Neurotrophin expression and histomorphometric evaluation in Wistar rats subjected to neural mobilization after compression of the median nerve

Abstract

ObjectiveTo evaluate the neurotrophin mRNA expression and axon count in the median nerve of Wistar rats submitted to neural mobilization (NM) after nerve compression.MethodsEighteen animals were randomly divided into G1 (nerve compression only), G2 (NM for 1 min), and G3 (NM for 3 min). For NM, the animals were anesthetized and the right scapula received the mobilization, adapted as indicated for humans, on alternate days, from the third to the 13th postoperative (PO) day, totaling six days of therapy. On the 14th PO day, animals were anesthetized and euthanized. Fragments of the median nerve, distal to the compression procedure, were removed for histomorphometric analysis and expression of neurotrophins, nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF) by RT-PCR.ResultsHistomorphometric analysis revealed differences in the number of axons in the injured side, which was significantly lower in the injured limb nerve compared to the control limb, whereas the RT-PCR analysis showed no significant differences in the expression of NGF or BDNF.ConclusionNM treatment did not affect median nerve regeneration, which maintained normal recovery rates.

Atuação De Enfermeiro No Centro De Terapia Intensiva No Pósoperatório Imediato Com Complicações Anestésicas

“…El resultado de nuestra revisión no concuerda con el metaanálisis realizado por Basson que obtiene un TE mucho más alto (25), Nuestros resultados sobre pruebas de evocación contradicen la investigación realizada sobre el STC que señala que las técnicas de movilización neural producen una disminución de la sensibilidad nerviosa (26) disminuyendo la liberación de citoquinas (27) y aumentando la concentración en sangre de sustancias analgésicas. Apoyando nuestros resultados existen estudios de laboratorio que señalan que la aplicación de la movilización neural no modifica la sensación nociceptiva tras la compresión del nervio mediano (28) y que la técnica no altera el proceso de regeneración nerviosa (29). Nuestro resultado coincide con otra revisión (25) que indica la falta de efecto de esta técnica sobre el test de Phalen.…”

Validation of reference genes for RT-qPCR in cardiac tissue of rats induced to obesity and diabetes

“…Therefore, in the present research, due to obesity induction being performed with the supply of a hyperlipidic diet, the validation of the reference gene is of crucial importance in the correct interpretation of the final data. Development, v. 9, n. 11, e1599119702, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i11.9702 In a recent systematic review, 1700 papers were evaluated and found that most had inadequate standardization procedures or did not possess them (Bustin et al, 2013) and some recent studies actually do not mention validation of genes (Li et al, 2014;Silva et al, 2015;and Marcioli et al, 2018). One of the possible causes of this fact is the lack of information about the importance of the validation of the reference genes in each tissue to be studied, not only in the studied species.…”

Effects of joint and nerve mobilisation on neuroimmune responses in animals and humans with neuromusculoskeletal conditions: a systematic review and meta-analysis

“…The 13 animal studies compared nerve mobilisation, 22,25,49,[75][76][77] spinal mobilisation, 65 spinal manipulation, 24,84,85 and knee 68 and ankle 50,72 mobilisation with no intervention or sham in male Wistar rats (n 5 98), 22,24,25,49,50,68,[75][76][77] Sprague-Dawley rats (n 5 26), 84,85 Swiss mice (n 5 16), 72 and female Wistar rats (n 5 6). 65 Spinal manipulation and mobilisation was mimicked using an activator-assisted spinal device 24,84,85 or computer-controlled feedback motor.…”

“…65 Spinal manipulation and mobilisation was mimicked using an activator-assisted spinal device 24,84,85 or computer-controlled feedback motor. 65 Several models for neuropathic pain were used, such as chronic constriction injury (CCI) to the sciatic nerve, 22,25,[75][76][77] crush injury to the sciatic 50 or median 47,48,49 nerve, compression-decompression of the DRG, 85 and injection of inflammatory mediators within the intervertebral foramen. 84 Ankle joint inflammation, 68,69,83 chronic postischemia hind paw pain, 72 knee joint immobilisation, 24 and nerve growth factor (NGF)-induced back pain 65 were also used.…”

“…A wide range of outcome measures was evaluated, namely, neurotrophins (NGF, 22,49,76 brain-derived neurotrophic factor [BDNF]), 25,49 cytokines (tumor necrosis factor [TNF]-a, IL-1b, and IL-10), 85 expression of opioid receptors (d-opioid, k-opioid, and m-opioid), 75,77 whole-genome expression, 68 neuroinflammatory markers (astrocyte marker GFAP, 25,50,76 microglial markers CD11b/c, 25,50 and/or OX-42 25 ), nonneuronal cell proliferation, 84,85 substance-P, 77 TRPV1, 77 calcitonin generelated protein (CGRP), 65 oxidative stress markers (lipid hydroperoxide, 24 nitric oxide metabolites, 24 malondialdehyde, 72 and carbonyl protein 72 ), and antioxidant enzymes (catalase activity, 24,72 superoxide dismutase, 24,72 and glutathione peroxidase 24 ). Moreover, the outcomes were measured at different locations, such as in the serum, nerve, DRG, spinal cord, and brain.…”