3’UTR mediated regulation of the cyclin D1 proto-oncogene

Cyclin D1 rare variants in UK multiple adenoma and early-onset colorectal cancer patients

“…In addition, loss of microRNA target sites as a result of 3¢UTR shortening can lead to pathogenic overexpression of the protein. 35 In our in silico analysis, however, none of the variants studied was suggested to modify a microRNA binding sequence.…”

Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles

“…34 Cells expressing a cyclin D1 that lacks a 3' UTR also show a higher rate of proliferation. 34,35 This evidence suggests the presence of negative regulatory elements in the 3' UTR of cyclin D1. It has been suggested that AU-rich elements (AREs) located within the 3' UTR are responsible for cyclin D1 destabilization, with the loss of AREs leading to an overexpression of cyclin D1 protein and a hyperproliferative phenotype.…”

“…36 Other studies have discovered that members of the microRNA-15/16 family can also target the 3' UTR of cyclin D1 mRNA, providing a form of post-transcriptional regulation. 35,[37][38][39] Importantly, these studies have shown that the deletion of the cyclin D1 3' UTR leads to the loss of both AREs and microRNA binding sites, resulting in an increase in cyclin D1 translation and overexpression. 35 Taken together, these studies suggest that the 3' UTR of cyclin D1 may be critical to cyclin D1 regulation and, as a result, may be important in regulating cellular proliferation during periods of cellular stress.…”

26S proteasome exhibits endoribonuclease activity controlled by extra-cellular stimuli

“…It has recently been shown that multiple ARE-containing mRNAs are intrinsically unstable due to their degradation by different endo-and exo-nucleases. 10,51 We wanted to investigate whether endo-RNAse activity of proteasomes also contributed to this process. Therefore, we decided to measure the level of AREcontaining mRNA in A431 cells as a function of proteasomal RNAse activity.…”