Solvent-free preparation of porous poly(l-lactide) microcarriers for cell culture

Kuterbekov, Mirasbek; Machillot, Paul; Lhuissier, Pierre; Picart, Catherine; Jonas, Alain M.; Glinel, Karine

doi:10.1016/j.actbio.2018.06.009

Cited by 24 publications

(20 citation statements)

References 44 publications

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“…Indeed, compared to other widely-used manufacturing methods such as freeze-drying for example, the porosity can be controlled as well as the external shape of the scaffold [37]. Our previous studies had shown that the polyelectrolyte film can be deposited on PLGA cylinders [21] and PLA microcarriers [38], which was confirmed here on 3D printed scaffolds. The PLA being inert, it does not provide osteoinduction and the osteoinductive properties are exclusively provided via the BMP-2-containing film coating.…”

Section: Discussionsupporting

confidence: 69%

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Bouyer

Garot

Machillot

et al. 2020

Preprint

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Abstractthe reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed a new critical-size mandibular bone defect model on a mini-pig, close to human clinical issues. We analyzed the bone reconstruction obtained by a 3D printed scaffold made of clinical-grade PLA, coated with a polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). We compared the results (CT-scan, μCT, histology) to the gold standard solution, bone autograft. We demonstrated that the dose of BMP-2 delivered from the scaffold significantly influenced the amount of regenerated bone and the repair kinetics, with a clear BMP-2 dose-dependence. Bone was homogeneously formed inside the scaffold without ectopic bone formation. The bone repair was as good as for the bone autograft. The BMP-2 doses applied in our study were reduced 20 to 75-fold compared to the commercial collagen sponges used in the current clinical applications, without any adverse effects. 3D printed PLA scaffolds loaded with reduced doses of BMP-2 can be a safe and simple solution for large bone defects faced in the clinic.

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Section: Discussionsupporting

confidence: 69%

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Bouyer

Garot

Machillot

et al. 2020

Preprint

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“…Interestingly, AP was the fourth important variable with its absence from the medium leading to a higher ALP expression. AP is an integral part of standard OM formulations [7], mainly responsible for the deposition of the extracellular matrix. Why its presence had an inhibitory effect on the extent of ALP expression is unknown but warrants a further study.…”

Section: Discussionmentioning

confidence: 99%

“…Given the sensitivity of the cells to the culture conditions, such replacements must be studied in detail to ensure that they do not alter cellular functions. Significant advantages conferred on hASCs by the use of hPL, e.g., increased proliferative potential [5] and improved chromosomal stability [6], have already led to its use as the serum supplement, including in our previous study [7]. However, some reports have indicated that hPL might lead to the spontaneous expression of alkaline phosphatase (ALP), an important marker of the osteogenic differentiation [8].…”

Section: Introductionmentioning

confidence: 99%

Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells

et al. 2019

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Background Human adipose-derived stromal cells (hASCs) have been gaining increasing popularity in regenerative medicine thanks to their multipotency, ease of collection, and efficient culture. Similarly to other stromal cells, their function is particularly sensitive to the culture conditions, including the composition of the culture medium. Given the large number of parameters that can play a role in their specification, the rapid assessment would be beneficial to allow the optimization of their culture parameters. Method Herein we used the design of experiments (DOE) method to rapidly screen the influence and relevance of several culture parameters on the osteogenic differentiation of hASCs. Specifically, seven cell culture parameters were selected for this study based on a literature review. These parameters included the source of hASCs (the different providers having different methods for processing the cells prior to their external use), the source of serum (fetal bovine serum vs. human platelet lysate), and several soluble osteoinductive factors, including dexamethasone and a potent growth factor, the bone morphogenetic protein-9 (BMP-9). The expression of alkaline phosphatase was quantified as a readout for the osteogenic differentiation of hASCs. Results The DOE analysis enabled to classify the seven studied parameters according to their relative influence on the osteogenic differentiation of hASCs. Notably, the source of serum was found to have a major effect on the osteogenic differentiation of hASCs as well as their origin (different providers) and the presence of L-ascorbate-2-phosphate and BMP-9. Conclusion The DOE-based screening is a valuable approach for the classification of the impact of several cell culture parameters on the osteogenic differentiation of hASCs. Electronic supplementary material The online version of this article (10.1186/s13287-019-1333-7) contains supplementary material, which is available to authorized users.

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“…33 The design of MCs with micro-nano roughness or porous structure results in higher cell adhesion, differentiation, and therapeutic drug loading capacity. [33][34][35][36][37] Nonetheless, all these methods need to change the bulk properties of MCs which is costly, timeconsuming, and hard to synthesize and may not give the desired surface property required for directing stem cell fate. Therefore, developing a facile method for surface engineering of MCs with the synthetic material with high mechanical stability, biocompatibility, and proliferation yield is necessary.…”

Section: Introductionmentioning

confidence: 99%

Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells

et al. 2020

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Solvent-free preparation of porous poly(l-lactide) microcarriers for cell culture

Cited by 24 publications

References 44 publications

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells

Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells

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