Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Abstract

Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

Exosome Therapy on Cisplatin-Induced Testicular Tissue Toxicity

“…Exo therapy at a single dose signi cantly improved in tissue repair via regulating Cis-induced autophagy. Previous studies have showed that exos protected against testicular ischemia/reperfusion injury in rats through antioxidant, anti-in ammatory, and antiapoptotic mechanisms (43,44). It has also been reported that antitumoural drugs trigger the autophagy process.…”