3<scp>D</scp> numerical simulation of a <scp>C</scp>oulter counter array with analysis of electrokinetic forces

Guo, Jinhong; Pui, Tze Sian; Rahman, Abdur; Kang, Yuejun

doi:10.1002/elps.201200418

Cited by 27 publications

(20 citation statements)

References 30 publications

(39 reference statements)

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“…On the other hand, microfluidic impedance cytometer provides a robust detection platform and could have great potential to address the current challenges for CTC detection due to their advantages of compactness and portability, small sampling size, ultrahigh sensitivity, and flexible choice of detection modalities [16][17].…”

Section: Introductionmentioning

confidence: 99%

Differential microfluidic sensor on printed circuit board for biological cells analysis

et al. 2015

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Coulter principal based resistive pulse sensor has been demonstrated as an important platform in biological cell detection and enumeration since several decades ago. Recently, the miniaturized micro-Coulter counter has attracted much attention due to its advantages in point of care diagnostics for on chip detection and enumeration of rare cells, such as circulating tumor cells. In this paper, we present a microfluidic cytometer with differential amplifier based on Coulter principle on a SU-8 coated printed circuit board substrate. The electrical current changes induced by the blockage of the microparticles in the sensing aperture are calibrated by polystyrene particles of standard size. Finally, HeLa cells are used to evaluate the performance of the proposed device for enumeration of biological samples. The proposed cytometer is built upon the cheap and widely available printed circuit board substrate and shows its great potential as personalized healthcare monitor.

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Section: Introductionmentioning

confidence: 99%

Differential microfluidic sensor on printed circuit board for biological cells analysis

et al. 2015

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“…2) Flow Field: The flow field in the microchannel satisfied the Navier-Stokes equation (9) and the continuity equation (10) The boundary condition at the channel wall is given by Smoluchowski formula under the assumption that the electrical double layer (EDL) is very thin (nanoscale) compared to the channel size (microscale) [18], [19] (11a)…”

Section: B Numerical Modelingmentioning

confidence: 99%

“…Based upon this assumption, the hydrodynamic force includes contributions from flows in both regions (15) is resulted from the electroosmotic flow in inner region (EDL), while is due to the flow field in outer region. and the electrophoretic force can both be expressed by the same Smoluchowski equation [18], however they have the opposite directions, which implies that these two forces are counterbalanced [19]. Finally, the net force acting on the particle is simplified as (16) where the hydrodynamic force in the outer region is calculated by integrating the fluidic stress tensor over the particle surface [11], [19] (17a)…”

Section: ) Particle Motionmentioning

confidence: 99%

Design of a Fluidic Circuit-Based Microcytometer for Circulating Tumor Cell Detection and Enumeration

Guo

Lei

et al. 2014

IEEE Trans. Biomed. Circuits Syst.

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Portable devices have been introduced to provide companion diagnostics in many applications such as personalized healthcare monitoring since several decades ago. Recently the polydimethylsiloxane (PDMS)-based microfluidic chip enables a cost effective platform for point of care diagnostics. In this paper, we present a systematic theoretical and experimental study of a novel fluidic circuit-based microcytometer. The working principle of this device is based on the characterization of the bandwidth and amplitude of the bias-voltage pulses induced by the microparticle's physical blockage of the sensing aperture. In the simulation, the amplitude and bandwidth of the bias voltage change is simply related to the microparticle translocation time and resistance change in the sensing aperture. In the modeling part, we simulate the two parameters (peak and translocation time) by considering 7 μm and 16 μm, which is used to approximately characterize the Red Blood Cells (RBCs) and Circulating Tumor Cells (CTCs). In the experimental setup, microparticles of different sizes are used to demonstrate the chip performance. Furthermore, RBCs and CTCs are detected and enumerated by the proposed chip. The microcytometry chip is presented and is expected toward the point of care clinical diagnostics.

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“…For the CFD module, the boundary condition at the channel wall is given by Smoluchowski formula assuming that the electrical double layer (EDL) is very thin compared to the channel size [13][14]; at the particle surface, the boundary condition is given by combining the particle velocity with the electroosmotic flow velocity [14]. The governing equations are as follows:…”

Section: B Flow Fieldmentioning

confidence: 99%

Electrokinetic Analysis of Cell Translocation in Low-Cost Microfluidic Cytometry for Tumor Cell Detection and Enumeration

Guo

Pui

Ban

et al. 2013

IEEE Trans. Biomed. Eng.

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Conventional Coulter counters have been introduced as an important tool in biological cell assays since several decades ago. Recently, the emerging portable Coulter counter has demonstrated its merits in point of care diagnostics, such as on chip detection and enumeration of circulating tumor cells (CTC). The working principle is based on the cell translocation time and amplitude of electrical current change that the cell induces. In this paper, we provide an analysis of a Coulter counter that evaluates the hydrodynamic and electrokinetic properties of polystyrene microparticles in a microfluidic channel. The hydrodynamic force and electrokinetic force are concurrently analyzed to determine the translocation time and the electrical current pulses induced by the particles. Finally, we characterize the chip performance for CTC detection. The experimental results validate the numerical analysis of the microfluidic chip. The presented model can provide critical insight and guidance for developing micro-Coulter counter for point of care prognosis.

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3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces

Cited by 27 publications

References 30 publications

Differential microfluidic sensor on printed circuit board for biological cells analysis

Differential microfluidic sensor on printed circuit board for biological cells analysis

Design of a Fluidic Circuit-Based Microcytometer for Circulating Tumor Cell Detection and Enumeration

Electrokinetic Analysis of Cell Translocation in Low-Cost Microfluidic Cytometry for Tumor Cell Detection and Enumeration

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