The anti-inflammatory and anti-oxidative actions of eugenol improve lipopolysaccharide-induced lung injury

Magalhães, Clarissa Bichara; Casquilho, Nathalia V.; Machado, Mariana; Riva, Douglas; Travassos, Leonardo H.; Leal-Cardoso, José Henrique; Fortunato, Rodrigo S.; Faffe, Débora S.; Zin, Walter A.

doi:10.1016/j.resp.2018.07.001

Cited by 45 publications

(26 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this regard, eugenol was proven to mitigate leukocyte rolling, adhesion, and migration to the inflammatory site [86]. These results are supported by other studies performed on LPS-treated mice in which eugenol reduced lung infiltration with neutrophils/macrophages [87] and mitigated the release of inflammatory cytokines (TNF-α, IL-1β, and IL-6) [88] and the activation of NF-κB [87]. Moreover, in a murine model of ovalbumininduced allergic asthma, eugenol inhibited eosinophil lung tissue infiltration and reduced the levels of both ovalbuminspecific IgE as well as IL-4 and IL-5, the key cytokines in allergic pathologies, thereby suppressing the generation of a Th2-type immune response [89].…”

Section: Anti-inflammatory and Antioxidant Activities Ofsupporting

confidence: 64%

Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown

Aburel

Pavel

Dănilă

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Phytocompounds and medicinal herbs were used in traditional ancient medicine and are nowadays increasingly screened in both experimental and clinical settings due to their beneficial effects in several major pathologies. Similar to the drug industry, phytotherapy is interested in using nanobased delivery systems to view the identification and characterization of the cellular and molecular therapeutic targets of plant components. Eugenol, the major phenolic constituent of clove essential oil, is a particularly versatile phytochemical with a vast range of therapeutic properties, among which the anti-inflammatory, antioxidant, and anticarcinogenic effects have been systematically addressed. In the past decade, with the emerging understanding of the role of mitochondria as critical organelles in the pathophysiology of noncommunicable diseases, research regarding the role of phytochemicals as modulators of bioenergetics and metabolism is on a rise. Here, we present a brief overview of the major pharmacological properties of eugenol, with special emphasis on its applications in dental medicine, and provide preliminary data regarding its effects, alone, and included in polyurethane nanostructures, on mitochondrial bioenergetics, and glycolysis in human HaCaT keratinocytes.

show abstract

Section: Anti-inflammatory and Antioxidant Activities Ofsupporting

confidence: 64%

Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown

Aburel

Pavel

Dănilă

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…During the process of pulmonary diseases, inflammatory reaction plays an important role (Welbourn and Young, 1992; Ghio and Devlin, 2001; Ricard et al, 2001; Peng et al, 2004; Menezes et al, 2005). Many preliminary studies confirmed the anti-inflammatory and immune-regulatory effects of natural products, while several data showed a decreased effect of their metabolites on cytokines for inflammation, such as IL-1, IL-6, and TNF-α (Teng et al, 2016, Teng et al, 2017; Chen et al, 2018, Chen et al, 2019; Gupta et al, 2019; Magalhães et al, 2019; Saudagar and Saokar, 2019; Su et al, 2019; Zhang et al, 2019). Hence, it arouses our interest that whether MXD, a famous traditional Chinese medicine formula widely used in respiratory diseases, can alleviate PM2.5 induced lung injury.…”

