3 D nano bilayered spatially and functionally graded scaffold impregnated bromelain conjugated magnesium doped hydroxyapatite nanoparticle for periodontal regeneration

Shoba, Ekambaram; Lakra, Rachita; Kiran, Manikantan Syamala; Korrapati, Purna Sai

doi:10.1016/j.jmbbm.2020.103822

Cited by 27 publications

(24 citation statements)

References 49 publications

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“…This effect was confirmed in our immunohistochemical analyses of the flap tissue, where we also observed an increased number of CD31 + microvessels in the medial flap zone, though the difference was not proven to be statistically significant. These findings are in line with several studies suggesting a pro-angiogenic activity of bromelain [ 26 , 27 ]. However, on the other hand, there are also studies reporting that the enzyme exerts an anti-angiogenic effect on cancer cell lines [ 18 , 28 , 29 ].…”

Section: Discussionsupporting

confidence: 92%

Bromelain Protects Critically Perfused Musculocutaneous Flap Tissue from Necrosis

et al. 2022

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Bromelain has previously been shown to prevent ischemia-induced necrosis in different types of tissues. In the present study, we, therefore, evaluated for the first time, the tissue-protective effects of bromelain in musculocutaneous flaps in mice. Adult C57BL/6N mice were randomly assigned to a bromelain treatment group and a control group. The animals were treated daily with intraperitoneal injections of 20 mg/kg bromelain or saline (control), starting 1 h before the flap elevation throughout a 10-day observation period. The random-pattern musculocutaneous flaps were raised on the backs of the animals and mounted into a dorsal skinfold chamber. Angiogenesis, nutritive blood perfusion and flap necrosis were quantitatively analyzed by means of repeated intravital fluorescence microscopy over 10 days after surgery. After the last microscopy, the flaps were harvested for additional histological and immunohistochemical analyses. Bromelain reduced necrosis of the critically perfused flap tissue by ~25%. The bromelain-treated flaps also exhibited a significantly higher functional microvessel density and an elevated formation of newly developed microvessels in the transition zone between the vital and necrotic tissues when compared to the controls. Immunohistochemical analyses demonstrated a markedly lower invasion of the myeloperoxidase-positive neutrophilic granulocytes and a significantly reduced number of cleaved caspase 3-positive apoptotic cells in the transition zone of bromelain-treated musculocutaneous flaps. These findings indicate that bromelain prevents flap necrosis by maintaining nutritive tissue perfusion and by suppressing ischemia-induced inflammation and apoptosis. Hence, bromelain may represent a promising compound to prevent ischemia-induced flap necrosis in clinical practice.

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Section: Discussionsupporting

confidence: 92%

Bromelain Protects Critically Perfused Musculocutaneous Flap Tissue from Necrosis

et al. 2022

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“…Mass-dependent hemolytic-behavior of all the tested materials was observed indicating the sensitivity of erythrocytes when in contact with the PLGA-membranes. As previously reported [ 39 , 40 , 41 , 42 , 102 ], synthetic copolymers are hemocompatible under certain circumstances, and it is very important to consider all the parameters when designing implantable constructs. In the present study, MSN-loaded membranes presented slightly higher hemocompatibility compared with the neat, unloaded membranes; however, the differences were not statistically important, probably due to the small loading percentage of hemocompatible MSNs.…”

Section: Discussionmentioning

confidence: 99%

“…For periodontal regeneration, new alveolar bone should be formed along with cementum and periodontal ligament [ 38 ]. Thus, bone healing and regeneration is ensured with engineered constructs with blood compatible surfaces, rendering hemocompatibility an important factor in materials design for tissue engineering applications [ 39 , 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospun PLGA Membranes with Incorporated Moxifloxacin-Loaded Silica-Based Mesoporous Nanocarriers for Periodontal Regeneration

et al. 2022

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Engineered electrospun membranes have emerged as promising materials in guided tissue regeneration, as they provide an appropriate framework for the formation of new functional periodontal tissues. The development of multifunctional local drug delivery systems with sustained release of drugs for prolonged infection control can be used in periodontal surgical interventions to simultaneously prohibit epithelium downgrowth and ensure proper healing and regeneration of damaged periodontal tissues. The aim of the present study was the fabrication of novel composite membranes from PLGA/moxifloxacin-loaded mesoporous nanocarriers through electrospinning and the evaluation of their drug release profiles. The addition of moxifloxacin-loaded mesoporous nanocarriers in PLGA yielded a sustained and prolonged drug release, while maintaining satisfactory mechanical strength. The freshly fabricated membranes were found to be biocompatible at masses less than 1 mg after exposure to healthy erythrocytes. Increase in the amount of polymer led to more uniform fibers with large diameters and pores. The study of the parameters of the electrospinning process indicated that increase in the applied voltage value and rotation speed of the collector led to more uniform fibers with higher diameter and larger pores, suitable for tissue regeneration applications, such as periodontal tissue regeneration.

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“…Low degradation rates are still proven to be a challenge for both PLA and PCL, thereafter, to solve this problem PLGA is used to enhance degradation rate. PLCL (for Alveolar bone regeneration), 79 PGA (for Alveolar bone, PDL and Cementum regeneration), 80 , 81 PLGA (for PDL, Alveolar bone regeneration) 39 and PVA (for PDL, Alveolar bone regeneration) 82 , 83 are also useful synthetic polymers for periodontal regeneration. Research demonstrates cellular properties for all synthetic polymers is still a big challenge.…”

Section: Synthetic Polymer-based Layered Scaffolds For Periodontal Re...mentioning

confidence: 99%

Layered scaffolds in periodontal regeneration

Abedi¹,

Rajabi²,

Kharaziha³

et al. 2022

Journal of Oral Biology and Craniofacial Research

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3 D nano bilayered spatially and functionally graded scaffold impregnated bromelain conjugated magnesium doped hydroxyapatite nanoparticle for periodontal regeneration

Cited by 27 publications

References 49 publications

Bromelain Protects Critically Perfused Musculocutaneous Flap Tissue from Necrosis

Bromelain Protects Critically Perfused Musculocutaneous Flap Tissue from Necrosis

Electrospun PLGA Membranes with Incorporated Moxifloxacin-Loaded Silica-Based Mesoporous Nanocarriers for Periodontal Regeneration

Layered scaffolds in periodontal regeneration

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