3-Dimensional Optical Clearing and Imaging of Pruritic Atopic Dermatitis and Psoriasis Skin Reveals Downregulation of Epidermal Innervation

Tan, Yingrou; Ng, W.J.; Lee, Sean Zhuo Xuan; Lee, Bernett Teck Kwong; Nattkemper, Leigh; Yosipovitch, Gil; Ng, Lai Guan; Tey, Hong Liang

doi:10.1016/j.jid.2018.11.006

Cited by 42 publications

(45 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Typically, the epidermis strongly scatters and absorbs light of the visible range of the spectrum, which makes it a challenging sample for optical imaging modalities like confocal microscopy. Optical clearing, as utilized by Tan et al (2019), lowers the scattering properties of the tissue and enables volumetric imaging of the skin. Sample preparation for 3D analysis may be more difficult than for standard 2D histology, however.…”

Section: D Versus 2d: What a Third Dimension Has To Offermentioning

confidence: 99%

“…This so-called segmentation map is typically obtained by the manual assessment of experts. As pointed out by Tan et al (2019) this rather time-consuming work can be influenced by subjective decisions that are made by raters. To compensate for rater-induced errors, it is useful to combine several manual segmentations of different raters on the same image data.…”

Section: Nerve Fiber Segmentation: Manual Versus Automaticmentioning

confidence: 99%

“…To analyze large data sets quickly and to minimize subjective errors, automated segmentation algorithms have to be developed in the field of image processing science (computer vision). To find algorithms for reliable and automatic detection of fine nerve fibers is challenging, especially in images with low contrast or low signalto-noise ratios (SNR) as presented in the work by Tan et al (2019).…”

Section: Nerve Fiber Segmentation: Manual Versus Automaticmentioning

confidence: 99%

“…Recently, the Journal of Investigative Dermatology published an approach to 3-dimensionally (3D) image and analyze the innervation of skin (Tan et al (2019). This method is fundamentally different from traditional approaches that are based on 2-dimensional (2D) histology slides, as the imaging beam must pass through the superficial layers of the skin.…”

mentioning

confidence: 99%

“…Scattering properties of the stratum corneum and epidermis distort light beams in unpredictable ways such that the images typically become blurry. Tan et al (2019) used extensive tissue processing that included optical clearing techniques to overcome this obstacle. Volumetric nerve maps of skin enable the analysis of innervation architecture in normal skin and pathological situations in a way that is fundamentally different than is possible using 2D histology slides.…”

mentioning

confidence: 99%

See 4 more Smart Citations

3-Dimensional Imaging of Cutaneous Nerve Endings

Casper

2019

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Section: D Versus 2d: What a Third Dimension Has To Offermentioning

confidence: 99%

Section: Nerve Fiber Segmentation: Manual Versus Automaticmentioning

confidence: 99%

Section: Nerve Fiber Segmentation: Manual Versus Automaticmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

3-Dimensional Imaging of Cutaneous Nerve Endings

Casper

2019

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Pruritus, Prurigo and Lichen Simplex

Ständer,

Yosipovitch

2024

Rook's Textbook of Dermatology

View full text Add to dashboard Cite

Chronic pruritus is a common, complex dermatological symptom that has a profound impact on patients and society. It is highly prevelant in the majority of inflammatory skin diseases as well as in bullous autoimmune dermatoses, cutaneous lymphoma, cutaneous drug reactions and ageing skin. Chronic pruritus is also common in non‐dermatological diseases such as advanced stages of chronic kidney disease, hepatobiliary diseases and lymphoproliferative disorders, as well as neuropathies and disorders with psychogenic causes. The pathophysiology is complex and involves many itch mediators and receptors that differ between the diseases, with common neuronal pathways. The majority of types of chronic itch are transmitted by non‐histamnergic nerve fibres. In many inflammatory skin diseases a cross‐talk exists between keratinocytes, the immune system and sensory nerves. The pruritus characteristics of itch and therapy targets may differ between the different aetiological entities, but the impact on quality of life, sleep and psychosocial burden is common to all patients.

show abstract

Characteristics, mechanism, and management of pain in atopic dermatitis: A literature review

Dong

2021

Clinical & Translational All

View full text Add to dashboard Cite

Background Atopic dermatitis (AD) is a chronic, pruritic, immune‐mediated inflammatory disease. Developments in basic science and clinical research have increased our understanding of AD. Although pain as a symptom of AD is underemphasized in previous studies, multiple researchers address pain as a frequent burden of AD. However, the exact role of pain in AD is not fully understood. Aims Our review aimed to summarize the current evidence focusing on characteristics, mechanism, and management of pain in AD. Materials & Methods We conducted a thorough literature review in the PubMed database to figure out different aspects discussing pain in AD, including pain symptoms, burden, the relationship between pain and itch, mechanism, and pain management in AD. Results and Conclusion AD patients affected by skin pain vary from 42.7%‐92.2% with remarkable intensity and heavy burden. Skin pain and itch interacted both in symptoms and mechanisms. Atopic skin with the impaired barrier, neurogenic inflammation mediators, peripheral and central sensitization of pain may possibly explain pain mechanism in AD. Future research is needed to clarify the commonality and disparity of pain and itch in AD in order to seek efficacious medications and treatment.

show abstract

3-Dimensional Optical Clearing and Imaging of Pruritic Atopic Dermatitis and Psoriasis Skin Reveals Downregulation of Epidermal Innervation

Cited by 42 publications

References 13 publications

3-Dimensional Imaging of Cutaneous Nerve Endings

3-Dimensional Imaging of Cutaneous Nerve Endings

Pruritus, Prurigo and Lichen Simplex

Characteristics, mechanism, and management of pain in atopic dermatitis: A literature review

Contact Info

Product

Resources

About