implant surfaces with micro/nano-topographies also triggered lower levels of DC inflammatory responses than SA surfaces in vivo. The inhibited FAK activation effectively reduced the differences in topography-caused DC activation and narrowed the differences in DC activation among the three groups. Conclusion Compared to the SA surface with solely micro-scale topography, titanium surfaces with hybrid micro/nano-topographies reduced DC inflammatory response by influencing their adhesion states. This regulatory effect was accompanied by the modulation of β2 integrin-FAK signal expression. The β2 integrin-FAK-mediated adhesion plays a critical role in topography-induced DC activation, which represents a potential target for material–cell interaction regulation.

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Nano-topography: Quicksand for cell cycle progression?

Cited by 4 publications

References 34 publications

Nanoporosity Stimulates Cell Spreading and Focal Adhesion Formation in Cells with Mutated Paxillin

Nanoporosity Stimulates Cell Spreading and Focal Adhesion Formation in Cells with Mutated Paxillin

Boron Nitride Nanotube Addition Enhances the Crystallinity and Cytocompatibility of PVDF-TrFE

Micro/Nanostructured Topography on Titanium Orchestrates Dendritic Cell Adhesion and Activation via β2 Integrin-FAK Signals

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