2D Magnetic Sensor Array for Real-time Cell Tracking and Multi-site Detection with Increased Robustness and Flow-rate

Tang, Hao; Venkatesh, Suresh; Lin, Zhongtian; Lu, Xuyang; Saeidi, Hooman; Rather, Gulam Mohmad; Bertino, Joseph R.; Lin, Chen‐Yong; Javanmard, Mehdi; Sengupta, Kaushik

doi:10.1109/cicc.2019.8780363

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…To enable high throughput detection in this relatively small channel (120 µm) compared with other MFCs (Helou et al, 2013;Murali et al, 2016;Reisbeck et al, 2018Reisbeck et al, , p. 22, 2016Tang et al, 2019), the seal between the sensor and the microfluidic channel needed to be improved to increase the flow rate and subsequently pressure. We achieved good sealing by applying UV-ozone treatment prior to bonding the sensor chip with the PDMS, post-curing to improve the contact, and springclamping to mechanically intensify the sealing while maintaining the sensor reusability, as shown in Supplemental Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To address cancerous cell detection for NCD control, a magnetic flow cytometer (MFC) provides an alternative platform for the multi-parametric quantification of cellular information while molding PoC-friendly settings like rapid turnaround time and miniaturization without the loss of sensitivity (Helou et al, 2013;Issadore et al, 2012;Lin et al, 2016;Loureiro et al, 2011;Murali et al, 2016;Reisbeck et al, 2018Reisbeck et al, , 2016Tang et al, 2019Tang et al, , 2019Zhou et al, 2017). Magnetic biosensors replace the fluorescent (or colorimetric) label with a magnetic tag.…”

Section: Introductionmentioning

confidence: 99%

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An aptamer-based magnetic flow cytometer using matched filtering

Huang

Ray

Chan

et al. 2020

Preprint

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Facing unprecedented population-ageing, the management of noncommunicable diseases (NCDs) urgently needs a point-of-care (PoC) testing infrastructure. Magnetic flow cytometers are one such solution for rapid cancer cellular detection in a PoC setting. In this work, we report a giant magnetoresistive spin-valve (GMR SV) biosensor array with a multi-stripe sensor geometry and matched filtering to improve detection accuracy without compromising throughput. The carefully designed sensor geometry generates a characteristic signature when cells labeled with magnetic nanoparticles (MNPs) pass by thus enabling multi-parametric measurement like optical flow cytometers (FCMs). Enumeration and multi-parametric information were successfully measured across two decades of throughput. 10-µm polymer microspheres were used as a biomimetic model where MNPs and MNP-decorated polymer conjugates were flown over the GMR SV sensor array and detected with a signal-to-noise ratio (SNR) as low as 2.5 dB due to the processing gain afforded by the matched filtering. The performance was compared against optical observation, exhibiting a 92% detection efficiency. The system achieved a 95% counting accuracy for biomimetic models and 98% for aptamer-based pancreatic cancer cell detection. This system demonstrates the ability to perform reliable PoC diagnostics towards the benefit for NCD control plans.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An aptamer-based magnetic flow cytometer using matched filtering

Huang

Ray

Chan

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…3B) with staggered placement to ensure detection without flow focusing, permitting an overall flow rate above 2 mL h −1 . 69 In this design, the sensing element is a spiral inductor implemented with the top-most metal layer of the CMOS process. Passing MNP-labeled cells with non-unit susceptibility effectively behave as a “core” for the inductor, slightly shifting its inductance.…”

Section: High-performance Assays Using Cmos-integrated Microfluidic C...mentioning

confidence: 99%

The next generation of hybrid microfluidic/integrated circuit chips: recent and upcoming advances in high-speed, high-throughput, and multifunctional lab-on-IC systems

2023

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Highly stable integration of graphene Hall sensors on a microfluidic platform for magnetic sensing in whole blood

et al. 2023

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The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics. Micro-Hall detectors (μHD) for magnetic detection in blood have previously demonstrated ultrahigh sensitivity to rare cells. This sensitivity originates from the minimal magnetic background in blood, obviating cumbersome and detrimental sample preparation. However, the translation of this technology to clinical applications has been limited by inherently low throughput (<1 mL/h), susceptibility to clogging, and incompatibility with commercial CMOS foundry processing. To help overcome these challenges, we have developed CMOS-compatible graphene Hall sensors for integration with PDMS microfluidics for magnetic sensing in blood. We demonstrate that these graphene μHDs can match the performance of the best published μHDs, can be passivated for robust use with whole blood, and can be integrated with microfluidics and sensing electronics for in-flow detection of magnetic beads. We show a proof-of-concept validation of our system on a silicon substrate and detect magnetic agarose beads, as a model for cells, demonstrating promise for future integration in clinical applications with a custom CMOS chip.

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2D Magnetic Sensor Array for Real-time Cell Tracking and Multi-site Detection with Increased Robustness and Flow-rate

Cited by 8 publications

References 6 publications

An aptamer-based magnetic flow cytometer using matched filtering

An aptamer-based magnetic flow cytometer using matched filtering

The next generation of hybrid microfluidic/integrated circuit chips: recent and upcoming advances in high-speed, high-throughput, and multifunctional lab-on-IC systems

Highly stable integration of graphene Hall sensors on a microfluidic platform for magnetic sensing in whole blood

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