With a new clip technique surgically inducing varicocele in Sprague-Dawley rats

Guo, Wanshou; Yang, Cheng; Bian, Jun; Xia, Hui; Yang, Jing; Zhou, Qianling; Chen, Ming-Kun; Xue, Kangyi; Zhang, Wansong; Wang, Peng; Li, Xin; Liu, Cundong

doi:10.1186/s12894-018-0350-7

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…The results of this study showed that the exosome derived from bone marrow mesenchymal stem cells could pass through the blood-testis barrier to the testicular tissue, and it was con rmed by cell experiments that they could be absorbed by spermatogonia. In rat testicular IRI model [28], compared with PBS treatment group and sham operation group after testicular reduction, although exosome derived from bone marrow mesenchymal stem cells could not prevent testicular ischemia-reperfusion injury, it could signi cantly improve the degree of testicular injury and protect the integrity and spermatogenic function of testicular tissue to a great extent through the detection of testicular histomorphology and related oxidative stress indexes. It provides a new idea for preventing ischemia-reperfusion injury after testicular torsion reduction.…”

Section: Discussionmentioning

confidence: 98%

“…The incision was primarily closed with a 4/0 silk suture. After 4 hours, by using the same incision line, testis was counter rotated to the natural position and reinserted into the scrotum to induce reperfusion for 2 hours [28].After that ,the two groups ,experimental and Treated group, were respectively injected from the tail vein with the same amount of saline and 100ug/ml exosomes. At the end of the reperfusion period rats were decapitated and testis tissues were taken for biochemical analyses and histological evaluations.…”

Section: Animal Experimentsmentioning

confidence: 99%

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Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Tian

Zhang

Yang

et al. 2021

Preprint

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Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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Section: Discussionmentioning

confidence: 98%

Section: Animal Experimentsmentioning

confidence: 99%

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Tian

Zhang

Yang

et al. 2021

Preprint

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“…Some studies have reported that varicocele causes a decrease in testicular weight and volume (Guo et al., 2018; Lipshultz & Corriere, 1977), while in others it does not affect (Bitkin et al., 2019; Zhang et al., 2018). In the present study, although testicular weight decreased significantly in the V group compared with the other groups, there was no significant difference between the groups in terms of testicular volume.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone improves testicular damage and sperm quality in experimentally varicocele‐induced adolescent rats

et al. 2021

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The aim of this study was to investigate the protective and therapeutic effects of thymoquinone against the negative effects of varicocele on testicular tissue and sperm morphology. Five groups were formed by random selection from a total of 40 adult male Wistar rats (n = 8). Thymoquinone (5 mg/kg/day) was administered intraperitoneally to the varicocele‐dimethyl sulfoxide‐olive oil‐thymoquinone (VT) group and the sham‐thymoquinone group. At the end of the 60th day, all groups were anaesthetised and the left testis was removed from the body quickly. One half of the testis tissue, which was divided into two, was separated for biochemical and Western blot analysis, while the other half were fixed in Bouin's fixative. As a result of biochemical, molecular and histopathological analyses, a statistically significant increase was found in the varicocele group testicular tissues in the malondialdehyde level, apoptotic index, Bax expression, cytochrome c expression and Bax/Bcl‐2 ratio compared with the sham group. In addition, histopathological changes characterised by partial or complete degeneration of the germinal epithelium were observed in the seminiferous tubules in the same group. Total oxidant status level and sperm count with abnormal morphology increased in varicocele group, whereas total antioxidant status level decreased. In the VT group, all of the biochemical, molecular and histopathological changes detected in the varicocele group were statistically significantly reduced. When the findings obtained in this study are evaluated, it can be said that thymoquinone has the potential to be used as a preventive and therapeutic pharmacological agent in the medical treatment of varicocele. Although the exact mechanism of action of thymoquinone has not been fully elucidated, the findings obtained in this study support the view that thymoquinone showed a cytoprotective effect by reducing apoptosis, oxidative stress and lipid peroxidation.

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“…Rats were housed in a climate-controlled environment with free access to food and water under a 12-h day/night cycle. Experimental rat varicocele model was constructed according to our new clip technique [13]. The establishment and assessment of the rat model is described in the previous articles published by our team.…”

Section: The Establishment and Assessment Of The Experimental Rat Varmentioning

confidence: 99%

Down regulating PHGDH affects the lactate production of sertoli cells in varicocele

Guo

Huang

Yang

et al. 2020

Reprod Biol Endocrinol

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Background: Although varicocele is considered to be one of the leading causes of male infertility, the precise mechanism underlying how varicocele leads to male infertility is not completely understood. We found the lactate concentration on the varicocele side of the patients was decreased compare with peripheral venous blood. In the testicles, the lactate produced by the sertoli cells through the glycolysis pathway provides most of the energy needed for spermatogenesis, the reduction of lactate will affect spermatogenesis. The objective of this study was to investigate the mechanism of this abnormal energy metabolism phenomenon in varicocele. Methods: In this study, we collected the testicular tissue from patients with varicocele, the glycolysis related proteins PHGDH was identified by iTRAQ proteomics technology. Experimental rat varicocele model was constructed according to our new clip technique, the mRNA and protein expression levels of PHGDH were examined with qRT-PCR and Western blotting. We constructed a sertoli cell of PHGDH down-regulation model, and then detected the glucose consumption, LDH activities and lactate production in the sertoli cells. Western blot was conducted to investigate the effects of PHGDH on the expression of phosphoserine phosphatase (PSPH) and Pyruvate kinase M2 (PKM2). Flow cytometry was used to detect the cell apoptosis and cell cycle in sertoli cells. Results: The results showed that testicular protein PHGDH was down-regulated in patients with varicocele and in experimental rat varicocele model. Down-regulation of PHGDH in sertoli cells significantly decreased the glucose consumption, LDH activities and lactate production in the sertoli cells, indicating that the low expression of PHGDH ultimately led to a decrease in lactate production by affecting the glycolysis. The Western blot results showed that the down-regulation of PHGDH significantly reduced the expression of pathway protein PSPH and PKM2, leading to the reduction of lactate production. Moreover, PHGDH knockdown can promote apoptosis and inhibit cell cycle to affect cell growth. Conclusions: Overall, we conformed that varicocele lead to the decreasing of testis lactate production. Downregulation of PHGDH in sertoli cells may mediate the process of abnormal glucose metabolism. Our study provide new insight into the mechanisms underlying metabolism-associated male infertility and suggests a novel therapeutic target for male infertility.

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With a new clip technique surgically inducing varicocele in Sprague-Dawley rats

Cited by 6 publications

References 20 publications

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Thymoquinone improves testicular damage and sperm quality in experimentally varicocele‐induced adolescent rats

Down regulating PHGDH affects the lactate production of sertoli cells in varicocele

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