Section: Discussionmentioning

confidence: 93%

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

et al. 2019

View full text Add to dashboard Cite

Ma Xing Shi Gan Decoction (MXD), a classical traditional Chinese medicine prescription, is widely used for the treatment of upper respiratory tract infection. However, the effect of MXD against particulate matters with diameter of less than 2.5 μm (PM2.5) induced lung injury remains to be elucidated. In this study, rats were stimulated with PM2.5 to induce lung injury. MXD was given orally once daily for five days. Lung tissues were harvested to assess pathological changes and edema. Myeloperoxidase (MPO) activity and malonaldehyde (MDA) content in lung were determined to evaluate the degree of injury. To assess the barrier disruption, the bronchoalveolar lavage fluid (BALF) was collected to determine the total protein content and count the number of neutrophils and macrophages. For evaluating the activation of macrophage in lung tissue, CD68 was detected using immunohistochemistry (IHC). The levels of inflammatory factors including tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and interleukin-6 (IL-6) in BALF and serum were measured. In vitro, a PM2.5-activated RAW 264.7 macrophages inflammatory model was introduced. To evaluate the protective effect of MXD-medicated serum, the cell viability and the release of inflammatory factors were measured. The effects of MXD on the High mobility group box-1/Toll-like receptor 4/Nuclear factor-kappa B (HMGB1/TLR4/NFκB) pathway in lung tissue and RAW 264.7 cells were assessed by Western blot. For further confirming the protective effect of MXD was mediated by inhibiting the HMGB1/TLR4/NFκB pathway, RAW 264.7 cells were incubated with MXD-medicated serum alone or MXD-medicated serum plus recombinant HMGB1 (rHMGB1). MXD significantly ameliorated the lung injury in rats, as evidenced by decreases in the pathological score, lung edema, MPO activity, MDA content, CD68 positive macrophages number, disruption of alveolar capillary barrier and the levels of inflammatory factors. In vitro, MXD-medicated serum increased cell viability and inhibited the release of inflammatory cytokines. Furthermore, MXD treatment was found to inhibit HMGB1/TLR4/NFκB signal pathway both in vivo and in vitro. Moreover, the protection of MXD could be reversed by rHMGB1 in RAW 264.7. Taken together, these results suggest MXD protects rats from PM2.5 induced acute lung injury, possibly through the modulation of HMGB1/TLR4/NFκB pathway and inflammatory responses.

show abstract

“…They were fed with standard laboratory food and water ad libitium . Eugenol and cisplatin doses were selected from doses used by Kilic et al (), Magalhaes et al (), Sakat et al (). In our study, 24 male albino Sprague Dawley albino rats were divided into four main groups ( n = 6): In the control group, no treatment was applied. Single dose of cisplatin (15 mg/kg b.wt) was administered intraperitoneally to cisplatin group. Eugenol (100 mg/kg b.wt./day) was given to eugenol group intraperitoneally daily for 5 days. Eugenol (100 mg/kg i.p.…”

Section: Methodsmentioning

confidence: 99%

The antiapoptotic and antioxidant effects of eugenol against cisplatin‐induced testicular damage in the experimental model

et al. 2019

View full text Add to dashboard Cite

Testicular dysfunction or damage is among the critical side effects of chemotherapeutic drugs like cisplatin. This study was mapped out to assess the possible therapeutic effect of eugenol on cisplatin‐induced testicular damage. In this experimental study, a single dose of cisplatin (15 mg/kg) was given intraperitoneally. After 72 hr of cisplatin injection, rats were sacrificed and testis tissues were removed. Tissues were examined by biochemical, histopathological and immunohistochemical methods. While tissue lipid peroxidation product and apoptotic marker levels increased, antioxidant enzyme activities of testis tissue were decreased in the cisplatin group. Additionally, histopathological damage was also determined in testis tissue. Contrary to all these results, the severity of damage in the tissue was reduced histopathologically owing to eugenol treatment. The lipid peroxidation decreased and antioxidant enzyme activities increased in the eugenol treatment group. It has been determined that eugenol has a therapeutic effect on oxidative stress and apoptosis against cisplatin‐induced testicular damage.

show abstract

The anti-inflammatory and anti-oxidative actions of eugenol improve lipopolysaccharide-induced lung injury

Cited by 45 publications

References 37 publications

Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown

Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

The antiapoptotic and antioxidant effects of eugenol against cisplatin‐induced testicular damage in the experimental model

Contact Info

Product

Resources

About