[30] Passive hemagglutination and hemolysis for estimation of antigens and antibodies

Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

“…These end-terminal sugar residues can also be bound by sugar binding proteins like lectins. When these proteins bind to the sugars on the surface of two or more erythrocytes, this causes agglutination [34]. We tested the extracts on RRBC with or without pre-treatment with trypsin.…”

Gene for an immunoglobulin-binding protein from a group G streptococcus

“…Sheep erythrocytes (Cappel Laboratories) were coated with immunoglobulin essentially as described by Adler and Adler (2). Tannic acid-treated erythrocytes were incubated in PBS containing (i) total human IgG (Sigma Chemical Co.), (ii) a human IgG3(X) myeloma protein (a generous gift from D. Segal, National Cancer Institute), or (iii) no protein and then washed with PBS.…”

Intragraft cytokine mRNA expression in rejecting and non‐rejecting vascularized xenografts

“…Blood from an anaesthetized NMRI mouse was extracted from the heart with a heparinized syringe and washed once in phosphate‐buffered saline (PBS; SVA) [15]. The blood was diluted in 4 ml PBS and centrifuged (400 g at +18°C for 35 min) over a density gradient with 4 ml Ficoll‐Paque ® (Amersham Pharmacia Biotech AB, Uppsala, Sweden).…”