2D Magnetic Sensor Array for Real-time Cell Tracking and Multi-site Detection with Increased Robustness and Flow-rate

Tang, Hao; Venkatesh, Suresh; Lin, Zhongtian; Lu, Xuyang; Saeidi, Hooman; Rather, Gulam M.; Bertino, Joseph R.; Lin, Chen-Yong; Javanmard, Mehdi; Sengupta, Kaushik

doi:10.1109/cicc.2019.8780363

Cited by 6 publications

References 6 publications

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“…To enable high throughput detection in this relatively small channel (120 µm) compared with other MFCs (Helou et al, 2013;Murali et al, 2016;Reisbeck et al, 2018Reisbeck et al, , p. 22, 2016Tang et al, 2019), the seal between the sensor and the microfluidic channel needed to be improved to increase the flow rate and subsequently pressure. We achieved good sealing by applying UV-ozone treatment prior to bonding the sensor chip with the PDMS, post-curing to improve the contact, and springclamping to mechanically intensify the sealing while maintaining the sensor reusability, as shown in Supplemental Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To address cancerous cell detection for NCD control, a magnetic flow cytometer (MFC) provides an alternative platform for the multi-parametric quantification of cellular information while molding PoC-friendly settings like rapid turnaround time and miniaturization without the loss of sensitivity (Helou et al, 2013;Issadore et al, 2012;Lin et al, 2016;Loureiro et al, 2011;Murali et al, 2016;Reisbeck et al, 2018Reisbeck et al, , 2016Tang et al, 2019Tang et al, , 2019Zhou et al, 2017). Magnetic biosensors replace the fluorescent (or colorimetric) label with a magnetic tag.…”

Section: Introductionmentioning

confidence: 99%

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An aptamer-based magnetic flow cytometer using matched filtering

Huang

Ray

Chan

et al. 2020

Preprint

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Facing unprecedented population-ageing, the management of noncommunicable diseases (NCDs) urgently needs a point-of-care (PoC) testing infrastructure. Magnetic flow cytometers are one such solution for rapid cancer cellular detection in a PoC setting. In this work, we report a giant magnetoresistive spin-valve (GMR SV) biosensor array with a multi-stripe sensor geometry and matched filtering to improve detection accuracy without compromising throughput. The carefully designed sensor geometry generates a characteristic signature when cells labeled with magnetic nanoparticles (MNPs) pass by thus enabling multi-parametric measurement like optical flow cytometers (FCMs). Enumeration and multi-parametric information were successfully measured across two decades of throughput. 10-µm polymer microspheres were used as a biomimetic model where MNPs and MNP-decorated polymer conjugates were flown over the GMR SV sensor array and detected with a signal-to-noise ratio (SNR) as low as 2.5 dB due to the processing gain afforded by the matched filtering. The performance was compared against optical observation, exhibiting a 92% detection efficiency. The system achieved a 95% counting accuracy for biomimetic models and 98% for aptamer-based pancreatic cancer cell detection. This system demonstrates the ability to perform reliable PoC diagnostics towards the benefit for NCD control plans.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%