3-mercaptopropylphosphonic acid modified gold electrode for electrochemical detection of dopamine

Chen, Yu; Guo, Lirong; Chen, Wei; Yang, Xiaojing; Jin, Bo; Zheng, Li‐Min; Xia, Xing‐Hua

doi:10.1016/j.bioelechem.2008.12.005

Cited by 68 publications

(32 citation statements)

References 41 publications

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“…This showed that the immobilization procedure was effective. The XPS results were in agreement with those reported by others [25,26,27,28]. Therefore, the anti-T-Tau bonded biosensor was ready for T-Tau antigen detection.…”

Section: Methodssupporting

confidence: 91%

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV)

2017

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A single-use, in vitro biosensor for the detection of T-Tau protein in phosphate-buffer saline (PBS) and undiluted human serum was designed, manufactured, and tested. Differential pulse voltammetry (DPV) served as the transduction mechanism. This biosensor consisted of three electrodes: working, counter, and reference electrodes fabricated on a PET sheet. Both working and counter electrodes were thin gold film, 10 nm in thickness. Laser ablation technique was used to define the size and structure of the biosensor. The biosensor was produced using cost-effective roll-to-roll process. Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were employed to covalently immobilize the anti-T-Tau (T-Tau antibody) on the gold working electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) and N–hydroxysuccinimide (NHS) cross-linked anti-T-Tau to the carboxylic groups on one end of the MPA. A T-Tau protein ladder with six isoforms was used in this study. The anti-T-Tau concentration used was 500,000 pg/mL. The T-Tau protein concentration ranged from 1000 pg/mL to 100,000 pg/mL. DPV measurements showed excellent responses, with a good calibration curve. Thus, a practical tool for simple detection of T-Tau protein, a biomarker of neuro-degenerative disorders, has been successfully developed. This tool could also be extended to detect other biomarkers for neuro-degenerative disorders, such as P-Tau protein and β-amyloid 42.

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Section: Methodssupporting

confidence: 91%

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV)

2017

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“…26 The inset of Fig. 9 shows the reductive desorption of Au-Cys (solid line) and Au/Au nano -Cys (dash line) in 0.5 mol L À1 KOH.…”

Section: Oxidative and Reductive Desorption Of Cysteine Sammentioning

confidence: 99%

A novel sensor of cysteine self-assembled monolayers over gold nanoparticles for the selective determination of epinephrine in presence of sodium dodecyl sulfate

Atta

Galal

El-Ads

2012

Analyst

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A novel sensor of cysteine self-assembled monolayers over gold nanoparticles modified gold electrode has been constructed for the determination of epinephrine in presence of sodium dodecyl sulfate (Au/Au(nano)-CysSDS). Electrochemical investigation and characterization of the modified electrode are achieved using cyclic voltammetry, linear sweep voltammetry, and scanning electron microscopy. The Au/Au(nano)-CysSDS electrode current signal is remarkably stable via repeated cycles and long term stability, due to the strong Au-S bond, compared to the Au/Au(nano) electrode. The catalytic oxidation peak currents obtained from linear sweep voltammetry (LSV) increased linearly with increasing epinephrine concentrations in the range of 2 to 30 μmol L(-1) and 35 to 200 μmol L(-1) with correlation coefficients of 0.9981 and 0.9999 and a limit of detection of 0.294 nmol L(-1) and 1.49 nmol L(-1), respectively. The results showed that Au/Au(nano)-CysSDS can selectively determine epinephrine in the coexistence of a large amount of uric acid and glucose. In addition, a highly selective and simultaneous determination of tertiary mixture of ascorbic acid, epinephrine, and acetaminophen is explored at this modified electrode. Excellent recovery results were obtained for determination of epinephrine in spiked urine samples at the modified electrode. Au/Au(nano)-CysSDS can be used as a sensor with excellent reproducibility, sensitivity, and long term stability.

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“…In the previous works [15][16][17][18][19][20][21][22][23][24][25], the phosphonic acid-functionalized planar gold have been constructed by using APO 3 H 2 -terminated thiols molecules, and consequently applied in various fields such as bioelectrochemistry [15][16][17][18][19][20]26], electroanalysis [23], ZrP materials [22], biomimetic membranes [24], oriented nucleation of minerals [27]. Meanwhile, there are also a few studies dedicated to exploring the preparation and property of the phosphonic acid-functionalized Au-NPs by using APO 3 H 2 -terminated thiols molecules [6,28].…”

Section: Introductionmentioning

confidence: 98%

Facile controlled preparation of phosphonic acid-functionalized gold nanoparticles

Zhang

Zhou

Chen

et al. 2010

Journal of Colloid and Interface Science

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3-mercaptopropylphosphonic acid modified gold electrode for electrochemical detection of dopamine

Cited by 68 publications

References 41 publications

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV)

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV)

A novel sensor of cysteine self-assembled monolayers over gold nanoparticles for the selective determination of epinephrine in presence of sodium dodecyl sulfate

Facile controlled preparation of phosphonic acid-functionalized gold nanoparticles

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