Matrix metalloproteinase distribution during early corneal wound healing

Mulholland, B. J.; Tuft, Stephen; Khaw, PT

doi:10.1038/sj.eye.6701557

Cited by 87 publications

(95 citation statements)

References 32 publications

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“…Fifty nine sections excised 4mm from the limbus, that were not fragmented or crushed were selected because trauma can release MMPs into the extracellular space, 13 which could be mistaken to cause pterygium remodeling. 5,7 Seven control sections were obtained from the nasal corneo-conjunctiva of patients that were undergoing evisceration for irreparably injured eyes.…”

Section: Methodsmentioning

confidence: 99%

Chronic inflammatory cells and damaged limbal cells in pterygium

Anguria

Carmichael²,

Ntuli³

et al. 2013

Afr H. Sci.

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Background: Chronic inflammation in pterygium occurrence has not been explained. Whether damaged limbal basal epithelial cells are associated with pterygium occurrence in black Africans is not clear. Objective: To explain chronic inflammation in pterygium, and to clarify whether damaged limbal basal epithelial cells were associated with pterygium occurrence in black Africans. Methods: Chronic inflammatory changes and damaged limbal basal epithelial cells were assessed in 59 samples. Results: Chronic inflammatory cells were present in 59 pterygia. Inflammatory cell count in 5 (27.8%) of 18 small pterygia was >200 (high) while in 22 (53.7%) of 41 large growths was <200 (low); p = 0.25. The proportion of pterygia with high counts tended to increase with pterygium extent. Twenty (33.9%) of 59 pterygia recurred after surgery. Ten (50%) of 20 samples had high cell counts and 10 (50%), low counts; p = 0.40. P53 expression was detected in 11 (18.6%) of 59 pterygium samples and 5 (71.4%) of 7 controls; p = 0.007. MMP 1 staining was present in 14 (23.7%) of 59 sections and 5 (71.4%) of 7 controls; p = 0.02. MMP2 in 16 (27.1%) cases and 5 (71.4%) controls; p = 0.03. MMP3 was overexpressed in 16 (27.1%) of 59 cases and 5 (71.4%) controls; p = 0.03. Conclusions: Mild chronic inflammation has a tendency to be more frequent than severe inflammation in pterygia. It is clear that damaged limbal basal epithelial cells are unlikely to be related to pterygium occurrence.

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Section: Methodsmentioning

confidence: 99%

Chronic inflammatory cells and damaged limbal cells in pterygium

Anguria

Carmichael²,

Ntuli³

et al. 2013

Afr H. Sci.

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“…Peroxynitrite and NO reduce the levels of the tissue inhibitor of metalloproteinase-1 (TIMP-1), increasing the gelatinolytic activity of MMPs in corneal fibroblasts cultivated in vitro (11) . MMP-9 is produced by epithelial cells and neutrophils and has a role in remodeling the corneal stroma after keratectomy (12) . MMP-9 is involved in corneal stroma degradation, facilitating the migration and proliferation of neo-vessels in ulcers (12) .…”

Section: Inos Expression In Corneal Lesionsmentioning

confidence: 99%

“…MMP-9 is produced by epithelial cells and neutrophils and has a role in remodeling the corneal stroma after keratectomy (12) . MMP-9 is involved in corneal stroma degradation, facilitating the migration and proliferation of neo-vessels in ulcers (12) . iNOS expression has been studied in corneal ulcers caused by alkali (10) ; however, the effect of NSAIDs on its activity is unknown.…”

Section: Inos Expression In Corneal Lesionsmentioning

confidence: 99%

Ketorolac eye drops reduce inflammation and delay re-epithelization in response to corneal alkali burn in rabbits, without affecting iNOS or MMP-9

