3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion.

Maddox, P.; Jenkins, David

doi:10.1136/jcp.40.10.1256

Cited by 181 publications

(68 citation statements)

References 7 publications

(2 reference statements)

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“…Again, there is good agreement between measurement and the model, showing the transition between the radiation-loss-limited and absorptive-losslimited regimes. The strong OH overtone absorption in H 2 O lowers the Q plateau to 8 ϫ 10 4 , while for D 2 O the value is higher, increasing to above 3 ϫ 10 6 .…”

Section: Figmentioning

confidence: 96%

“…Unlike their optical waveguide counterparts, wherein the photon interacts with a functionalized surface only once, 4,5 recirculation within the microcavity allows photons to interact with the surface many times. Additionally, the surface of silica-based microcavities is easily sensitized using silanization agents, 6 amines, carbohydrates, the biotin-streptavidin system, 7 or antibodies. 8 For example, silica microsphere resonators, with a properly sensitized surface, were recently used to distinguish between two strands of DNA.…”

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confidence: 99%

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Ultra-high-Q microcavity operation in H2O and D2O

Armani

Min

et al. 2005

Applied Physics Letters

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Optical microcavities provide a possible method for boosting the detection sensitivity of biomolecules. Silica-based microcavities are important because they are readily functionalized, which enables unlabeled detection. While silica resonators have been characterized in air, nearly all molecular detections are performed in solution. Therefore, it is important to determine their performance limits in an aqueous environment. In this letter, planar microtoroid resonators are used to measure the relationship between quality factor and toroid diameter at wavelengths ranging from visible to near-IR in both H 2 O and D 2 O, and results are then compared to predictions of a numerical model. Quality factors ͑Q͒ in excess of 10 8 , a factor of 100 higher than previous measurements in an aqueous environment, are observed in both High-Q and ultra-high-Q ͑UHQ͒ silica optical microcavities can perform as highly sensitive detectors; 1-3 they derive their excellent transduction abilities from long photon lifetimes within the whispering gallery of the microcavity. Unlike their optical waveguide counterparts, wherein the photon interacts with a functionalized surface only once, 4,5 recirculation within the microcavity allows photons to interact with the surface many times. Additionally, the surface of silica-based microcavities is easily sensitized using silanization agents, 6 amines, carbohydrates, the biotin-streptavidin system, 7 or antibodies. 8 For example, silica microsphere resonators, with a properly sensitized surface, were recently used to distinguish between two strands of DNA. 9 However, while detailed studies have been performed to determine the limits of a resonator's quality factor in air, 10-12 no comparable studies have been performed in water. Because most detection experiments are performed in a water-based solution, it is important to thoroughly understand the impact of this environment on the relationship between the diameter of the microtoroid resonator and the operational wavelengths of interest.To this end, UHQ silica microtoroid resonators were fabricated over a wide range ͑50-250 m͒ of major toroid diameters as shown in Fig. 1. 13 Experiments were performed in both water ͑H 2 O͒ and deuterium oxide ͑D 2 O͒. The D 2 O was purchased from Aldrich. D 2 O was chosen as the second liquid because it has the same refractive index and, in turn, the same radiation loss as H 2 O. However, its absorption at all wavelengths tested is significantly less. 14 This allowed for selective probing of the absorption-loss mechanism and verification of the model developed to describe this system.The model used finite element analysis to predict the Q-factor of microtoroid resonators immersed in water or D 2 S and accounted for two loss mechanisms: radiation loss and absorption loss. The absorption loss was numerically calculated at ͑680, 1300, 1570͒ nm using published values of H 2 O ͑0.0045, 1.12, 8.79͒ cm −1 and D 2 O ͑2.46 ϫ 10 −4 , 0.105, 0.327͒ cm −1 . 14 The modeling of radiation loss uses a fully vectorial two-dimensio...

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Section: Figmentioning

confidence: 96%

mentioning

confidence: 99%

Ultra-high-Q microcavity operation in H2O and D2O

Armani

Min

et al. 2005

Applied Physics Letters

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“…Each cornea was then bisected and placed in OCT medium (Miles Elkhart, IN, USA) and stored at À701C before 8 mm sections were cut with a cryostat. These were mounted on 3-aminopropyltriethoxysilanecoated glass slides, 24 dried for 10 min, and placed in 4% paraformaldehyde for 30 min before washing in phosphate-buffered saline solution. Sections were permeabilised with 0.1% triton X-100 (Sigma) for 10 min, washed, and placed in 5% 4-chloronaphthol (Sigma) for 20 min.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Matrix metalloproteinase distribution during early corneal wound healing

Mulholland¹,

Tuft²,

Khaw³

2004

Eye

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Aim To compare matrix metalloproteinase (MMP) localisation in anterior keratectomy (AK) and lamellar keratectomy (LK) wounds. Methods Wounds were produced in one eye of 24 rabbits. The AK wounds were made to approximately 120 mm in depth and then allowed to re-epithelialise. The LK wounds were of similar depth, but the anterior stroma and epithelium were replaced after a second deeper keratectomy had been performed. Immunohistochemistry was used to localise the MMP-1, -2, -3, and -9 at intervals from 4 h to 14 days following surgery. The contralateral eyes acted as controls.Results After an AK wound MMP-1 was present at the leading edge of migrating epithelium after 18 h, while MMP-2 and -9 were localised behind the advancing epithelial edge. The presence of these enzymes rapidly fell to low levels after epithelial closure. There was only faint MMP-3 localisation between days 3 and 7. After an LK wound, MMP-1, -3, and -9 were not detected in the stromal interface, but MMP-2 was present at all time points. Conclusions This study suggests that after an AK wound, MMP-1 is a key mediator of epithelial migration, while MMP-2 and -9, and to a lesser extent MMP-3, may participate in the remodelling of corneal stroma and the reformation of epithelial basement membrane. In contrast, an LK wound results in a much lower stimulus for MMP activation. The action of MMP-2 in stromal repair is thus partly independent of epithelial injury.

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“…For analysis of transfected fibre number and damage, 10 m cryostat sections were cut at a minimum of 10 evenly spaced levels throughout each muscle and lifted on to APES-coated slides. 51 Slides were stored at −70°C after air-drying.…”

Section: Collection Of Samplesmentioning

confidence: 99%