3-Dehydroandrographolide protects against lipopolysaccharide-induced inflammation through the cholinergic anti-inflammatory pathway

Lu, Zibin; Xie, Pei; Zhang, Dongmei; Sun, Ping‐Hua; Yang, Huayi; Ye, Jiaxi; Cao, Hongxin; Huo, Chuying; Zhou, Huixia; Chen, Yuyao; Ye, Wen‐Cai; Yu, Linzhong; Liu, Junshan

doi:10.1016/j.bcp.2018.10.034

Cited by 34 publications

(22 citation statements)

References 63 publications

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“…Cell viability assay was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RAW 264.7 cells were seeded in a 96-well plate at a density of 8×10 4 cells/ml and cultured for 24 h. Subsequently, cell viability was analyzed as described previously (Lu et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Confocal microscopy was used to confirm the expression levels of STAT3 and P-STAT3 (Tyr705). Cells were co-incubated with LGS (200 µg/ml) and LPS (1 µg/ml) for 24 h. Western blotting and confocal microscopy were performed as described previously (Lu et al, 2018). The protein levels of Western blotting were quantified by ImageJ 1.4.3 (National Institutes of Health, USA).…”

Section: Methodsmentioning

confidence: 99%

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Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

Yang

Huo

et al. 2019

Front. Pharmacol.

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Background: Acute lung injury (ALI) is a life-threatening disease without effective chemotherapy at present. Liang-Ge-San (LGS) is a famous traditional Chinese medicine formula, which is used to treat ALI in China. However, only a few studies have addressed the mechanisms of LGS in ALI. Purpose: To evaluate the anti-inflammatory effects of LGS on lipopolysaccharide (LPS)-induced ALI, and to explore its underlying molecular mechanism. Methods: Murine RAW264.7 cells were treated with LGS and LPS (1 μg/ml). The generation of IL-6, TNF-α, IL-1β was detected by ELISA. The protein expressions of STAT3 and P-STAT3 (Tyr705) were determined by Western blotting and fluorescence confocal microscopy. STAT3 transcriptional activity was investigated by luciferase reporter gene assay. qPCR was used to detect the expressions of microRNA-21 (miR-21), STAT3, and IL-6. DSS cross-linking assay was used to assess the change of STAT3 dimer. In vivo anti-inflammatory effects of LGS were evaluated in an ALI mouse model induced by tracheal instillation of LPS (3 mg/kg). The anti-ALI effects were evaluated by ELISA, qPCR, Western blotting, BCA, and H&E assays. Results: LGS suppressed LPS-stimulated IL-6, TNF-α, and IL-1β generation in murine macrophages RAW264.7. Moreover, LGS down-regulated protein levels of P-STAT3 (Tyr705) and STAT3, inhibited STAT3 transcriptional activity, and up-regulated miR-21. Furthermore, blockage of miR-21 antagonized the inhibitory effects of LGS on the production of IL-6 and the expressions of P-STAT3 (Tyr705) and STAT3 as well as the formation of STAT3 dimer. Critically, LGS up-regulated the expression of miR-21 and inhibited the protein expressions of STAT3 and P-STAT3 (Tyr705) to reduce the release of IL-6 and inflammatory cell infiltration as well as the degree of edema in LPS-induced ALI mice. Conclusion: LGS inhibited LPS-induced ALI through up-regulating miR-21 and subsequently inhibiting the STAT3 signaling pathway, thereby decreasing the release of IL-6.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

Yang

Huo

et al. 2019

Front. Pharmacol.

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“…3-Dehydroandrographolide 61 , a natural andrographolide product, was demonstrated to decrease LPS-induced ALI in mice, associated with inactivation of the NF-κB/Akt signaling pathway. However, these protective effects were attenuated by α7nAchR siRNA or methyllycaconitine, demonstrating that 3-dehydroandrographolide protected against ALI through the cholinergic anti-inflammatory pathway [ 230 ].…”

Section: Natural Compounds That Exert Anti-ali Effectsmentioning

confidence: 99%

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Zhou

et al. 2021

Pharmacological Research

211

121

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“…RAW 264.7 cells were cultured on a glass dish at a density of 1 × 10 5 for 24 h. After being preincubated with AP-1 (10 μM) or AP-4 (10 μM) for 2 h, the following steps were carried out as previously described [41].…”

Section: Methodsmentioning

confidence: 99%

Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata

et al. 2019

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Andrographis paniculata (AP) has been widely used in China for centuries to treat various diseases, and especially to treat inflammation. Diterpenoid lactones are the main anti-inflammatory components of AP. However, systematic chemical composition and biological activities, as well as key pharmacophores, of these diterpenoid lactones from AP have not yet been clearly understood. In this study, 17 diterpenoid lactones, including 2 new compounds, were identified by spectroscopic methods, and most of them attenuated the generation of TNF-α and IL-6 in LPS-induced RAW 274.7 cells examined by ELISA. Pharmacophores of diterpenoid lactones responsible for the anti-inflammatory activities were revealed based on the quantitative structure-activity relationship (QSAR) models. Moreover, new compounds (AP-1 and AP-4) exerted anti-inflammatory activity in LPS microinjection-induced zebrafish, which might be correlated with the inhibition of the translocation of NF-κB p65 from cytoplasm to nucleus. Our study provides guidelines for future structure modification and rational drug design of diterpenoid lactones with anti-inflammatory properties in medical chemistry.

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3-Dehydroandrographolide protects against lipopolysaccharide-induced inflammation through the cholinergic anti-inflammatory pathway

Cited by 34 publications

References 63 publications

Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Diterpenoid Lactones with Anti-Inflammatory Effects from the Aerial Parts of Andrographis paniculata

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