Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

Jeurink, Prescilla V.; Lull, Cristina; Savelkoul, H.F.J.; Wichers, Harry J.

doi:10.1016/j.intimp.2008.04.004

Cited by 71 publications

(49 citation statements)

References 33 publications

(45 reference statements)

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“…Subsequently the dried samples were milled, mixed and sieved (particle size under 2 mm). Extraction of β-glucans was performed following previously described procedures [16]. Briefly, raw material was resuspended in deionized water and the mixture was heated to 100–110ºC with stirring for 45 minutes.…”

Section: Methodsmentioning

confidence: 99%

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Crespo

Guillén²,

Pablo-Maiso

et al. 2017

Food & Nutrition Research

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ABSTRACTβ-glucans exhibited in cell walls of several pathogens as bacteria or fungi are sensed by pathogen recognition receptors such as scavenger receptors present in antigen presenting cells, i.e., macrophages. β-glucans obtained from Shiitake mushrooms were chemically characterized. A β-glucan supplemented diet was assayed for 30 days in rabbits aiming to characterize the immune response elicited in blood-derived macrophages. M1 and M2 profiles of macrophage differentiation were confirmed in rabbits by in vitro stimulation with IFN-γ and IL-4 and marker quantification of each differentiation pathway. Blood derived macrophages from rabbits administered in vivo with the β-glucan supplemented diet showed higher IL-4, IFN-γ and RAGE together with lower IL-10 relative expression, indicative of an ongoing immune response. Differences in IL-1β, IL-13 and IL-4 expression were also found in rabbit sera by ELISA suggesting further stimulation of the adaptive response. Recent challenges in the rabbit industry include the search of diet supplements able to elicit an immune stimulation with particular interest in facing pathogens such as viruses or bacteria. β–glucans from fungi may contribute to maintain an immune steady state favouring protection and thus reducing antibiotic treatment.

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Section: Methodsmentioning

confidence: 99%

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Crespo

Guillén²,

Pablo-Maiso

et al. 2017

Food & Nutrition Research

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“…Isolation of polysaccharides and determination of total carbohydrate content Polysaccharides were isolated according to Jeurink et al (2008). Briefly, the freeze-dried water extracts were mixed with sterile distilled water at 90°C (1 g/100 mL) for 20 min and cooled to 4°C.…”

Section: Methodsmentioning

confidence: 99%

Pressurized liquids as an alternative green process to extract antiviral agents from the edible seaweed Himanthalia elongata

et al. 2010

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Pressurized liquid extraction (PLE), an environmentally friendly technique, was used to obtain antiviral compounds from the edible seaweed Himanthalia elongata. The antiviral properties of PLE extracts (hexane, ethanol, and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pre-treatment of Vero cells with 75 μg mL −1 of ethanol extract inhibited virus infection by approximately 90%, whereas the same concentration of water and hexane extracts reduced the virus infectivity to 78% and 70%, respectively. Moreover, ethanol extract was also more effective against HSV-1 intracellular replication than water and hexane extracts. The antiviral activity of water PLE extract was found to correlate with polysaccharides, since the polysaccharide-rich fraction isolated from this extract showed higher antiviral activity than the original water extract. A GC-MS characterization of the hexane and ethanol extracts showed that the antiviral activity of the hexane extract seemed to be related with the presence of fucosterol; meanwhile, in the case of the ethanol extract, other compounds, besides fucosterol, could be involved in this activity. Results demonstrated that PLE was an appropriate technique to obtain antiviral agents from H. elongata. These antiviral compounds were in addition to polysaccharides, which are the antiviral agents usually proposed when studying seaweeds.

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“…Polysaccharides were isolated according to Jeurink et al (2008). Briefly, the freezedried water extracts were mixed with sterile distilled water at 90ºC (1g 100 mL -1 ) for 20 min and cooled down to 4ºC.…”

Section: Isolation Of Polysaccharides and Determination Of Total Carbmentioning

confidence: 99%

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

et al. 2011

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Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoids sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pre-treatment of Vero cells with 75 g mL -1 of H. pluvialis ethanol extract inhibited virus infection by approx. 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50% respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour, since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication.The antiviral activity of water PLE extracts was found to correlate with polysaccharides, since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A GC-MS characterization of the H.pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid.

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Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

Cited by 71 publications

References 33 publications

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Pressurized liquids as an alternative green process to extract antiviral agents from the edible seaweed Himanthalia elongata

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

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