Lima¹,

Ribeiro²,

Conceição³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Purposes: To assess the effects of 0.5% ketorolac tromethamine without preservatives on the expression of iNOS and MMP-9 in alkali burn ulcers. Methods: Twelve eyes of 120-day-old male rabbits were treated (TG) every 6 h with 0.5% ketorolac tromethamine and 12 other eyes were treated with saline solution (CG), immediately after the occurrence of ulcers by 1 M sodium hydroxide (NaOH). Re-epithelialization was monitored using fluorescein every 6 h. After 24 h, six corneas (n=6) of each group were collected (M1). The others (n=6) were collected after reepithelialization (M2). At both moments, the inflammatory infiltrate and the conditions of the newly formed epithelium were histologically analyzed. iNOS and MMP-9 were evaluated by immunohistochemistry. Results: Mean epithelialization time in TG was 55 ± 0.84 h. In CG, it was 44 ± 1.06 h (p=0.001). At M1, corneas of TG had lower inflammatory exudation compared with (p <0.001). At M2, TG revealed discrete inflammatory exudation (p>0.05) and lower numbers of epithelial layers compared with CG. The mean iNOS in stromal cells did not differ in TG over both moments compared with CG (p>0.05) At M2, the central corneal region expressed more iNOS in both groups compared with the peripheral region. No significant differences were observed in iNOS scores of epithelial immunostaining between the groups and across M1 and M2 (p=0.69). Epithelial immunostaining scores for MMP-9 did not differ in TG compared with CG (p=0.69). The average immunostaining score of MMP-9 in stromal cells showed no differences between groups or moments. There was no correlation between immunostaining of iNOS and MMP-9 or between the amount of inflammatory cells and immunostaining of iNOS. Conclusions: Use of 0.5% keratolac tromethamine reduced inflammation and delayed reepithelialization in a cornea alkali burn model without impacting the expression of iNOS or MMP-9.Keywords: Corneal ulcer/chemically induced; Ketrolac tromethamine/administration; iNOS, MMP-9 RESUMOObjetivos: Avaliarem-se os efeitos do cetorolaco de trometamina 0,5%, sem conservante, sobre a expressão da iNOS e da MMP-9, em córneas com úlceras químicas. Métodos: Doze olhos de coelhos machos, 120 dias de idade, foram tratados (GT), a cada 6 horas, com o cetorolaco de trometamina 0,5% e outros 12 com solução salina (GC), imediatamente à ocorrência de úlceras por hidróxido de sódio (NaOH) 1 mol/L. A reepitelização foi monitorada por fluresceína a cada seis horas. Decorridas 24 horas, seis córneas (n=6) de cada grupo foram colhidas (primeiro momento). As demais (n=6) o foram após a sua reepitelização (segundo momento). Em ambos os momentos, avaliaram-se o infiltrado inflamatório e as condições do epitélio neoformado (HE). Por imuno-histoquímica, avaliou-se a imunomarcação de iNOS e de MMP-9. Resultados: A média do tempo de epitelização no GT foi de 55 ± 0,84 horas. No GC, ela foi de 44 ± 1,06 horas (p=0,001). Às 24 horas, as córneas do GT apresentaram menor exsudação inflamatória (p<0,01). No segundo momento, o GT mostrou discreta ...

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“…While systemic factors are -Age, malnutrition, vitamin deficiency, zinc deficiency, trauma, hypovolaemia, hypoxia, anaemia, uremia, malignant disease, jaundice, corticosteroids, cytotoxic and antimetabolite drugs. [2][3][4][5][6][7][8] The present study has been carried out to evaluate role of local instillation of 1% feracrylum and haemocoagulase on wound healing.…”

Section: Introductionmentioning

confidence: 99%

Role of local instillation of one percent feracrylum and haemocogulase on wound healing

Singh¹,

Dhruv²,

Patle³

et al. 2016

Int J Res Med Sci

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Background: The introduction of increasingly complex and major surgical procedures during the last 50 years has inevitably been accompanied by greater problems in wound healing. Fortunately, modern surgery is relatively safe, certainly by comparison with situation 50 years ago, but it cannot be said that it is totally devoid of risks or complications, and problems of wound healing are encountered in every surgical specialty. Indeed, in various ways these complication account for a large part of morbidity and mortality of surgical treatment.The present study has been carried out to evaluate role of local instillation of 1% feracrylum and haemocoagulase on wound healing. Methods: The present study was carried out in 100 patients in the Department of General Surgery, S.S. Medical College and associated G.M. Hospital and Sanjay Gandhi Memorial Hospital, Rewa (M.P.), India. The material for the study comprised of randomly selected 100 patients. A thorough clinical examination was done to assess general condition of patients and systemic disorder. Local examination was done to make a surgical diagnosis and associated problem. In case of surgical emergency, on arrival of patient clinical examination was immediately started and history asked during resuscitation of the patient. All relevant procedures were performed before surgery. Appropriate surgical procedures were performed. Suitable local haemostatic was applied. The wound inspected on 3rd, 7th and 14th day of presence of swelling, tenderness, colour change, discharge, scar tissue and changes were recorded. Results: Most of the patients were between 21-30 years and 50 and above. The youngest being 3 years and the eldest being 75 years old. Majority of patients were operated for Perforation Peritonitis (28%) and Hernia (26%). Next common group was Intestinal Obstruction (9%) and Cholecystectomy (7%). Out of 100 cases operated 60 were as planned and rests were as emergency cases. 1% Feracrylum and Haemocoagulase was used in equal number of cases. In 24 cases 1% Feracrylum was instilled subcutaneously in 10 cases instilled at intermuscular level and in 16 cases instilled at both level. In 23 cases Haemocogulase was instilled subcutaneously in 14 cases instilled at intermuscular level and in 13 cases instilled at both level. All cases Skin Wound was closed in Interrupted fashion. Conclusions: Good surgical technique plays key role in post-operative wound healing. The essential of good surgical technique include gentle handling of tissues, meticulous haemostasis, and prevention of dead space in the wound and avoidance of tissue necrosis resulting from excessive use of diathermy or strangulation of tissues by Ligatures. 1% Feracrylum and Haemocoagulase are helpful in decreasing local infection rate by decreasing post-operative capillary oozing.

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Matrix metalloproteinase distribution during early corneal wound healing

Cited by 87 publications

References 32 publications

Chronic inflammatory cells and damaged limbal cells in pterygium

Chronic inflammatory cells and damaged limbal cells in pterygium

Ketorolac eye drops reduce inflammation and delay re-epithelization in response to corneal alkali burn in rabbits, without affecting iNOS or MMP-9

Role of local instillation of one percent feracrylum and haemocogulase on wound healing

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