CRISPR RNA and anti-CRISPR protein binding to the Xanthomonas albilineans Csy1-Csy2 heterodimer in the type I-F CRISPR-Cas system

Abstract

There was an error in the name of a bacterial species used in Fig. 4C

Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR–Cas complex revealed by cryo-EM

“…S9). It is noteworthy, however, that the majority of interactions occur on the nucleobases of this 5′ handle, supporting a sequencespecific recognition of the crRNA 5′ handle by Cas5f and Cas8f, consistent with previous biochemical findings (20). α-helix (β 1 β 2 α 1 β 3 β 4 ), and two copies bind to a single Csy complex.…”

A Type I-F Anti-CRISPR Protein Inhibits the CRISPR-Cas Surveillance Complex by ADP-Ribosylation

“…Next, we tested whether AcrIF11 could modify the Cas8f subunit in the form of the Cas5f-Cas8f subcomplex alone, because these two subunits could form a stable complex (Hong et al, 2018). Ac-rIF11 only ADP-ribosylated the Cas8f subunit in the context of the whole Csy complex, but not the Cas5f-Cas8f subcomplex (Figure 4C).…”

Anti-CRISPR Phages Cooperate to Overcome CRISPR-Cas Immunity

“…All Acrs characterized to date function by inhibiting either the CRISPR surveillance complexes or the effector nucleases (Bondy-Denomy et al, 2015;Pawluk et al, 2016bPawluk et al, , 2017Wang et al, 2016aWang et al, , 2016bChowdhury et al, 2017;Dong et al, 2017;Guo et al, 2017;Harrington et al, 2017;Hynes et al, 2017;Peng et al, 2017;Rauch et al, 2017;Shin et al, 2017;Yang and Patel, 2017;Hong et al, 2018). These acr genes were first identified in temperate Pseudomonas phages (Bondy-Denomy et al, 2013) and can rescue phage from CRISPR-mediated extinction (van Houte et al, 2016b).…”